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癌症生物学

Detection of ALT Associated Promyelocytic Leukemia Nuclear Bodies (APBs) by Immunofluorescence-FISH (IF-FISH) 免疫荧光原位杂交法(IF-FISH)检测ALT相关的前髓细胞性白血病核体(APB) 作者:Siamak A. Kamranvar and Maria G. Masucci日期:12/05/2014,浏览量:11204,Q&A: 0
The activation of functions that counteract the physiological shortening of telomeres in rapidly proliferating cell is prerequisite for the progression of cancer cells to full malignancy (Collado et al., 2007). In most human cancers, the length of telomere is maintained through up-regulation of telomerase whereas a telomerase-independent ...
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细胞生物学

TGFβ Stimulation Assay TGFβ刺激试验 作者:Emanuel Rognoni日期:12/05/2014,浏览量:13387,Q&A: 0
TGFβ is part of a growth factor superfamily which modulates cell growth, differentiation, adhesion, migration, ECM synthesis and apoptosis (Massague, 1998; Siegel and Massague, 2003). Free TGFβ binds to its high affinity TGFβ receptor, a receptor serine/threonine kinase, inducing phosphorylation of Smad2/3 which subsequently forms a complex with ...
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TGFβ Release Co-culture Assay TGFβ 释放共培养试验 作者:Emanuel Rognoni日期:12/05/2014,浏览量:9779,Q&A: 0
TGFβ is a potent cytokine modulating various processes including proliferation, differentiation, ECM synthesis and apoptosis (Siegel and Massague, 2003). Thus in many tissues availability of TGFβ is tightly regulated. TGFβ is secreted as an inactive complex where it is encapsulated by the latency associated protein (LAP), a ligand trap protein, ...
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微生物学

Visualization of Cell Complexity in the Filamentous Cyanobacterium Mastigocladus laminosus by Transmission Electron Microscopy (TEM) 透射电镜(TEM)法观察分析丝状蓝细菌层理鞭枝藻细胞复杂度 作者:Dennis J. Nürnberg, Giulia Mastroianni, Conrad W. Mullineaux and Graham D. McPhail日期:12/05/2014,浏览量:8555,Q&A: 0
The cyanobacterium Mastigocladus laminosus (M. laminosus) is one of the most morphologically complex prokaryotes. It forms long chains of cells that are connected via septal junction complexes; such complexes allow diffusion of metabolites and regulators between neighboring cells. Cellular division occurs in multiple planes, ...
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Induction of Tigecycline Resistance in Acinetobacter baumannii 鲍氏不动杆菌中替加环素抗性的诱导 作者:Ming-Feng Lin, Yun-You Lin and Chung-Yu Lan日期:12/05/2014,浏览量:7383,Q&A: 0
Multidrug resistance Acinetobacter baumannii (A. baumannii) (MDRAB) has emerged as a serious threat in hospitals in recent years. Currently, there are few antibiotics, including tigecycline, available to treat infections caused by MDRAB effectively. Both tigecycline-resistant and tigecycline-susceptible isogenic strains of MDRAB ...
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Minimal Inhibitory Concentration (MIC) Assay for Acinetobacter baumannii 鲍氏不动杆菌的最小抑菌浓度(MIC)测定 作者:Ming-Feng Lin, Yun-You Lin and Chung-Yu Lan日期:12/05/2014,浏览量:44011,Q&A: 3
Minimal inhibition concentration (MIC) is the lowest concentration of an antimicrobial agent that can inhibit the visible growth of a microorganism after overnight incubation. MIC determination is used as not only a diagnostic tool in treating bacterial infections for clinicians but also a research method in evaluating the efficacy of an ...
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Enzymatic Activity Assays in Yeast Cell Extracts 酵母细胞提取物的酶活性分析 作者:Melike Çağlayan and Samuel H. Wilson日期:12/05/2014,浏览量:9851,Q&A: 0
Saccharomyces cerevisiae (S. cerevisiae) (commonly known as baker’s yeast) is a model organism that has a similar upstream base excision repair (BER) pathway for the repair of methylated bases as that in mammalian cells, and it is very easy to maintain in the laboratory environment. Here, we described a method to prepare cell ...
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神经科学

