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癌症生物学

Hypoxia Studies with Pimonidazole in vivo 体内哌莫硝唑缺氧研究 作者:Kristina Y. Aguilera and Rolf A. Brekken日期:10/05/2014,浏览量:22944,Q&A: 0
Therapy-induced hypoxia drives changes in the tumor microenvironment that contribute to the poor response to therapy. Hypoxia is capable of driving the expression and/or activation of specific signaling cascades (e.g., c-Met, Axl, CTGF), the recruitment of tumor promoting immune cells, and the induction of cell survival pathways including ...
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免疫学

Coagulation Assay 血细胞凝集试验 作者:Praveen Papareddy, Martina Kalle and Artur Schmidtchen日期:10/05/2014,浏览量:12394,Q&A: 0
Clotting times can be measured by using citrate plasma. The intrinsic pathway of coagulation is measured by the activated partial thromboplastin time (aPTT), the extrinsic pathway of coagulation, monitored by measuring the prothrombin time (PT), and thrombin-induced fibrin-network formation (thrombin clotting time; TCT).
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Isolation and in vivo Transfer of Antigen Presenting Cells 抗原递呈细胞的分离和体内转移 作者:Pooja Arora, Shalu Sharma Kharkwal and Steven A. Porcelli日期:10/05/2014,浏览量:10827,Q&A: 0
Transfer of antigen presenting cells in vivo is a method used by immunologists to examine the potency of antigen presentation by a selected population of cells. This method is most commonly used to analyze presentation of protein antigens to MHC class I or II restricted T cells, but it can also be used for studies of nonconventional ...
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微生物学

Chromogenic Substrate Assay for Determining the Activity of Plasma Kallikrein 显色底物分析测定血浆激肽释放酶活性 作者:Praveen Papareddy, Martina Kalle and Artur Schmidtchen日期:10/05/2014,浏览量:8355,Q&A: 0
The activation of the intrinsic pathway takes place at negatively charged surfaces, such as bacteria, and involves activation of cogulation Factor XII, which then leads to the activation of plasma kallikrein (PK) and coagulation Factor XI. To determine the PK activity on bacterial surfaces, bacteria were pre-incubated with peptides, followed by ...
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Construction of Glycine Oxidase Mutant Libraries by Random Mutagenesis, Site Directed Mutagenesis and DNA Shuffling 通过随机突变、点位定向诱变和DNA改组构建甘氨酸氧化酶突变库 作者:Tao Zhan日期:10/05/2014,浏览量:8791,Q&A: 0
Glyphosate, a broad spectrum herbicide widely used in agriculture all over the world, inhibits 5-enolpyruvylshikimate-3-phosphate synthase in the shikimate pathway, and glycine oxidase (GO) has been reported to be able to catalyze the oxidative deamination of various amines and cleave the C-N bond in glyphosate (Pedotti et al., 2009). ...
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Expression and Purification of the Thermus thermophilus Argonaute Protein 嗜热细菌Argonaute蛋白的表达和纯化 作者:Daan C. Swarts, Matthijs M. Jore and John van der Oost日期:10/05/2014,浏览量:8974,Q&A: 0
The Argonaute protein of Thermus thermophilus (TtAgo) has recently been studied in detail. For its in vitro characterization, TtAgo was purified after heterologous expression in Escherichia coli (E. coli). As TtAgo expression is toxic, a tightly controlled system was used for protein ...
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神经科学

Novel Object Recognition for Studying Memory in Mice 采用新物体识别研究小鼠的记忆 作者:Tzyy-Nan Huang and Yi-Ping Hsueh日期:10/05/2014,浏览量:28867,Q&A: 0
Memory tests are important indexes of the brain functions for rodents behavior assay. Many memory tasks require external forces (e.g. electric shocks) or intrinsic forces (e.g. hunger and thirsty) to trigger the responses. Under those conditions, rodents are under stresses, such as pain, tired, malnutrition or dehydration, which ...
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Two-choice Digging Task in Mouse for Studying the Cognitive Flexibility 采用小鼠双选择挖掘任务研究认知灵活性 作者:Hsiu-Chun Chuang, Tzyy-Nan Huang and Yi-Ping Hsueh日期:10/05/2014,浏览量:11067,Q&A: 1
Cognitive flexibility, the higher-order cognition involving reversal learning, has been defined as having the ability to shift one’s previous thoughts or actions to new situations depending on situational demands. Studies of neuropsychiatric disorders such as autism spectrum disorder (ASD) showed that restricted and repetitive patterns of ...
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In utero Electroporation of the Embryonic Mouse Retina 小鼠子宫内胚胎视网膜DNA电转移 作者:Francisco Nieto-Lopez and Luisa Sanchez-Arrones日期:10/05/2014,浏览量:13060,Q&A: 0
This protocol is useful to manipulate gene expression in the embryonic retina and compare the result with the contralateral non electroporated retina. In addition, the electroporation of a membrane or cytoplasmic tagged GFP allows to determine the effects of gene manipulation on the outgrowth of retinal ganglion cell axons (Garcia-Frigola et al. ...
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植物科学

