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Image Credit: BioProtoc.2821

生物化学

Laminarin Quantification in Microalgae with Enzymes from Marine Microbes 海洋微生物酶法定量微藻中的昆布多糖 作者:Stefan Becker and Jan-Hendrik Hehemann日期:04/20/2018,浏览量:5543,Q&A: 0
The marine beta-glucan laminarin is an abundant storage polysaccharide in microalgae. High production rates and rapid digestion by heterotrophic bacteria turn laminarin into an ideal carbon and energy source, and it is therefore a key player in the marine carbon cycle. As a main storage glucan laminarin also plays a central role in the energy ...
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癌症生物学

FACS-based Glucose Uptake Assay of Mouse Embryonic Fibroblasts and Breast Cancer Cells Using 2-NBDG Probe 使用2-NBDG探针并基于FACS的小鼠胚胎成纤维细胞和乳腺癌细胞的葡萄糖摄取测定 作者:Shengli Dong and Suresh K Alahari日期:04/20/2018,浏览量:8266,Q&A: 0
This is a flow cytometry-based protocol to measure glucose uptake of mouse embryonic fibroblasts (MEFs) and breast cancer cells in vitro. The method is a slightly modified and updated version as previously described (Dong et al., 2017). Briefly, the target cells are incubated with the fluorescently tagged ...
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Generation of Luciferase-expressing Tumor Cell Lines 表达萤光素酶的肿瘤细胞系的生成 作者:Todd V. Brennan, Liwen Lin, Xiaopei Huang and Yiping Yang日期:04/20/2018,浏览量:14488,Q&A: 1
Murine tumor models have been critical to advances in our knowledge of tumor physiology and for the development of effective tumor therapies. Essential to these studies is the ability to both track tumor development and quantify tumor burden in vivo. For this purpose, the introduction of genes that confer tumors with bioluminescent ...
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细胞生物学

A Method for Extracting the Nuclear Scaffold from the Chromatin Network 一种从染色质网络中提取核骨架的方法 作者:Junjie Chen, Boon Heng Dennis Teo and Jinhua Lu日期:04/20/2018,浏览量:5665,Q&A: 0
Each cell contains many large DNA polymers packed in a nucleus of approx. 10 μm in diameter. With histones, these DNA polymers are known to form chromatins. How chromatins further compact in the nucleus is unclear but it inevitably depends on an extensive non-chromatin nuclear scaffold. Imaging of endogenous chromatin network and the complementary ...
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免疫学

3D Co-culture System of Tumor-associated Macrophages and Ovarian Cancer Cells 肿瘤相关巨噬细胞和卵巢癌细胞的3D共培养系统 作者:Lingli Long, Mingzhu Yin and Wang Min日期:04/20/2018,浏览量:7347,Q&A: 0
Ovarian cancer is fairly unique in that ovarian carcinoma cells can detach and spread directly through peritoneal cavity. It has been unclear, however, how detached cancer cells survive in the peritoneum and form spheroid structure. We have recently reported that there is a strong correlation between Tumor-associated macrophages (TAMs)-associated ...
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微生物学

