联合作者(
Salvador Vazquez-Reyes University of Texas at El Paso El Paso
1 protocol

Javier Aguilera University of Texas at El Paso El Paso
2 protocols

Jianjun Sun
  • Faculty, University of Texas at El Paso El Paso
研究方向
  • microbial pathogenesis, host-pathogen interaction, Mycobacterium tuberculosis, virulence factors, protein-membrane interaction
  • 已发表 protocol 2

教育背景

PhD, Medical College of Wisconsin, 2004

实验室信息

Jianjun Sun's Lab
https://www.utep.edu/science/sun/

发表论文

已发表 protocol 2篇
A Fluorescence Dequenching-based Liposome Leakage Assay to Measure Membrane Permeabilization by Pore-forming Proteins
一种以测定成孔蛋白膜通透性的基于荧光淬灭的脂质体渗漏测定方法

作者:Javier Aguilera, Salvador Vazquez-Reyes and Jianjun Sun日期:05/20/2021,浏览量:2652,Q&A: 0

Pore-forming toxins (PFTs) have been discovered in a wide range of organisms. Their functions are essential to the survival or virulence of many species. PFTs often interact with lipid membranes. Large unilamellar vesicles (LUV), also known as

...
Measuring Cytosolic Translocation of Mycobacterium marinum in RAW264.7 Macrophages with a CCF4-AM FRET Assay
用CCF4-AM FRET法检测RAW264.7巨噬细胞中海洋分枝杆菌的细胞质移位

作者:Javier Aguilera and Jianjun Sun日期:04/20/2021,浏览量:1325,Q&A: 0
The CCF4-AM Förster resonance energy transfer (FRET) assay is a sensitive approach to measure bacterial cytosolic translocation in live cells. The FRET pair hydroxycoumarin (donor) and fluorescein (acceptor) are linked by a CCF4-AM β-lactam ring, ...