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Image Credit: BioProtoc.2967


Modifying Styrene-maleic Acid Co-polymer for Studying Lipid Nanodiscs by Direct Fluorescent Labeling 改良的苯乙烯-马来酸共聚物通过直接荧光标记用于脂质纳米盘的研究 作者:Victoria Schmidt and James N. Sturgis日期:08/20/2018,浏览量:4505,Q&A: 0
This protocol was developed to functionalize styrene maleic acid (SMA) by direct fluorescent labeling in an easy way, accessible to biochemistry laboratories. This novel method is based on the coupling of carboxylic acids to primary amines using a carbodiimide, a reaction commonly used for protein chemistry. The procedure uses the hydrolyzed ...
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Deoxycholate Fractionation of Fibronectin (FN) and Biotinylation Assay to Measure Recycled FN Fibrils in Epithelial Cells 在上皮细胞中利用脱氧胆酸盐分离纤维粘连蛋白以及对回收的纤维粘连蛋白原纤维的生物素化分析 作者:Archana Varadaraj, Carina Magdaleno and Karthikeyan Mythreye日期:08/20/2018,浏览量:4043,Q&A: 0
Fibronectin (FN) is an extracellular matrix protein that is secreted by many cell types and binds predominantly to the cell surface receptor Integrin α5β1. Integrin α5β1 binding initiates the step-wise assembly of FN into fibrils, a process called fibrillogenesis. We and several others have demonstrated critical effects of fibrillogenesis on cell ...
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Identification and Quantitation of Leukocyte Populations in Human Kidney Tissue by Multi-parameter Flow Cytometry 利用多参数流式细胞技术对人肾脏组织中白细胞种群的鉴定与定量分析 作者:Katrina Kildey, Becker M.P. Law, Kimberly A. Muczynski, Ray Wilkinson, Helen Healy and Andrew J. Kassianos日期:08/20/2018,浏览量:6214,Q&A: 0
Inflammatory immune cells play direct pathological roles in cases of acute kidney injury (AKI) and chronic kidney disease (CKD). However, the identification and characterization of distinct populations of leukocytes in human kidney biopsies have been confounded by the limitations of immunohistochemical (IHC)-based techniques used to detect them. ...
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Microfluidics-Based Analysis of Contact-dependent Bacterial Interactions 基于微射流技术分析接触性依赖细菌相互作用 作者:Robert Cooper, Lev Tsimring and Jeff Hasty日期:08/20/2018,浏览量:4749,Q&A: 0
Bacteria in nature live in complex communities with multiple cell types and spatially-dependent interactions. Studying cells in well-mixed environments such as shaking culture tubes or flasks cannot capture these spatial dynamics, but cells growing in full-fledged biofilms are difficult to observe in real time. We present here a protocol for ...
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Assessment of Caenorhabditis elegans Competitive Fitness in the Presence of a Bacterial Parasite 寄生菌存在情况下秀丽隐杆线虫的竞争适应性测定 作者:McKenna. J. Penley and Levi T. Morran日期:08/20/2018,浏览量:4038,Q&A: 0
Accurate measurements of an organism’s fitness are crucial for measuring evolutionary change. Methods of fitness measurement are most accurate when incorporating an individual’s survival and fecundity, as well as accounting for any ecological interactions or environmental effects experienced by the organism. Here, we describe a protocol for ...
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In vivo and in vitro 31P-NMR Study of the Phosphate Transport and Polyphosphate Metabolism in Hebeloma cylindrosporum in Response to Plant Roots Signals 利用31P-NMR技术体内外研究柱孢白桦在响应植物根组织信号后的磷酸盐转运和多聚磷酸盐代谢 作者:Christine Le Guernevé, Adeline Becquer, Margarita Torres-Aquino, Laurie K Amenc, Carlos Trives-Segura, Siobhan Staunton, Claude Plassard and Hervé Quiquampoix日期:08/20/2018,浏览量:3897,Q&A: 0
We used in vivo and in vitro phosphorus-31 nuclear magnetic resonance (31P-NMR) spectroscopy to follow the change in transport, compartmentation and metabolism of phosphate in the ectomycorrhizal fungus Hebeloma cylindrosporum in response to root signals originating from host (Pinus pinaster) or ...
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Delivery of the Cas9 or TevCas9 System into Phaeodactylum tricornutum via Conjugation of Plasmids from a Bacterial Donor 通过细菌供体质粒接合转移的方法将Cas9或 TevCas9系统导入三角褐指藻 作者:Helen Wang, Samuel S. Slattery, Bogumil J. Karas and David R. Edgell日期:08/20/2018,浏览量:5461,Q&A: 2
Diatoms are an ecologically important group of eukaryotic microalgae with properties that make them attractive for biotechnological applications such as biofuels, foods, cosmetics and pharmaceuticals. Phaeodactylum tricornutum is a model diatom with defined culture conditions, but routine genetic manipulations are hindered by a lack of ...
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Random Insertional Mutagenesis of a Serotype 2 Dengue Virus Clone 2型登革病毒克隆的随机插入诱变 作者:Jeffrey W. Perry and Andrew W. Tai日期:08/20/2018,浏览量:3742,Q&A: 0
Protein tagging is a powerful method of investigating protein function. However, modifying positive-strand RNA virus proteins in the context of viral infection can be particularly difficult as their compact genomes and multifunctional proteins mean even small changes can inactivate or attenuate the virus. Although targeted approaches to ...