Adhesion of Enteroaggregative E. coli Strains to HEK293 Cells 肠凝聚型大肠埃希杆菌对HEK293细胞的粘附测定 作者:Jorge Luis Ayala-Lujan and Fernando Ruiz-Perez日期:04/20/2018,浏览量:4950,Q&A: 0
Enteroaggregative Escherichia coli (EAEC) is a recognized cause of acute diarrhea among both children and adults worldwide. EAEC strains are characterized by the presence of aggregative adherence fimbriae (AAF), which play a key role in pathogenesis by mediating attachment to the intestinal mucosa and by triggering host inflammatory ...
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Quantification of Bacterial Twitching Motility in Dense Colonies Using Transmitted Light Microscopy and Computational Image Analysis 利用透射光学显微镜技术和计算机图像定量分析密集菌落中的细菌蹭行运动 作者:Benjamin Smith, Jianfang Li, Matteo Metruccio, Stephanie Wan, David Evans and Suzanne Fleiszig日期:04/20/2018,浏览量:5491,Q&A: 0
A method was developed to allow the quantification and mapping of relative bacterial twitching motility in dense samples, where tracking of individual bacteria was not feasible. In this approach, movies of bacterial films were acquired using differential interference contrast microscopy (DIC), and bacterial motility was then indirectly quantified ...
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Extraction of Small Molecules from Fecal Samples and Testing of Their Activity on Microbial Physiology 从粪便样本中提取小分子并检测其对微生物的生理学活性 作者:Eduardo S. Alves, Rosana B. R. Ferreira and L. Caetano M. Antunes日期:04/20/2018,浏览量:5621,Q&A: 0
The human body is colonized by vast communities of microbes, collectively known as microbiota, or microbiome. Although microbes colonize every surface of our bodies that is exposed to the external environment, the biggest collection of microbes colonizing humans and other mammals can be found in the gastrointestinal tract. Given the fact that the ...
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Determination of Intracellular Osmolytes in Cyanobacterial Cells 蓝藻细胞内渗透调节物质的测定 作者:Xiaoming Tan, Kuo Song, Cuncun Qiao and Xuefeng Lu日期:04/20/2018,浏览量:4374,Q&A: 0
Most of the cyanobacteria accumulate osmolytes including sucrose, glucosylglycerol, in their cells in response to salt stress. Here we describe a protocol of our laboratory for extraction and quantification of cyanobacterial intracellular sucrose and glucosylglycerol. We have confirmed this protocol was applicable to at least four kinds of ...
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Host-regulated Hepatitis B Virus Capsid Assembly in a Mammalian Cell-free System 宿主调控的乙型肝炎病毒衣壳在无哺乳动物细胞系统中的组装 作者:Kuancheng Liu and Jianming Hu日期:04/20/2018,浏览量:4887,Q&A: 0
The hepatitis B virus (HBV) is an important global human pathogen and represents a major cause of hepatitis, liver cirrhosis and liver cancer. The HBV capsid is composed of multiple copies of a single viral protein, the capsid or core protein (HBc), plays multiple roles in the viral life cycle, and has emerged recently as a major target for ...
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Method for CRISPR/Cas9 Mutagenesis in Candida albicans 白色念珠菌的CRISPR/Cas9诱变方法 作者:Neta Dean and Henry Ng日期:04/20/2018,浏览量:9225,Q&A: 0
Candida albicans is the most prevalent and important human fungal pathogen. The advent of CRISPR as a means of gene editing has greatly facilitated genetic analysis in C. albicans. Here, we describe a detailed step-by-step procedure to construct and analyze C. albicans deletion mutants. This protocol uses plasmids that ...
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Metal-tagging Transmission Electron Microscopy for Localisation of Tombusvirus Replication Compartments in Yeast 使用金属标签透射电子显微技术定位酵母菌中番茄丛矮病毒复制区 作者:Isabel Fernández de Castro and Cristina Risco日期:04/20/2018,浏览量:5114,Q&A: 0
Positive-stranded (+) RNA viruses are intracellular pathogens in humans, animals and plants. To build viral replicase complexes (VRCs) viruses manipulate lipid flows and reorganize subcellular membranes. Redesigned membranes concentrate viral and host factors and create an environment that facilitates the formation of VRCs within replication ...
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分子生物学

Generation of microRNA Sponge Library microRNA海绵文库的生成 作者:Sebastian Herzog日期:04/20/2018,浏览量:5287,Q&A: 0
This protocol describes the generation and functional validation of microRNA (miRNA) sponge or decoy constructs. When expressed from a strong promoter, these transcripts can sequester specific miRNA:RISC complexes, thereby resulting in a derepression of endogenous target mRNA. Hence, cells expressing such sponges display a partial or full miRNA ...
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神经科学