Analysis of Total Se Content in Rice 水稻中总硒含量的分析 作者:Lianhe Zhang, Feiyan Yu, Kun Deng, Bin Hu and Chengcai Chu日期:10/05/2014,浏览量:7005,Q&A: 0
Total Se content in rice is normally low and it is difficult to determine it exactly because of Se volatilization and pollution during the digestion process. In this method, rice sample is digested thoroughly and Se volatilization is reduced greatly by designing a specific digestion tube, increasing digestion temperature by three steps, ...
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Pea Aphid Survival Assays on Arabidopsis thaliana 拟南芥的豌豆蚜存活实验 作者:David C. Prince, Sam T. Mugford, Thomas R. Vincent and Saskia A. Hogenhout日期:10/05/2014,浏览量:8344,Q&A: 0
Aphids are phloem-feeding insects that successfully colonize specific host plant species. Aphid performance on a given plant is commonly measured by assessing fecundity of an aphid species that is adapted to the host. However, this approach may not reveal roles for plant genes in defense pathways that adapted aphids suppress. The host range of the ...
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Carotenoid Extraction and Quantification from Capsicum annuum 甜椒中的类胡萝卜素提取和定量测定 作者:Richard D. Richins, James Kilcrease, Laura Rodgriguez-Uribe and Mary A. O’Connell日期:10/05/2014,浏览量:11871,Q&A: 0
Carotenoids are ubiquitous pigments that play key roles in photosynthesis and also accumulate to high levels in fruit and flowers. Specific carotenoids play essential roles in human health as these compounds are precursors for Vitamin A; other specific carotenoids are important sources of macular pigments and all carotenoids are important ...
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Endogenous ABA Extraction and Measurement from Arabidopsis Leaves 拟南芥叶片中内源ABA的提取和测定 作者:Ning Liu, Yong Ding, Michael Fromm and Zoya Avramova日期:10/05/2014,浏览量:10426,Q&A: 0
The endogenous messenger, the phytohormone abscisic acid (ABA) plays a major role in plant’s adaption to drought, salinity, cold and other abiotic stresses. In addition to abiotic stress signaling, ABA is involved also in developmental regulation and in responses to diverse biotic stresses. Dehydration stress results in a strong increase in ...
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Agrobacterium tumefaciens-mediated Transformation of Walnut (Juglans regia) 农杆菌介导的核桃转化 作者:Sriema L. Walawage, Charles A. Leslie, Matthew A. Escobar and Abhaya M. Dandekar日期:10/05/2014,浏览量:11657,Q&A: 0
Like many woody plant species, walnut (Juglans regia) can be difficult to genetically transform and regenerate. However, somatic embryos have been used successfully for over two decades as a target tissue for transformation and regeneration of transgenic walnut plants. Walnut somatic embryos, initiated originally from developing zygotic ...
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Localisation and Quantification of Reactive Oxygen Species and Nitric Oxide in Arabidopsis Roots in Response to Fungal Infection 拟南芥根部响应真菌感染的活性氧和一氧化氮的定位和定量测定 作者:Kapuganti J. Gupta, Yariv Brotman and Luis A. J. Mur日期:10/05/2014,浏览量:10385,Q&A: 0
Nitric oxide and reactive oxygen species have emerged as important signalling molecules in plants. The half-lives of NO and ROS are very short therefore rapid and precise measurements are required for the understanding biological roles of these redox active species. Various organelles and compartments generate NO and ROS thus it is important to ...
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