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癌症生物学

Vaccine-induced Cytokine Production Detected by Luminex Multiplex Analysis Lunimex 多因子分析试剂盒检测疫苗诱导的细胞因子的生成 作者:Rachel Perret, Sophie R. Sierro, Natalia K. Botelho, Stephanie Corgnac, Alena Donda and Pedro Romero日期:08/20/2014,浏览量:8153,Q&A: 0
Different vaccine and adjuvant combinations are known to rapidly induce antigen presenting cell (APC) maturation and pro-inflammatory cytokine and production, which in turn play an important role in the priming of antigen-specific T cells. Measuring cytokine production systemically in the serum fails to detect localized responses in the lymph ...
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Analysis of Tumor-infiltrating Lymphocytes Following CD45 Enrichment CD45富集后的肿瘤浸润淋巴细胞的分析 作者:Rachel Perret, Sophie R. Sierro, Natalia K. Botelho, Stephanie Corgnac, Alena Donda and Pedro Romero日期:08/20/2014,浏览量:22702,Q&A: 0
Measuring antigen-specific T cell responses in the blood and lymphoid organs of vaccinated mice can give us a useful indication of the potency of a vaccine formulation. Unfortunately, systemic or even localized lymphoid T cell responses are not always predictive of the ability of a vaccine to induce tumor protection. Measuring the antigen-specific ...
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免疫学

Enzymatic Reactions and Detection of C3 Cleavage Fragments 通过C3被切割后产生的片段检测C3转化酶的活性 作者:Claudia Kemper and Martin Kolev日期:08/20/2014,浏览量:9604,Q&A: 0
The complement component C3 is the major effector molecule of the complement system. C3 circulates in the blood and interstitial fluids as pro-enzyme and is activated by enzymatic cleavage into a C3a portion, a classic anaphylatoxin that functions as chemoattractant and immune cell activator, and the C3b portion, the body’s most potent opsonin. C3 ...
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Signaling Assays for Detection of Human G-protein-coupled Receptors in Yeast 以酵母为模型对人G蛋白偶联受体进行信号传导分析 作者:Yasuyuki Nakamura, Jun Ishii and Akihiko Kondo日期:08/20/2014,浏览量:9219,Q&A: 0
G-protein-coupled receptors (GPCRs) are the largest group of cell-surface proteins and are major molecular targets for drug development. The protocol described herein is for the detection of human GPCR signaling in the yeast Saccharomyces cerevisiae. Using Zoanthus sp. green fluorescent protein (ZsGreen) as the reporter, ...
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微生物学

In vitro DNA Polymerization Activity Assay Using Cell-free Extracts 采用细胞抽提液进行体外DNA聚合活性测试 作者:Anurag K. Sinha and Malay K. Ray日期:08/20/2014,浏览量:7478,Q&A: 0
This protocol has been designed to measure the in-vitro DNA polymerization activity in crude cell extracts of the Antarctic bacterium Pseudomonas syrinagae Lz4W. This bacterium can grow at 4 °C with optimum growth rate at 22 °C. The slow growth rate of the bacterium observed at low temperature (4 °C) compared to higher ...
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Endolysin Expression, Purification and Activity Determination by Zymography 酶谱法进行细胞内溶素表达、纯化和活性测定 作者:Alexander B. Westbye, Paul C. Fogg and John Thomas Beatty日期:08/20/2014,浏览量:13118,Q&A: 0
Endolysins are peptidoglycan-degrading (muralytic) enzymes produced by many bacteriophages for cell lysis of the host bacterium. The enzymatic activity of muralytic enzymes can be assayed qualitatively using a zymogram containing incorporated peptidoglycan. This protocol describes the expression of a recombinant 6x His-tagged endolysin using an ...
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VZV Replication Assays 水痘带状疱疹复制分析 作者:Samantha J. Griffiths and Jürgen Haas日期:08/20/2014,浏览量:9635,Q&A: 0
Varicella zoster virus (VZV) is a human herpesvirus which causes Varicella (chickenpox) upon primary infection and Zoster (shingles) following reactivation from latency (von Bokay, 1909). Whilst VZV is extensively studied, inherent features of VZV replication, such as cell-association of virus particles during in vitro culture and a ...
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HIV-1 Fusion Assay HIV-1 细胞融合分析 作者:Marielle Cavrois, Jason Neidleman and Warner C. Greene日期:08/20/2014,浏览量:13575,Q&A: 0
The HIV-1 fusion assay measures all steps in the HIV-1 life cycle up to and including viral fusion. It relies on the incorporation of a β-lactamase–Vpr (BlaM-Vpr) protein chimera into the virion and the subsequent transfer of this chimera into the target cell by fusion (Figure 1). The transfer is monitored by the enzymatic cleavage of CCF2, a ...
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神经科学

