联合评审(
Alessandro Didonna University of California, San Francisco
51 protocols

Alka Mehra Institute of Genomics and Integrative Biology
40 protocols

Chhuttan L Meena National Chemical laboratory NCL Pune
2 protocols

David A. Cisneros Umeå University
33 protocols

评审
Ran Chen
  • CEO, Zhejiang JFK Biological Technology Co. Ltd.
研究方向
  • 分子生物学
  • 已发表 protocol 7
已发表 protocol 7篇
Isolation and Culture of Mouse Bone Marrow-derived Macrophages (BMM’phi’)
小鼠骨髓来源的巨噬细胞(BMM’phi’)的分离培养

作者:Ran Chen日期:02/05/2012,浏览量:63117,Q&A: 15
Bone marrow derived macrophages are a type of white blood cell that can be isolated from mammalian bone marrow. In this protocol, a method is described in which bone marrow cells are isolated from mouse leg bones (femur and tibia), and then ...
MTT Assay of Cell Numbers after Drug/Toxin Treatment
MTT法测定药物/毒物处理后的细胞数量

作者:Ran Chen日期:04/05/2011,浏览量:23446,Q&A: 1
MTT assay is a colorimetric method for measuring the activity of enzymes in living cells that reduce MTT to formazan dyes, giving a purple color. It is commonly used to determine cytotoxicity of potential medicinal agents and toxic materials, since ...
参审 protocol 6篇
Optimised Method for the Production and Titration of Lentiviral Vectors Pseudotyped with the SARS-CoV-2 Spike
SARS-CoV-2刺突慢病毒伪型载体制备和滴定的优化方法

The use of recombinant lentivirus pseudotyped with the coronavirus Spike protein of SARS-CoV-2 would circumvent the requirement of biosafety-level 3 (BSL-3) containment facilities for the handling of SARS-CoV-2 viruses. Herein, we describe a fast

...
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Optimised Method for the Production and Titration of Lentiviral Vectors Pseudotyped with the SARS-CoV-2 Spike
SARS-CoV-2假型慢病毒载体制备及滴定方法的优化

The use of recombinant lentivirus pseudotyped with the coronavirus Spike protein of SARS-CoV-2 would circumvent the requirement of biosafety-level 3 (BSL-3) containment facilities for the handling of SARS-CoV-2 viruses. Herein, we describe a fast

...
查看全文 >