联合作者(
Samuel H. Wilson Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, USA
3 protocols

Julie K. Horton Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, USA
1 protocol
联合评审(
Alexandros Alexandratos National and Kapodistrian University of Athens
51 protocols

Chong He Buck Institute for Research on Aging
7 protocols

Gal Haimovich Molecular Genetics, Weizmann Institute of Science, Israel
101 protocols

Gregory C. Finnigan Kansas State University
2 protocols

评审
Melike Çağlayan
  • Assist. Prof., University of Florida
研究方向
  • 生物化学
  • 已发表 protocol 3

教育背景

Ph.D.

实验室信息

Caglayan Lab

发表论文

https://caglayan.biochem.med.ufl.edu
1) Caglayan M. and Wilson S.H. Pol μ dGTP mismatch insertion opposite T coupled with ligation reveals a promutagenic DNA intermediate during double strand break repair. Nature Communications (2018) 9: 4213.
2) Horton J.K., Stefanick D.F., Caglayan M., Zhao M.L., Gassman N.R., Wilson S.H. XRCC1 phosphorylation affects aprataxin recruitment and DNA deadenylation activity. DNA Repair (2018) – 64: 26 – 33.
3) Prasad R., Caglayan M., Da-Peng D., Nadalutti C.A., Gassman N.R., Zhao M., Stefanick D.F., Horton J.K., Krasich R., Longley M.J., Copeland W.C., Griffith J.D., Wilson S.H. DNA polymerase β: The missing link of the base excision repair machinery in mammalian mitochondria. DNA Repair (2017) 60: 77 – 88.
4) Caglayan M., Prasad R., Krasich R., Longley M.J., Kadoda K., Tsuda M., Sasanuma H., Takeda S., Tano K., Copeland W.C., Wilson S.H. Complementation of aprataxin deficiency by base excision repair enzymes in mitochondrial extracts. Nucleic Acids Research (2017) 17: 10079 – 10088.
5) Caglayan M., Wilson S.H. Role of DNA polymerase β oxidized nucleotide insertion in DNA ligation failure. Journal of Radiation Research - Review article (2017) 1093: 1 – 5.
6) Caglayan M., Horton J.K., Da-Peng D., Stefanick D.F., Wilson S.H. Oxidized nucleotide insertion by pol β confounds ligation during base excision repair. Nature Communications (2017) 8: 14045.
7) Sassa A., Caglayan M., Rodriguez Y., Beard W.A., Wilson S.H., Nohmi T., Honma M., Yasui M. Impact of ribonucleotide backbone on translesion synthesis and repair of 7,8-Dihydro-8-oxoguanine. Journal of Biological Chemistry (2016) 291: 24314 – 24323.
8) Caglayan M., Wilson S.H. Oxidant and environmental toxicant-induced effects compromise DNA ligation during base excision DNA repair. DNA Repair - Review article (2015) 35: 85 – 89.
9) Caglayan M., Horton J.K., Prasad R., Wilson S.H. Complementation of aprataxin deficiency by base excision repair enzymes. Nucleic Acids Research (2015) 43: 2271 – 2281.
10) Caglayan M., Batra V.K., Sassa A., Prasad R., Wilson S.H. Role of polymerase β in complementing aprataxin deficiency during abasic-site base excision repair. Nature Structural and Molecular Biology (2014) 21: 497 – 499.
11) Sassa A., Caglayan M., Dyrkheeva N.S., Beard W.A., Wilson S.H. Base excision repair of tandem modifications in a methylated CpG dinucleotide. Journal of Biological Chemistry (2014) 289: 13996 – 4008.
已发表 protocol 3篇
In vitro Assay to Measure DNA Polymerase β Nucleotide Insertion Coupled with the DNA Ligation Reaction during Base Excision Repair
体外实验检测在碱基去除修复中聚合酶β联合DNA连接反应介导的核苷酸插入

作者:Melike Çağlayan and Samuel H. Wilson日期:06/20/2017,浏览量:6100,Q&A: 0
We previously reported that oxidized nucleotide insertion by DNA polymerase β (pol β) can confound the DNA ligation step during base excision repair (BER) (Çağlayan et al., 2017). Here, we describe a method to investigate pol β nucleotide ...
Enzymatic Activity Assays for Base Excision Repair Enzymes in Cell Extracts from Vertebrate Cells
脊椎动物细胞提取物的碱基切除修复酶的酶活性检测

作者:Melike Çağlayan, Julie K. Horton and Samuel H. Wilson日期:06/05/2015,浏览量:7082,Q&A: 0
We previously reported enzymatic activity assays for the base excision repair (BER) enzymes DNA polymerase β (pol β), aprataxin (APTX), and flap endonuclease 1 (FEN1) in cell extracts from Saccharomyces cerevisiae (Çağlayan and Wilson, ...
参审 protocol 4篇
Purification and HPLC Analysis of Cell Wall Muropeptides from Caulobacter crescentus
新月形杆菌细胞壁膜多肽的纯化和HPLC分析
作者:Gabriele Stankeviciute and Eric A. Klein日期:11/05/2019,浏览量:2863,Q&A: 0
The peptidoglycan sacculus, or cell wall, is what defines bacterial cell shape. Cell wall composition can be best characterized at the molecular level by digesting the peptidoglycan murein polymer into its muropeptide subunits and quantifying the ...
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RNA Capping by Transcription Initiation with Non-canonical Initiating Nucleotides (NCINs): Determination of Relative Efficiencies of Transcription Initiation with NCINs and NTPs
利用非典型启动核苷酸(NCIN)进行转录起始的RNA加帽:利用NCIN和NTP测定转录起始的相对效率
作者:Jeremy G. Bird, Bryce E. Nickels and Richard H. Ebright日期:06/20/2017,浏览量:6929,Q&A: 0
It recently has been established that adenine-containing cofactors, including nicotinamide adenine dinucleotide (NAD+), reduced nicotinamide adenine dinucleotide (NADH), and 3’-desphospho-coenzyme A (dpCoA), can serve as ‘non-canonical ...
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