Elevated Plus Maze Test to Assess Anxiety-like Behavior in the Mouse 高架十字迷宫试验评估小鼠的焦虑样行为 作者:Luciana M. Leo and Fabricio A. Pamplona日期:08/20/2014,浏览量:25830,Q&A: 0
The elevated plus maze task is a simple method to assess anxiety-like behaviors in rodents. This version describes the procedure used in mice. However, the protocol may also be applied to rats, considering a proportionally larger apparatus (arms: 10 x 50 cm; height: 55 cm). Briefly, the test is performed on a plus-shaped apparatus with two open ...
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植物科学

Assays of Polyphenol Oxidase Activity in Walnut Leaf Tissue 核桃叶组织中的多酚氧化酶活性分析 作者:Ross Gertzen and Matthew A. Escobar日期:08/20/2014,浏览量:18967,Q&A: 0
Polyphenol oxidase (PPO) is an enzyme that catalyzes the hydroxylation of monophenols into ortho-diphenols (cresolase activity) and the oxidation of o-diphenols into quinones (catecholase activity) (Figure 1). These quinones spontaneously polymerize to form dark-colored phytomelanins, most often seen in the browning of damaged plant ...
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SGR-based Reporter to Assay Plant Transcription Factor-promoter Interactions 基于拟南芥滞绿报告基因(SGR)分析植物转录因子与启动子之间的相互作用 作者:Shisong Ma and Savithramma Dinesh-Kumar日期:08/20/2014,浏览量:9058,Q&A: 0
We developed an in vivo method to assay plant transcription factor (TF)–promoter interactions using the transient expression system in Nicotiana benthamiana (N. benthamiana) plants. The system uses the Arabidopsis stay green (SGR) gene as a reporter. Induction of SGR expression in N. ...
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Salinity Assay in Tomato 番茄耐盐实验 作者:Begoña Renau-Morata, Manuel Sánchez-Perales, Joaquín Medina, Rosa Victoria Molina, Rocío Corrales, Laura Carrillo, Pedro Fernández-Nohales, Jorge Marqués, Stephan Pollmann, Jesús Vicente-Carbajosa, Antonio Granell and Sergio G. Nebauer日期:08/20/2014,浏览量:13858,Q&A: 2
Tomato is one of the most important horticultural crops worldwide, and is cultivated in semi-arid regions in which soil and groundwater salinity is an increasing limitation to yield. The assessment of the responses of new cultivars to salt and the comparisons among cultivars and wild species are of great interest in tomato breeding. This assay ...
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Salinity Assay in Arabidopsis 拟南芥耐盐实验 作者:Alba Rocío Corrales, Laura Carrillo, Sergio G. Nebauer, Begoña Renau-Morata, Manuel Sánchez-Perales, Pedro Fernández-Nohales, Jorge Marqués, Antonio Granell, Stephan Pollmann, Jesús Vicente-Carbajosa, Rosa Victoria Molina and Joaquín Medina日期:08/20/2014,浏览量:16703,Q&A: 0
Salinity is an important environmental constraint to crop productivity in arid and semi-arid regions of the world. The evaluation of the responses to salinity of different Arabidopsis ecotypes or transgenic lines is important to identify and investigate the role of different key genes. These new characterized genes involved in the ...
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系统生物学

siRNA Screening for Genes Involved in HSV-1 Replication 对参与HSV-1病毒复制的基因进行siRNA筛选 作者:Samantha J. Griffiths and Jürgen Haas日期:08/20/2014,浏览量:9244,Q&A: 0
Small interfering RNAs (siRNAs) are small (typically 18-24 nucleotides) RNA molecules capable of silencing gene expression post-transcriptionally and as such, they provide a simple method by which the role of individual genes in complex cellular systems can be easily assessed. As siRNAs are easy to use experimentally, and can be designed to target ...
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