联合作者(
Hajime Wada Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Japan
2 protocols

Shin-ya Miyagishima Department of Cell Genetics, National Institute of Genetics, Japan
2 protocols
联合评审(
Agnieszka Zienkiewicz Nicolaus Copernicus University
23 protocols

Ali Asghar Kermani University of Michigan, Ann Arbor
8 protocols

Arsalan Daudi University of California
157 protocols

Baohua Li University of California, Davis
4 protocols

评审
Kumiko Okazaki
  • Assistant professor, Hiroshima University
研究方向
  • 植物科学
  • 已发表 protocol 2

教育背景

Ph.D. (Science), The University of Tokyo

已发表 protocol 2篇
Preparation of Chloroplast Lipid Membrane and Lipid-Protein Interaction Assay
叶绿体膜脂的制备及脂质-蛋白质相互作用的分析

作者:Kumiko Okazaki, Shin-ya Miyagishima and Hajime Wada日期:02/05/2016,浏览量:7927,Q&A: 0
Lipid-Protein interaction assay is a method to search lipids, which are bound with proteins in vitro. Since membranes that are spotted with chloroplast lipids such as monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), ...
Measurement of PI4P Levels in Intact Chloroplasts Isolated from Arabidopsis thaliana
测量从拟南芥中分离的完整叶绿体中的PI4P含量

作者:Kumiko Okazaki, Shin-ya Miyagishima and Hajime Wada日期:02/05/2016,浏览量:7873,Q&A: 2
Phosphatidylinositol 4-phosphate (PI4P), a major species of phosphoinositides, modulates many fundamental cellular processes. We have recently revealed that PI4P plays an important role in chloroplast division as a negative regulator. Despite its ...
参审 protocol 5篇
A Sensitive Coupled Enzyme Assay for Measuring Kinase and ATPase Kinetics Using ADP-Specific Hexokinase
使用ADP特异性己糖激酶测量激酶和ATPase动力学的灵敏耦合酶活测定
作者:Ciaran R. McFarlane and James W. Murray日期:05/05/2020,浏览量:3363,Q&A: 0
Kinases and ATPases perform essential biological functions in metabolism and regulation. Activity of these enzymes is commonly measured by coupling ATP consumption to the synthesis of a detectable product. For most assay systems the ATP ...
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Fusarium graminearum Maize Stalk Infection Assay and Associated Microscopic Observation Protocol
玉米秸秆禾谷镰刀菌感染分析和显微镜观察
作者:Juan He, Tinglu Yuan and Wei-Hua Tang日期:12/05/2016,浏览量:9059,Q&A: 0
The ascomycete fungus Fusarium graminearum (previously also called Gibberella zeae) causes Gibberella stalk rot in maize (Zea mays) and results in lodging and serious yield reduction. To develop methods to assess ...
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