• 卷册 11, 2021
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Image Credit: BioProtoc.3036

生物化学

Preparation of Sequencing RNA Libraries through Chemical Cross-linking Coupled to Affinity Purification (cCLAP) in Saccharomyces cerevisiae 通过化学交联联合亲和纯化(cCLAP) 技术制备酿酒酵母RNA测序文库 作者:Congwei Wang, Julie Weidner and Anne Spang日期:10/05/2018,浏览量:4378,Q&A: 0
Ribonucleoprotein particles (mRNPs) are complexes consisting of mRNAs and RNA-binding proteins (RBPs) which control mRNA transcription localization, turnover, and translation. Some mRNAs within the mRNPs have been shown to undergo degradation or storage. Those transcripts can lack general mRNA elements, like the poly(A) tail or 5’ cap structure, ...
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Expression and Ni-NTA-Agarose Purification of Recombinant Hepatitis C Virus E2 Ectodomain Produced in a Baculovirus Expression System 在杆状病毒表达系统中重组丙型肝炎病毒E2胞外结构域的表达和Ni-NTA-琼脂糖纯化 作者:Julián Gómez-Gutiérrez, Mar Rodríguez-Rodríguez, Francisco Gavilanes and Belén Yélamos日期:10/05/2018,浏览量:3839,Q&A: 0
In this protocol, we describe the production and purification of the ectodomain of the E2661 envelope protein (amino acids 384-661) of the Hepatitis C virus, which plays a fundamental role in the entry of the virus into the host cell. This protein has been expressed in both prokaryotic and eukaryotic systems but in small quantities or ...
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A Method for SUMO Modification of Proteins in vitro 体外蛋白SUMO化修饰的方法 作者:Christine C. Lee, Bing Li, Hongtao Yu and Michael J. Matunis日期:10/05/2018,浏览量:4532,Q&A: 0
The Small Ubiquitin-related Modifier (SUMO) is a protein that is post-translationally added to and reversibly removed from other proteins in eukaryotic cells. SUMO and enzymes of the SUMO pathway are well conserved from yeast to humans and SUMO modification regulates a variety of essential cellular processes including transcription, chromatin ...
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Filter Retardation Assay for Detecting and Quantifying Polyglutamine Aggregates Using Caenorhabditis elegans Lysates 使用秀丽隐杆线虫裂解液检测和定量聚谷氨酰胺聚集体的滤器延迟试验 作者:Olga Sin, Alejandro Mata-Cabana, Renée I. Seinstra and Ellen A. A. Nollen日期:10/05/2018,浏览量:5954,Q&A: 0
Protein aggregation is a hallmark of several neurodegenerative diseases and is associated with impaired protein homeostasis. This imbalance is caused by the loss of the protein’s native conformation, which ultimately results in its aggregation or abnormal localization within the cell. Using a C. elegans model of polyglutamine diseases, we ...
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细胞生物学

Maintenance of Schmidtea mediterranea in the Laboratory 实验室真涡虫的保存 作者:Nídia de Sousa and Teresa Adell日期:10/05/2018,浏览量:4584,Q&A: 0
In the last years, planarians have emerged as a unique model animal for studying regeneration and stem cells biology. Although their remarkable regenerative abilities are known for a long time, only recently the molecular tools to understand the biology of planarian stem cells and the fundamentals of their regenerative process have been ...
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发育生物学

Detection of Cell Death in Planarians 真涡虫细胞死亡的检测 作者:Nídia de Sousa and Teresa Adell日期:10/05/2018,浏览量:4525,Q&A: 0
Planarians are freshwater flatworms, well known for their ability to regenerate a complete organism from any piece of their body. Furthermore, planarians are constantly growing and degrowing throughout their lives, maintaining a functional and proportioned body. These properties rely on the presence of a population of adult stem cells and on the ...
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免疫学

Isolation and Culture of Mouse Lung ILC2s 小鼠肺ILC2s的分离和培养 作者:Pauline Schmitt, Anais Duval, Emilie Mirey, Jean-Philippe Girard and Corinne Cayrol日期:10/05/2018,浏览量:6087,Q&A: 0
Group 2 Innate Lymphoid Cells (ILC2) play an important role in immune responses at barrier surfaces, notably in the lung during airway allergic inflammation or asthma. Several studies have described methods to isolate ILC2s from wild-type naive mice, most of them using cell sorting to obtain a pure population. Here, we describe in detail, a ...
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微生物学

