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生物化学

Isothermal Titration Calorimetry: A Biophysical Method to Characterize the Interaction between Label-free Biomolecules in Solution 等温滴定量热法:一种检测溶液中非标记生物分子相互作用的生物物理方法 作者:Andrea Saponaro日期:08/05/2018,浏览量:18182,Q&A: 0
This protocol can be applied to analyze the direct interaction between a soluble protein and a target ligand molecule using Isothermal Titration Calorimetry (ITC, Malvern). ITC allows the biophysical characterization of binding between label-free, non-immobilized and in-solution biomolecules by providing the stoichiometry of the interaction, the ...
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Inositol Phosphates Purification Using Titanium Dioxide Beads 利用二氧化钛珠纯化磷酸肌醇 作者:Miranda SC Wilson and Adolfo Saiardi日期:08/05/2018,浏览量:3888,Q&A: 0
Inositol phosphates (IPs) comprise a family of ubiquitous eukaryotic signaling molecules. They have been linked to the regulation of a pleiotropy of important cellular activities, but low abundance and detection difficulties have hampered our understanding. Here we present a method to purify and enrich IPs or other phosphate-rich metabolites from ...
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癌症生物学

Hypoxia Reporter Element Assay 低氧反应元件的检测分析 作者:Daelynn R Buelow and Sharyn D. D Baker日期:08/05/2018,浏览量:4587,Q&A: 0
Hypoxia is a condition in which there is a decrease in oxygen supply to the cellular environment. Changes to cellular oxygen levels can lead to transcriptional changes of oxygen-regulated genes. Reporter assays are used to study gene expression alteration and modifications in response to environmental changes. Dual-reporter assays allow the ...
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发育生物学

Isolation of LYVE-1+ Endothelial Cells from Mouse Embryos 从小鼠胚胎中分离LYVE-1+内皮细胞的方法 作者:Patrick Crosswhite日期:08/05/2018,浏览量:4038,Q&A: 0
Lymphatic vessel endothelial hyaluronan receptor 1, or LYVE-1, is a type 1 integral membrane glycoprotein expressed by lymphatic endothelial cells (LECs). LYVE-1 is commonly used as a biological marker to visually distinguish developing lymphatic vessels from blood endothelial cells (arteries or veins). As our understanding of lymphatic biology is ...
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微生物学

Long-term in vitro Culture of Cryptosporidium parvum 长期体外培养小隐孢子虫 作者:Nigel Yarlett and Mary Morada日期:08/05/2018,浏览量:5740,Q&A: 0
Continuous in vitro growth of Cryptosporidium parvum has proved difficult and conventional in vitro culture techniques result in short-term (2-5 days) growth of the parasite resulting in thin-walled oocysts that fail to propagate using in vitro cultures, and do not produce an active infection using ...
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Detection of Apoptosis-like Cell Death in Ustilago maydis by Annexin V-FITC Staining 膜联蛋白V-FITC染色检测玉米黑粉菌凋亡样细胞死亡 作者:Dibya Mukherjee, Aroni Mitra and Anupama Ghosh日期:08/05/2018,浏览量:4554,Q&A: 0
Programmed cell death (PCD) guides the transition between key developmental stages in many organisms. PCD also remains an important fate for many organisms upon exposure to different stress conditions. Therefore, an insight into the progression of PCD during the execution of a biological phenomenon can yield significant details of the underlying ...
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HCV Reporter System (Viral Infection-Activated Split-Intein-Mediated Reporter System) for Testing Virus Cell-to-cell Transmission ex-vivo 用于测试病毒细胞间传递的HCV报告系统(病毒感染-激活的断裂内含肽介导的报告系统) 作者:Fanfan Zhao, Ting Zhao, Libin Deng, Dawei Lv, Xiaolong Zhang, Xiaoyu Pan, Jun Xu and Gang Long日期:08/05/2018,浏览量:4093,Q&A: 0
Hepatitis C virus (HCV) spread involves two distinct entry pathways: cell-free transmission and cell-to-cell transmission. Cell-to-cell transmission is not only an efficient way for viruses to spread but also an effective method for escaping neutralizing antibodies. We adapted the viral infection-activated split-intein-mediated reporter system ...
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Structural Analysis of Bordetella pertussis Biofilms by Confocal Laser Scanning Microscopy 利用激光扫描共聚焦显微镜研究百日咳杆菌生物膜的结构 作者:Natalia Cattelan, Osvaldo Miguel Yantorno and Rajendar Deora日期:08/05/2018,浏览量:4543,Q&A: 0
Biofilms are sessile communities of microbial cells embedded in a self-produced or host-derived exopolymeric matrix. Biofilms can both be beneficial or detrimental depending on the surface. Compared to their planktonic counterparts, biofilm cells display enhanced resistance to killing by environmental threats, chemicals, antimicrobials and host ...
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Direct Visualization of the Multicopy Chromosomes in Cyanobacterium Synechococcus elongatus PCC 7942 蓝藻Synechococcus elongatus PCC 7942中多拷贝染色体的直接可视化观察 作者:Ryudo Ohbayashi, Hirofumi Yoshikawa and Satoru Watanabe日期:08/05/2018,浏览量:4808,Q&A: 0
Cyanobacteria are prokaryotic organisms that carry out oxygenic photosynthesis. The fresh water cyanobacterium Synechococcus elongatus PCC 7942 is a model organism for the study of photosynthesis and gene regulation, and for biotechnological applications. Besides several freshwater cyanobacteria, S. elongatus 7942 also contains ...
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Fusarium graminearum Inoculation on Wheat Head 禾谷镰刀菌的麦穗接种 作者:Chanjing Feng, Huiquan Liu and Zhe Tang日期:08/05/2018,浏览量:5926,Q&A: 0
Fusarium graminearum, the major causal agent of Fusarium head blight (FHB), causes serious wheat yield losses and a threat to human and animal health. The main efforts to combat the disease are the research of pathogenesis mechanisms and breeding for disease resistance plants. The efficiency of these actions could be evaluated by ...
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Determination of Storage (Starch/Glycogen) and Total Saccharides Content in Algae and Cyanobacteria by a Phenol-Sulfuric Acid Method 苯酚-硫酸法测定藻类和蓝藻中淀粉/糖原的储存和总糖含量 作者:Tomáš Zavřel, Petra Očenášová, Maria A. Sinetova and Jan Červený日期:08/05/2018,浏览量:7160,Q&A: 0
This is a protocol for quantitative determination of storage and total carbohydrates in algae and cyanobacteria. The protocol is simple, fast and sensitive and it requires only few standard chemicals. Great advantage of this protocol is that both storage and total saccharides can be determined in the cellular pellets that were already used for ...
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神经科学