Electrophysiological Recordings of Evoked End-Plate Potential on Murine Neuro-muscular Synapse Preparations 电生理记录小鼠神经肌肉突触制备物中诱发的终板电位 作者:Giulia Zanetti, Samuele Negro, Aram Megighian and Marco Pirazzini日期:04/20/2018,浏览量:8911,Q&A: 0
Neuromuscular junction (NMJ) is the specialized chemical synapse that mediates the transmission of the electrical impulse running along motor neuron axons to skeletal muscle fibers. NMJ is the best characterized chemical synapse and its study along many years of research has provided most of the general knowledge of synapse development, structure ...
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Testing Effects of Chronic Chemogenetic Neuronal Stimulation on Energy Balance by Indirect Calorimetry 间接量热法测定慢性化学遗传神经元刺激对能量平衡的影响 作者:Sangho Yu and Heike Münzberg日期:04/20/2018,浏览量:4366,Q&A: 0
The fundamental of neuroscience is to connect the firing of neurons to physiological and behavioral outcomes. Chemogenetics enables researchers to control the activity of a genetically defined population of neurons in vivo through the expression of designer receptor exclusively activated by designer drug (DREADD) in specific neurons and ...
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Magnetic Resonance Imaging and Histopathological Visualization of Human Dural Lymphatic Vessels 人类硬脑膜淋巴管的磁共振成像及组织病理学观察 作者:Seung-Kwon Ha, Govind Nair, Martina Absinta, Nicholas J. Luciano and Daniel S. Reich日期:04/20/2018,浏览量:8444,Q&A: 2
In this protocol, we describe a method to visualize and map dural lymphatic vessels in-vivo using magnetic resonance imaging (MRI) and ex-vivo using histopathological techniques. While MRI protocols for routine imaging of meningeal lymphatics include contrast-enhanced T2-FLAIR and T1-weighted black-blood imaging, a more specific ...
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Isolation and Maintenance of Striatal Neurons 小鼠胚胎纹状体神经元的分离与维持 作者:Luana  Naia and A. Cristina Rego日期:04/20/2018,浏览量:7688,Q&A: 0
Primary cultures of murine striatal neurons are widely used to explore cellular mechanisms in neurobiology, including brain diseases. Here we describe a detailed and standardized protocol to dissect and culture embryonic murine striatal neurons GABA-positive/DARPP-32-positive for 12 days in vitro, when they show good neuronal cell ...
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植物科学

Quantification of Thrips Damage Using Ilastik and ImageJ Fiji 用Ilastik及ImageJ Fiji定量蓟马危害 作者:Isabella G. S. Visschers, Nicole M. van Dam and Janny L. Peters日期:04/20/2018,浏览量:7942,Q&A: 0
Quantification of insect damage is an essential measurement for identifying resistance in plants. In screening for host plant resistance against thrips, the total damaged leaf area is used as a criterion to determine resistance levels. Here we present an objective novel method for analyzing thrips damage on leaf disc using the freely available ...
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Qualitative Analysis of Lipid Peroxidation in Plants under Multiple Stress Through Schiff’s Reagent: A Histochemical Approach 植物多重应激下脂质过氧化作用的希夫试剂定性分析:组织化学方法 作者:Jay Prakash Awasthi, Bedabrata Saha, Bhaben Chowardhara, Sanjenbam Sanjibia Devi, Pankaj Borgohain and Sanjib Kumar Panda日期:04/20/2018,浏览量:10984,Q&A: 3
Lipid peroxidation is a physiological indicator of both biotic and abiotic stress responses, hence is often used as a biomarker to assess stress-induced cell damage or death. Here we demonstrate an easy, quick and cheap staining method to assess lipid peroxidation in plant tissues. In this methodology, Schiff’s reagent, is used to assay for ...
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In-vitro and in-planta Botrytis cinerea Inoculation Assays for Tomato 番茄离体及活体灰葡萄孢菌接种试验 作者:Jiajie Lian, Hongyu Han, Jiuhai Zhao and Chuanyou Li日期:04/20/2018,浏览量:10378,Q&A: 0
Botrytis cinerea (B. cinerea) attacks many crops of economic importance, represents one of the most extensively studied necrotrophic pathogens. Inoculation of B. cinerea and phenotypic analysis of plant resistance are key procedures to investigate the mechanism of plant immunity. Here we describe a protocol for B. ...
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