联合作者(
Jianjun Sun University of Texas at El Paso El Paso
2 protocols

Salvador Vazquez-Reyes University of Texas at El Paso El Paso
1 protocol

Javier Aguilera
  • Research associate, University of Texas at El Paso El Paso
  • 已发表 protocol 2

实验室信息

Sun Lab

已发表 protocol 2篇
A Fluorescence Dequenching-based Liposome Leakage Assay to Measure Membrane Permeabilization by Pore-forming Proteins
一种以测定成孔蛋白膜通透性的基于荧光淬灭的脂质体渗漏测定方法

作者:Javier Aguilera, Salvador Vazquez-Reyes and Jianjun Sun日期:05/20/2021,浏览量:2667,Q&A: 0

Pore-forming toxins (PFTs) have been discovered in a wide range of organisms. Their functions are essential to the survival or virulence of many species. PFTs often interact with lipid membranes. Large unilamellar vesicles (LUV), also known as

...
Measuring Cytosolic Translocation of Mycobacterium marinum in RAW264.7 Macrophages with a CCF4-AM FRET Assay
用CCF4-AM FRET法检测RAW264.7巨噬细胞中海洋分枝杆菌的细胞质移位

作者:Javier Aguilera and Jianjun Sun日期:04/20/2021,浏览量:1341,Q&A: 0
The CCF4-AM Förster resonance energy transfer (FRET) assay is a sensitive approach to measure bacterial cytosolic translocation in live cells. The FRET pair hydroxycoumarin (donor) and fluorescein (acceptor) are linked by a CCF4-AM β-lactam ring, ...