联合作者(
Suchetana Mukhopadhyay Biology, Indiana University, USA, USA,
1 protocol

Joseph Che-Yen Wang Department of Biology, Indiana University, USA, USA,
1 protocol

Jolene Ramsey Department of Biology, Indiana University, USA, USA,
1 protocol

Brian Bothner Department of Chemistry and Biochemistry, Montana State University, USA
2 protocols
联合评审(
Balaji Olety Amaranath University of Massachusetts medical School
3 protocols

Balasubramanian Venkatakrishnan Syngene International Limited
3 protocols

Daniela Boehm Gladstone Institute of Virology and Immunology
3 protocols

David Paul MRC Laboratory of Molecular Biology
42 protocols

编委
Vamseedhar Rayaprolu
  • Research scientist, La Jolla Institute for Allergy and Immunology
研究方向
  • 生物物理学
  • Structural virology with a focus on viral assembly, functionalization and biology
    Membrane biology focused on stoichiometry and function
  • 已发表 protocol 2

教育背景

PhD in Biochemistry, Montana State University, 2013

实验室信息

https://www.lji.org/faculty-research/labs/saphire/#overview

发表论文

已发表 protocol 2篇
Alphavirus Purification Using Low-speed Spin Centrifugation
低速旋转离心纯化甲病毒

作者:Vamseedhar Rayaprolu, Jolene Ramsey, Joseph Che-Yen Wang and Suchetana Mukhopadhyay日期:03/20/2018,浏览量:8467,Q&A: 2
Chemical and sedimentation procedures are used to purify virus particles. While these approaches are successful for wild-type viruses, they are often not feasible for purifying mutant viruses with assembly defects. We combined two published methods ...
Fluorometric Estimation of Viral Thermal Stability
荧光法预测病毒热稳定性

作者:Vamseedhar Rayaprolu, Shannon Kruse, Ravi Kant, Navid Movahed, Dewey Brooke and Brian Bothner日期:08/05/2014,浏览量:10505,Q&A: 1
Differential Scanning Fluorimetry (DSF) is a rapid, economical, and a straightforward technique for estimating the thermal stability of proteins. The principle involves the binding of a fluorescent dye to thermally exposed hydrophobic pockets of a ...
参审 protocol 8篇
In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication
真核先导链/后随链DNA复制的体外分析
作者:Grant Schauer, Jeff Finkelstein and Mike O’Donnell日期:09/20/2017,浏览量:7209,Q&A: 0
The eukaryotic replisome is a multiprotein complex that duplicates DNA. The replisome is sculpted to couple continuous leading strand synthesis with discontinuous lagging strand synthesis, primarily carried out by DNA polymerases ε and δ, ...
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Modification of 3’ Terminal Ends of DNA and RNA Using DNA Polymerase θ Terminal Transferase Activity
利用DNA聚合酶θ末端转移酶活性修饰DNA和RNA的3'末端
作者:Trung M. Hoang, Tatiana Kent and Richard T. Pomerantz日期:06/20/2017,浏览量:7232,Q&A: 0
DNA polymerase θ (Polθ) is a promiscuous enzyme that is essential for the error-prone DNA double-strand break (DSB) repair pathway called alternative end-joining (alt-EJ). During this form of DSB repair, Polθ performs terminal transferase activity ...
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主审 protocol 16篇
Image-based Quantification of Direct Cell-to-cell Transmission of Bovine Viral Diarrhea Virus
基于成像量化分析牛病毒性腹泻病毒的细胞间传播
作者:Fernando Merwaiss and Diego E. Alvarez日期:08/05/2019,浏览量:2948,Q&A: 0
Different viruses rely on direct cell-to-cell transmission to propagate infection within the infected host. Measuring this mode of transmission in cultured cells is often complicated by the contribution of cell free viruses to spread, and the ...
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Analysis of Functional Virus-generated PAMP RNAs Using IFNα/β ELISA Assay
利用干扰素α/β酶联免疫吸附试验检测分析病毒产生的功能性PAMP RNAs
作者:Margit Mutso, Xiang Liu, Andres Merits and Suresh Mahalingam日期:06/20/2019,浏览量:2745,Q&A: 0
Virus-generated PAMP RNAs are key factors that activate host immune response. The PAMP RNAs are therefore usually closely related with viral disease pathogenesis. Quantitative real time PCR is a conventional method to assess RNA. However, it cannot ...
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