联合作者(
Jerry W. Shay Department of Cell Biology, University of Texas Southwestern Medical Center, USA
3 protocols
联合评审(
Alba Blesa Universidad Francisco de Vitoria
29 protocols

Alka Mehra Institute of Genomics and Integrative Biology
40 protocols

Amriti Rajender Lulla UT MD Anderson Cancer Center
6 protocols

Ana S. Almeida University College Cork
8 protocols

编委
ilgen Mender
  • Post-Doc, University of Texas Southwestern Medical Center
研究方向
  • 癌症生物学
  • Immuno-oncology
  • 已发表 protocol 3
已发表 protocol 3篇
Telomere Restriction Fragment (TRF) Analysis
端粒限制酶片段(TRF)分析

作者:Ilgen Mender and Jerry W. Shay日期:11/20/2015,浏览量:17438,Q&A: 0
While telomerase is expressed in ~90% of primary human tumors, most somatic tissue cells except transiently proliferating stem-like cells do not have detectable telomerase activity (Shay and Wright, 1996; Shay and Wright, 2001). Telomeres ...
Telomerase Repeated Amplification Protocol (TRAP)
端粒酶重复扩增实验(TRAP)

作者:Ilgen Mender and Jerry W. Shay日期:11/20/2015,浏览量:16757,Q&A: 1
Telomeres are found at the end of eukaryotic linear chromosomes, and proteins that bind to telomeres protect DNA from being recognized as double-strand breaks thus preventing end-to-end fusions (Griffith et al., 1999). However, due to the ...
参审 protocol 6篇
Single-molecule Fluorescence Technique to Monitor the Co-transcriptional Formation of G-quadruplex and R-loop Structures
单分子荧光技术监测G-四链体和R-环结构的共转录形成
作者:Gunhyoung Lim and Sungchul Hohng日期:07/05/2021,浏览量:1642,Q&A: 0

G-quadruplexes (GQ) and R-loops are non-canonical nucleic acid structures related to gene regulation and genome instability that can be formed during transcription; however, their formation mechanisms remain elusive. To address this question, we

...
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Isolation, Purification and Characterization of Exosomes from Fibroblast Cultures of Skeletal Muscle
骨骼肌成纤维细胞培养物中外泌体的分离、纯化、鉴定
作者:Diantha van de Vlekkert, Xiaohui Qiu, Ida Annunziata and Alessandra d'Azzo日期:04/05/2020,浏览量:2924,Q&A: 0
Exosomes are dynamic nanovesicles secreted by virtually all cells and are present in all biological fluids. Given their highly heterogeneous content exosomes have been implicated in many physiological and pathological processes that they exert by ...
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