CRISPR/Cas9-mediated ssDNA Recombineering in Corynebacterium glutamicum 谷氨酸棒状杆菌中CRISPR/Cas9介导的ssDNA重组工程技术 作者:Jiao Liu, Yu Wang, Ping Zheng and Jibin Sun日期:10/05/2018,浏览量:4796,Q&A: 0
Corynebacterium glutamicum is a versatile workhorse for industrial bioproduction of many kinds of chemicals and fuels, notably amino acids. Development of advanced genetic engineering tools is urgently demanded for systems metabolic engineering of C. glutamicum. Recently unveiled clustered regularly interspaced short palindromic ...
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分子生物学

Fluorescence Titrations to Determine the Binding Affinity of Cyclic Nucleotides to SthK Ion Channels 荧光滴定法测定环核苷酸与SthK离子通道的结合亲和力 作者:Philipp A.M. Schmidpeter and Crina M. Nimigean日期:10/05/2018,浏览量:6434,Q&A: 0
The cyclic-nucleotide modulated ion channel family includes cyclic nucleotide-gated (CNG) and hyperpolarization-activated and cyclic nucleotide-modulated (HCN) channels, which play essential roles in visual and olfactory signaling and the heart pacemaking activity. Functionally, these channels have been extensively characterized by ...
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神经科学

Measurement of Dopamine Using Fast Scan Cyclic Voltammetry in Rodent Brain Slices 利用快速扫描循环伏安法检测啮齿动物大脑组织切片中的多巴胺 作者:Madelyn I. Mauterer, Paige M. Estave, Katherine M. Holleran and Sara R. Jones日期:10/05/2018,浏览量:7534,Q&A: 1
Fast scan cyclic voltammetry (FSCV) is an electrochemical technique that allows sub-second detection of oxidizable chemical species, including monoamine neurotransmitters such as dopamine, norepinephrine, and serotonin. This technique has been used to record the physiological dynamics of these neurotransmitters in brain tissue, including their ...
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Isolation of Chromatin-bound Proteins from Subcellular Fractions for Biochemical Analysis 从亚细胞成分中分离用于生化分析的染色质结合蛋白 作者:Sébastien Gillotin日期:10/05/2018,浏览量:13993,Q&A: 5
Shuttling of proteins between different cellular compartments controls their proteostasis and can contribute in some cases to regulate their activity. Biochemical analysis of chromatin-bound proteins, such as transcription factors, is often difficult because of their low yield and due to the interference from nucleic acids. This protocol describes ...
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Drosophila Endurance Training and Assessment of Its Effects on Systemic Adaptations 果蝇耐力训练及其对系统适应性影响的评估 作者:Deena Damschroder , Tyler Cobb, Alyson Sujkowski and Robert Wessells日期:10/05/2018,浏览量:4669,Q&A: 0
Exercise induces beneficial systemic adaptations that reduce the incidence of age-related diseases. However, the molecular pathways that elicit these adaptations are not well understood. Understanding the molecular mechanisms that underlie the exercise response can lead to widely beneficial therapies. Large populations, relatively short lifespan, ...
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植物科学

Real-time PCR Analysis of PAMP-induced Marker Gene Expression in Nicotiana benthamiana 本塞姆氏烟草中PAMP诱导标记基因表达的实时定量PCR分析 作者:Fan Liu, Yuanpeng Xu, Yan Wang and Yuanchao Wang日期:10/05/2018,浏览量:5558,Q&A: 0
Perception of pathogen-associated molecular patterns (PAMPs) often triggers various innate immune responses in plants. The transcriptional changes of defense-related genes are often used as a marker to assay PAMP-triggered plant immune response. Here we described a protocol to monitor the relative expression level of marker genes in Nicotiana ...
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An Image Analysis Pipeline to Quantify Emerging Cracks in Materials or Adhesion Defects in Living Tissues 分析活体组织中出现的材料裂缝或附着力缺陷的图像分析法 作者:Stéphane Verger, Guillaume Cerutti and Olivier Hamant日期:10/05/2018,浏览量:4213,Q&A: 0
Microcracks in materials reflect their mechanical properties. The quantification of the number or orientation of such cracks is thus essential in many fields, including engineering and geology. In biology, cracks in soft tissues can reflect adhesion defects, and the analysis of their pattern can help to deduce the magnitude and orientation of ...
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