Quantitative Electron Microscopic Assay Using Random Sampling from Single Sections to Test Plastic Synaptic Changes in Hippocampus 单切片随机取样定量电子显微技术检测海马区突触可塑性的变化 作者:G. Mark Marcello, Lilla E. Szabó, Peter Sotonyi and Bence Rácz日期:08/05/2018,浏览量:4238,Q&A: 0
Studies over several decades on the organization of the CA1 hippocampus–a particularly favorable model for learning, memory and certain forms of cognition–have shown that the synaptic network in this brain region is plastic (Fortin et al., 2012). Recent evidence suggests that a number of environmental and endogenous stimuli may have a ...
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植物科学

Arabidopsis-Green Peach Aphid Interaction: Rearing the Insect, No-choice and Fecundity Assays, and Electrical Penetration Graph Technique to Study Insect Feeding Behavior 拟南芥与绿桃蚜相互作用:昆虫喂养、无选择性育性分析和昆虫刺吸电位技术在研究昆虫摄食行为中的应用 作者:Vamsi Nalam, Joe Louis, Monika Patel and Jyoti Shah日期:08/05/2018,浏览量:6906,Q&A: 1
Aphids constitute a large group of Hemipterans that use their slender stylets to tap into the sieve elements of plants from which they consume copious amounts of phloem sap, thus depriving the plant of photoassimilates. Some aphids also transmit viral diseases of plants. Myzus persicae Sülzer, commonly known as the green peach aphid ...
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Measuring Secretion of Capsidiol in Leaf Tissues of Nicotiana benthamiana 烟草叶组织分泌辣椒素的测定 作者:Teruhiko Kuroyanagi, Maurizio Camagna and Daigo Takemoto日期:08/05/2018,浏览量:3769,Q&A: 0
Plant species produce a wide variety of antimicrobial metabolites to protect themselves against potential pathogens in natural environments. Phytoalexins are low molecular weight compounds produced by plants in response to attempted attacks of pathogens. Accumulation of phytoalexins in attacked plant tissues can inhibit the growth of penetrating ...
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Tethered Chromosome Conformation Capture Sequencing in Triticeae: A Valuable Tool for Genome Assembly 小麦族系中染色体构象捕获测序:一个有价值的基因组组装工具 作者:Axel Himmelbach, Ines Walde, Martin Mascher and Nils Stein日期:08/05/2018,浏览量:6315,Q&A: 0
Chromosome conformation capture sequencing (Hi-C) is a powerful method to comprehensively interrogate the three-dimensional positioning of chromatin in the nucleus. The development of Hi-C can be traced back to successive increases in the resolution and throughput of chromosome conformation capture (3C) (Dekker et al., 2002). The basic ...
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