Fluorescent Measurement of Synaptic Activity Using SynaptopHluorin in Isolated Hippocampal Neurons 采用SynaptopHluorin在分离海马神经元中进行突触活性荧光测量 作者:Hongmei Li, Han-A Park and Elizabeth A. Jonas日期:12/05/2014,浏览量:9704,Q&A: 0
This protocol comprises the entire process of fluorescent measurement of vesicle recycling using the probe SynaptopHluorin, a pH-dependent GFP variant whose fluorescence increases at the synapse upon vesicle release due to fluorescence quenching in acidic vesicles. This technique provides a genetic tool to monitor synaptic vesicle recycling in ...
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Creating a Rat Model of Chronic Variate Stress 大鼠慢性多种可变应激模型创建 作者:Rocío M. de Pablos, Manuel Sarmiento and Ana María Espinosa-Oliva日期:12/05/2014,浏览量:9981,Q&A: 0
Stress is a condition of human experience and an important factor in the onset of various diseases. There are numerous studies showing how stress can accelerate cell aging, immune senescence and some age-related diseases such as neurodegenerative disorders and osteoporosis. However, the effects of stress have different consequences depending on ...
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植物科学

In situ Digestion of Wheat Cell-wall Polysaccharides 小麦细胞壁多糖的原位消化 作者:Dušan Veličković and Hélène Rogniaux日期:12/05/2014,浏览量:9767,Q&A: 2
Cell walls of the wheat endosperm are mostly composed of arabinoxylans (AX) and mixed (1→3), (1→4)-β-glucans (BG) (Saulnier et al., 2012). Here, we present an optimized protocol to degrade enzymatically these cell-wall polysaccharides into oligosaccharides, directly from wheat grain cross sections. The main difficulty is to provide a ...
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Vanillin Assay of Arabidopsis Seeds for Proanthocyanidins 香草醛法检测拟南芥种子的原花青素 作者:Lijie Xuan, Zhong Wang and Lixi Jiang日期:12/05/2014,浏览量:9339,Q&A: 0
Proanthocyanidins (PAs) are colorless flavonoid polymers and deposit in Arabidopsis seed coat specifically. Oxidation of PAs gives rise to brown color of mature seeds. PA accumulation can be affected by a number of growth conditions, such as temperature and sun light. PAs, which are converted from anthocyanidins, can protect seeds from ...
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CytoTrap Two-Hybrid Screening Assay CytoTrap双杂交筛选系统 作者:Lei Zhang, Liqun Du and B. W. Poovaiah日期:12/05/2014,浏览量:10937,Q&A: 0
CytoTrap two-hybrid system provides an alternate strategy to detect protein-protein interactions in yeast. In this system, bait protein is fused with human son of sevenless (hSos) protein (Li et al., 1993), and a cDNA library or prey protein is expressed by fusion with myristoylation signal which anchors the prey fusion protein to yeast ...
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Infection Assays of Tomato and Apple Fruit by the Fungal Pathogen Botrytis cinerea 真菌病原体灰葡萄孢菌对番茄和苹果果实的感染分析 作者:Zhanquan Zhang, Guozheng Qin, Boqiang Li and Shiping Tian日期:12/05/2014,浏览量:11029,Q&A: 0
Botrytis cinerea (B. cinerea) is an aggressive fungal pathogen that infects more than 200 plant species. Furthermore, the pathogen can attack fruits of some plants, such as tomato and apple. B. cinerea has become one of the model systems in molecular phytopathology because of its economic importance and sophisticated ...
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Purification of Starch Granules from Arabidopsis Leaves and Determination of Granule-Bound Starch Synthase Activity 植物叶片淀粉粒的纯化和淀粉粒结合的淀粉合酶的活性分析 作者:Tomás Albi, M. Isabel Ortiz-Marchena, M. Teresa Ruiz, José M. Romero and Federico Valverde日期:12/05/2014,浏览量:7963,Q&A: 0
Starch constitutes the most important carbon reserve in plants and is composed of branched amylopectin and linear amylose. The latter is synthesized exclusively by the Granule-Bound Starch Synthase (GBSS, EC 2.4.1.21). Here we report a readily reproducible, specific and highly sensitive protocol, which includes the isolation of intact starch ...
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Determination of Soluble Sugars in Arabidopsis thaliana Leaves by Anion Exchange Chromatography 阴离子交换色谱法测定拟南芥中的可溶性糖 作者:M. Isabel Ortiz-Marchena, M. Teresa Ruiz, Federico Valverde and José M. Romero日期:12/05/2014,浏览量:12499,Q&A: 1
Determination of soluble sugars is basic for the study of carbon metabolism in plants. Soluble sugar quantitation can be achieved by enzymatic methods implying different coupled reactions. Here we describe a simple method that allows rapid determination of the most abundant soluble sugars (glucose, fructose and sucrose) in Arabidopsis ...
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