联合作者(
Tatsuo Fukagawa
2 protocols

Kohei Nishimura
  • Research scientist, Nagoya Nagoya
  • 已发表 protocol 1
已发表 protocol 1篇
A Simple Method to Generate Super-sensitive AID (ssAID)-based Conditional Knockouts using CRISPR-based Gene Knockout in Various Vertebrate Cell Lines
小胶质细胞的分离及蛋白表达的流式细胞仪分析:避免小胶质细胞背景荧光的陷阱

作者:Kohei Nishimura and Tatsuo Fukagawa日期:07/20/2021,浏览量:722,Q&A: 0

Inducing loss of function of a target protein using methods such as gene knockout is a powerful and useful strategy for analyzing protein function in cells. In recent years, the CRISPR/Cas-9-based gene knockout technology has been widely used across

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