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生物化学

RNA Chromatin Immunoprecipitation (RNA-ChIP) in Caenorhabditis elegans 秀丽隐杆线虫的RNA染色质免疫共沉淀(RNA-ChIP) 作者:Germano Cecere and Alla Grishok日期:12/20/2014,浏览量:12631,Q&A: 0
The RNA chromatin immunoprecipitation assay (RNA-ChIP) allows detection and quantification of RNA–protein interactions using in vivo cross-linking with formaldehyde followed by immunoprecipitation of the RNA–protein complexes. Here we describe the RNA–ChIP protocol that we have adapted for Caenorhabditis elegans (C. elegans ...
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微生物学

Hepatitis C virus Cell-to-cell Spread Assay 丙型肝炎病毒在细胞之间的传播试验 作者:Naina Barretto and Susan L. Uprichard日期:12/20/2014,浏览量:9338,Q&A: 1
Hepatitis C virus (HCV) can infect naïve cells via entry of “cell-free” extracellular virus or direct “cell-to-cell” transmission. Here, we describe an assay for detecting HCV cell-to-cell transmission, using a non-growing cell culture system that avoids confounding effects of cell growth. The assay consists of infecting a small number of cells in ...
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Fluorescence-linked Antigen Quantification (FLAQ) Assay for Fast Quantification of HIV-1 p24Gag 荧光标记抗原法(FLAQ)对HIV-1 p24Gag快速定量 作者:Marianne Gesner, Mekhala Maiti, Robert Grant and Marielle Cavrois日期:12/20/2014,浏览量:9467,Q&A: 1
The fluorescence-linked antigen quantification (FLAQ) assay allows a fast quantification of HIV-1 p24Gag antigen. Viral supernatant are lysed and incubated with polystyrene microspheres coated with polyclonal antibodies against HIV-1 p24Gag and detector antibodies conjugated to fluorochromes (Figure 1). After washes, the ...
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植物科学

Cyclohexane Diamine Tetraacetic Acid (CDTA) Extraction of Plant Cell Wall Pectin 环己烷二胺四乙酸(CDTA)法提取植物细胞壁中的果胶 作者:Gerit Bethke and Jane Glazebrook日期:12/20/2014,浏览量:10158,Q&A: 0
The goal of this procedure is to extract pectin from plant cell walls. Pectins are galacturonic acid containing polymeric sugars that are important components of plant cell walls. Various procedures aimed at studying plant cell wall components require the extraction of pectin. Pectin is synthesized in the Golgi apparatus in a highly esterified ...
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Virus-induced Gene Silencing (VIGS) in Phalaenopsis Orchids 蝴蝶兰的病毒诱导基因沉默(VIGS) 作者:Ming-Hsien Hsieh, Zhao-Jun Pan, Hsin-Hung Yeh and Hong-Hwa Chen日期:12/20/2014,浏览量:10894,Q&A: 0
This is a protocol to produce stable silencing efficacy and efficiency for VIGS using CymMV as a silencing vector for floral functional genomics in Phalaenopsis orchids. This protocol is established based on a method created by Lu et al. (2007), and then modified by Hsieh et al. (2013a; 2013b), Lu et al. (2012) ...
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Imaging and Measurement of Nanomechanical Properties within Primary Xylem Cell Walls of Broadleaves 阔叶初生木质部细胞壁内的纳米力学性能的成像和测定 作者:Jaroslav Ďurkovič, Monika Kardošová and Rastislav Lagaňa日期:12/20/2014,浏览量:10837,Q&A: 0
A technique of atomic force microscopy (AFM) called PeakForce quantitative nanomechanical mapping (PeakForce QNM) is an efficient tool for the quantitative mechanobiological imaging of fibrillar aggregate, human epidermal cell and woody plant cell wall topography (Sweers et al., 2011; Heu et al., 2012; Ďurkovič et al., ...
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Trichome Isolation and Integrity Test from Brassica villosa and Other Species 芸薹属红丁香和其它物种的表皮毛分离和完整性检测 作者:Naghabushana Nayidu, Peta Bonham-Smith and Margaret Y. Gruber日期:12/20/2014,浏览量:8560,Q&A: 0
The outward growths from one or more epidermal cells are well known as trichomes (plant hair cells) (Levin, 1973; Mathur, 2006). Preparation of pure intact non-glandular trichomes from trichome-rich Brassica villosa depended on rendering the trichomes sufficiently stiff to dislodge them from the leaves in an undamaged state, which can be ...
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Agrobacterium-mediated Transformation of Mature Ginseng Embryos 农杆菌介导的成熟人参胚胎的遗传转化 作者:Ok Ran Lee, Jin Haeng Han and Yoon-Ri Kim日期:12/20/2014,浏览量:9982,Q&A: 0
Ginseng refers to species within the genus Panax and is a slow-growing perennial herb from the Araliaceae family. The most widely used Panax species is Panax ginseng Meyer (Korean ginseng). Panax japonicus (Japanese ginseng), Panax notoginseng (Chinese ginseng), and Panax quinquefolium (American ...
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Localization and Topology of Thylakoid Membrane Proteins in Land Plants 陆生植物类囊体膜蛋白的定位和拓扑结构 作者:Salar Torabi, Magdalena Plöchinger and Jörg Meurer日期:12/20/2014,浏览量:10696,Q&A: 0
Thylakoids are a formation of flattened membrane vesicles and protein complexes found in cyanobacteria, algae and plants. In the chloroplasts of land plants the thylakoid membrane systems form a network of densely packed stacks called grana lamellae, which are connected by unstacked stroma lamellae. Photosystem II is mainly localized in the ...
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Polysomal-mRNA Extraction from Arabidopsis by Sucrose-gradient Separation 采用甘蔗梯度分离法提取拟南芥多聚核糖体-mRNA 作者:Shih-Yun Lin and Guang-Yuh Jauh日期:12/20/2014,浏览量:10230,Q&A: 0
mRNAs surrounded by polysomes are ready for translation into proteins (Warner et al., 1963); these mRNAs are defined as polysomal-mRNAs (Mustroph et al., 2009). The process is affected by various growth conditions or surrounding situations. Microarray analysis is a powerful tool for detecting genome-wide gene expression. ...
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