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CRISPR/Cas Gene Editing of a Large DNA Virus: African Swine Fever Virus 一种大DNA病毒(非洲猪瘟病毒)的CRISPR/Cas基因编辑 作者:Manuel V. Borca, Keith A. Berggren, Elizabeth Ramirez-Medina, Elizabeth A. Vuono and Douglas P. Gladue日期:08/20/2018,浏览量:6159,Q&A: 0
Gene editing of large DNA viruses, such as African swine fever virus (ASFV), has traditionally relied on homologous recombination of a donor plasmid consisting of a reporter cassette with surrounding homologous viral DNA. However, this homologous recombination resulting in the desired modified virus is a rare event. We recently reported the use of ...
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Liposome Flotation Assay for Studying Interactions Between Rubella Virus Particles and Lipid Membranes 用于风疹病毒颗粒和脂膜相互作用研究的脂质体浮选分析法 作者:Kyoko Saito, Noriyuki Otsuki, Makoto Takeda and Kentaro Hanada日期:08/20/2018,浏览量:6629,Q&A: 0
Rubella virus (RuV) is an enveloped, positive-sense single-stranded RNA virus that is pathogenic to humans. RuV binds to the target cell via the viral envelope protein E1, but the specific receptor molecules on the target cell are yet to be fully elucidated. Here, we describe a protocol for liposome flotation assay to study direct interactions ...
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Image-Based Analysis of Mitochondrial Area and Counting from Adult Mouse Dopaminergic Neurites 基于图像分析成熟小鼠多巴胺能神经元突起中线粒体的面积和数量 作者:Nadee Nissanka, Carlos T Moraes and Mlena Pinto日期:08/20/2018,浏览量:6428,Q&A: 0
Mitochondria form dynamic cytoplasmic networks which undergo morphological changes in order to adapt to cellular stresses and signals. These changes can include alterations in size and number within a given cell. Analysis of the whole network can be a useful metric to assess overall mitochondrial health, particularly in neurons, which are highly ...
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Trace Fear Conditioning: Procedure for Assessing Complex Hippocampal Function in Mice 痕迹条件性恐惧:小鼠中检测海马复杂性功能的步骤 作者:Vijendra Sharma, Noah Cohen, Rapita Sood, Hadile Ounallah-Saad, Shunit Gal-Ben-Ari and Kobi Rosenblum日期:08/20/2018,浏览量:6533,Q&A: 0
The trace fear conditioning protocol is designed to measure hippocampal function in mice. The protocol includes a neutral conditioned stimulus (tone) and an aversive unconditioned stimulus (shock), separated in time by a trace interval. The trace interval between the tone and the shock critically involves the hippocampus and could be used to ...
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Studying the Role of Microglia in Neurodegeneration and Axonal Regeneration in the Murine Visual System 小胶质细胞在小鼠视觉系统神经退变和轴突再生中的作用研究 作者:Alexander M Hilla and Dietmar Fischer日期:08/20/2018,浏览量:7817,Q&A: 0
Microglia reside in the central nervous system (CNS) and are involved in the maintenance of the physiologic state. They constantly survey their environment for pathologic alterations associated with injury or diseases. For decades, researchers have investigated the role of microglia under different pathologic conditions, using approaches aiming to ...
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An Inexpensive and Comprehensive Method to Examine and Quantify Field Insect Community Influenced by Host Plant Olfactory Cues 一种分析量化寄主植物嗅觉引诱性物质对田间昆虫群落影响的低成本、综合方法 作者:Rupesh Kariyat, Jesus Chavana and Jasleen Kaur日期:08/20/2018,浏览量:4076,Q&A: 0
Insect pollinators, herbivores and their natural enemies use olfactory cues emitted by their host plants to locate them. In insect-plant ecology, understanding the mechanisms underlying these interactions are of critical importance, as this bio-communication has both ecological and agricultural applications. However, the first step in such ...
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Pollen Germination and Pollen Tube Growth of Arabidopsis thaliana: In vitro and Semi in vivo Methods 体外及半体内实验方法研究拟南芥花粉发芽及花粉管的生长 作者:Hugh Dickinson, Josefina Rodriguez-Enriquez and Robert Grant-Downton 日期:08/20/2018,浏览量:11695,Q&A: 2
Studies of pollen germination and post-germination development are not only essential for understanding plant reproduction but also are an excellent model system for tip-based growth. Here we describe easy, reproducible methods for germination and growth of pollen from the model plant Arabidopsis thaliana in artificial conditions. Our ...
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Generation of Human Mesenchymal Stem Cell 3D Spheroids Using Low-binding Plates 利用低结合培养板培养产生人间充质干细胞3D球状体 作者:Elena Redondo-Castro, Catriona J Cunningham, Jonjo Miller, Stuart A Cain, Stuart M Allan and Emmanuel Pinteaux日期:08/20/2018,浏览量:6533,Q&A: 0
The 3D culture of human mesenchymal stem cells (hMSCs) represents a more physiological environment than classical 2D culture and has been used to enhance the MSC secretome or extend cell survival after transplantation. Here we describe a simple and affordable method to generate 3D spheroids of hMSCs by seeding them at high density in a low-binding ...
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Quantitative ChIP-seq by Adding Spike-in from Another Species 通过引入另一物种的标准参照以定量分析ChIP-seq 作者:Kongyan Niu, Rui Liu and Nan Liu日期:08/20/2018,浏览量:17611,Q&A: 0
Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a routine procedure in the lab; however, epigenome-wide quantitative comparison among independent ChIP-seq experiments remains a challenge. Here, we contribute an experimental protocol combined with a computational workflow allowing quantitative and comparative assessment of ...
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