• 卷册 11, 2021
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生物化学

Quantitative Analysis of Cellular Diacylglycerol Content 细胞中甘油二酯含量的量化分析 作者:Fikadu G. Tafesse, Karin Strijbis and Hidde L. Ploegh日期:08/05/2014,浏览量:10419,Q&A: 0
Diacylglycerol (DAG) is a bioactive lipid with diverse biological roles. DAG transiently accumulates in a membrane upon receipt of an appropriate stimulus that activates phospholipase C to cleave phospholipids. The resulting hydrolysis product DAG binds to proteins such as protein kinase C to initiate a variety of downstream cellular processes. ...
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免疫学

Generation and Maturation of Human Monocyte-derived DCs 由人单核细胞诱导树突细胞的产生和成熟 作者:Michela Spadaro, Monica Montone and Federica Cavallo日期:08/05/2014,浏览量:21415,Q&A: 0
Dendritic cells (DC) are antigen-presenting cells, which play a critical role in the regulation of the adaptive immune response. They act as a bridge between the innate and the adaptive immune systems. An approach to study their function and potentiality is to generate DC-like cells by culturing CD14+ monocyte-enriched peripheral blood ...
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微生物学

Purification of Herpesvirus Virions and Capsids 疱疹病毒和病毒衣壳的纯化 作者:Xinghong Dai and Z. Hong Zhou日期:08/05/2014,浏览量:14773,Q&A: 2
This protocol was designed for large-scale purification of herpesvirus particles by cell culture. Virions and capsids are isolated from extracellular culture media and cell nuclei, respectively. Purity and concentration of the purified samples are usually sufficient for structural studies with cryo electron microscopy and cryo electron tomography. ...
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Infectious Virus Yield Assay for Hepatitis E Virus 戊型肝炎病毒的感染病毒产量分析 作者:Yannick Debing, Kai Dallmeier and Johan Neyts日期:08/05/2014,浏览量:8889,Q&A: 0
Hepatitis E virus (HEV) is one of the main causes of acute hepatitis worldwide. Infections are particularly severe in pregnant women and chronic hepatitis E is known to occur in immunocompromised patients. Current therapy (ribavirin or pegylated alpha interferon) has severe side effects and cannot be employed in all patients. In order to evaluate ...
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Luminescence-based Antiviral Assay for Hepatitis E Virus 针对戊型肝炎病毒的发光抗病毒物质试验 作者:Yannick Debing, Kai Dallmeier and Johan Neyts日期:08/05/2014,浏览量:8367,Q&A: 0
Hepatitis E virus (HEV) is one of the main causes of acute hepatitis worldwide. Infections are particularly severe in pregnant women and chronic hepatitis E is known to occur in immunocompromised patients. Current therapy (ribavirin or pegylated alpha interferon) has severe side effects and cannot be employed in all patients. In order to evaluate ...
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Quantification of Flavin Production by Bacteria 细菌黄素产量的定量测定 作者:Svetlana N. Yurgel, Joseph Lynch, Jennifer Rice, Neil Adhikari and Sanja Roje日期:08/05/2014,浏览量:8637,Q&A: 0
This protocol provides a simple and fast method of quantification for intracellular flavin content, and for flavin secretion by bacteria. Intracellular flavins are extracted from bacterial pellets, and secreted flavins are examined in the cell growth medium. Flavins are separated and measured using HPLC with fluorescence detection, and quantified ...
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Assay for GTP Cyclohydrolase II Activity in Bacterial Extracts 细菌提取物中GTP环化水解酶II活性分析 作者:Svetlana N. Yurgel, Na Sa, Jennifer Rice and Sanja Roje日期:08/05/2014,浏览量:6142,Q&A: 0
Riboflavin is the precursor of flavin nucleotides FMN and FAD, they play significant roles in all organisms. GTP is the initial precursor on riboflavin biosynthesis pathway and GTP cyclohydrolase II catalyzes the first step of this pathway. It converts GTP to 2,5-diamino-6-ribosylamino-4 (3H) -pyrimidinone 5'-phosphate. This protocol ...
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Fluorometric Estimation of Viral Thermal Stability 荧光法预测病毒热稳定性 作者:Vamseedhar Rayaprolu, Shannon Kruse, Ravi Kant, Navid Movahed, Dewey Brooke and Brian Bothner日期:08/05/2014,浏览量:10504,Q&A: 1
Differential Scanning Fluorimetry (DSF) is a rapid, economical, and a straightforward technique for estimating the thermal stability of proteins. The principle involves the binding of a fluorescent dye to thermally exposed hydrophobic pockets of a protein. The dyes used in this technique are highly fluorescent in a non-polar environment and are ...
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植物科学

Isolation of Tomato Fruit Chromoplasts and Determination of ATP Levels 番茄果实有色体的分离和ATP含量的测定 作者:Francesc Hernández-Gras, Rocco Petrizzo, Irini Pateraki, Marta Renato, Maxime Angaman, Joaquín Azcón-Bieto and Albert Boronat日期:08/05/2014,浏览量:8925,Q&A: 0
It has recently been reported that tomato fruit chromoplasts can synthesize ATP de novo using an ATP synthase complex harboring an atypical γ-subunit which is also present in a variety of plant species. However many aspects related with the biochemical processes underlying this process remain largely unknown. Here we describe detailed ...
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Quantifying Fruit Dehiscence Using the Random Impact Test (RIT) 采用随机碰撞试验(RIT)定量测定果实开裂 作者:Teresa Lenser and Günter Theißen日期:08/05/2014,浏览量:7428,Q&A: 0
Fruit dehiscence is an important evolutionary and agronomic trait. For quantifying and comparing the exact fruit dehiscence capability between individual plants, the random impact test has been described (Morgan et al., 1998; Bruce et al., 2002; Arnaud et al., 2010). Here, we describe the random impact test optimized to ...
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Floral Dip Transformation in Lepidium campestre 花器官浸蘸法转化绿独行菜 作者:Teresa Lenser and Günter Theißen日期:08/05/2014,浏览量:9305,Q&A: 0
Floral dip is a very common technique to stably transform Arabidopsis thaliana (Clough and Bent, 1998; Martinez-Trujillo et al., 2004; Zhang et al., 2006) and has also been adapted to some other plant species (Curtis and Nam, 2001; Tague, 2001; Bartholmes et al., 2008). Here, we describe this method optimized ...
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DNA Fragmentation Analysis DNA片段化分析 作者:Kei-ichiro Mishiba, Yukihiro Nagashima, Noriko Hayashi and Nozomu Koizumi日期:08/05/2014,浏览量:15447,Q&A: 0
DNA fragmentation with length corresponding to multiple integer of approximately 180 base pairs is a distinct feature of apoptosis in animals and programmed cell death in plants. This feature can simply be detected by DNA gel electrophoresis followed by ethidium bromide staining, although in some cases it is difficult to distinguish the DNA ...
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