• 卷册 11, 2021
  • 卷册 10, 2020
  • 卷册 9, 2019
  • 卷册 8, 2018
  • 卷册 7, 2017
  • 卷册 6, 2016
  • 卷册 5, 2015
  • 卷册 4, 2014
  • 卷册 3, 2013
  • 卷册 2, 2012
  • 卷册 1, 2011
Image Credit: BioProtoc.3110


Wheat Germ Agglutinin (WGA)-SDS-PAGE: A Novel Method for the Detection of O-GlcNAc-modified Proteins by Lectin Affinity Gel Electrophoresis 小麦种子凝集素(WGA)-SDS-PAGE:一种利用凝集素亲和凝胶电泳对O-乙酰氨基葡萄糖修饰蛋白进行检测的新方法 作者:Ko Fujioka, Yuji Kubota and Mutsuhiro Takekawa日期:12/05/2018,浏览量:5381,Q&A: 0
Diverse cytoplasmic and nuclear proteins dynamically change their molecular functions by O-linked β-N-acetylglucosamine (O-GlcNAc) modification on serine and/or threonine residues. Evaluation of the O-GlcNAcylation level of a specific protein, however, needs multiple and time-consuming steps if using conventional methods (e.g., ...
更多 >>
Purification of Globular Actin from Rabbit Muscle and Pyrene Fluorescent Assays to Investigate Actin Dynamics in vitro 从兔肌肉组织中纯化球状肌动蛋白并利用芘荧光分析实验体外检测肌动蛋白的动态变化 作者:He Sun, Yuanyuan Luo and Yansong Miao日期:12/05/2018,浏览量:3854,Q&A: 0
Pyrene Fluorescent Assay is established to monitor the dynamic actin nucleation, elongation, capping and disassembly in vitro. This technique provides an easy handle procedure and straightforward visual data analysis. By coupling actin purification and polymerization assays in this protocol, the readers could quickly get the affordable ...
更多 >>
In vitro Membrane Interaction and Liposome Fusion Assays Using Recombinant Hepatitis C Virus Envelope Protein E2 利用重组丙型肝炎病毒包膜蛋白E2体外检测膜互作和脂质体融合 作者:Belén Yélamos, Mar Rodríguez-Rodríguez, Francisco Gavilanes and Julián Gómez-Gutiérrez日期:12/05/2018,浏览量:3061,Q&A: 0
In order to study the mechanism underlying the Hepatitis C Virus (HCV) fusion process we have performed assays using phospholipid liposomes and a truncated form of E2 protein, E2661 (amino acids 384-661 of the HCV polyprotein) lacking the transmembrane region. E2661 has been previously generated by using the baculovirus ...
更多 >>


Cluster Analysis of Endogenous HER2 and HER3 Receptors in SKBR3 Cells 在SKBR3细胞中内源性HER2和HER3受体的聚类分析 作者:Selene K. Roberts, Michael Hirsch, Alexandra McStea, Laura C. Zanetti-Domingues, David T. Clarke, Jeroen Claus, Peter J. Parker, Lin Wang and Marisa L. Martin-Fernande日期:12/05/2018,浏览量:3641,Q&A: 0
The Human Epidermal Growth Factor Receptor (HER) family of receptor tyrosine kinases consists of four, single pass, transmembrane receptor homologs (HER1-4) that act to regulate many critical processes in normal and tumor cells. HER2 is overexpressed in many tumors, and the deregulated proliferation of cancerous cells is driven by cooperation with ...
更多 >>


Blinded Visual Scoring of Images Using the Freely-available Software Blinder 使用免费软件Blinder对图像进行盲视觉评分 作者:Steven D. Cothren, Joel N. Meyer and Jessica H. Hartman日期:12/05/2018,浏览量:3894,Q&A: 0
In nearly all subfields of biomedical sciences, there are phenotypes that are currently classified by expert visual scoring. In research applications, these classifications require the experimenter to be blinded to the treatment group in order to avoid unintentional bias in scoring. Currently, many labs either use laborious and tedious methods to ...
更多 >>


Notochord Injury Assays that Stimulate Transcriptional Responses in Zebrafish Larvae 诱导幼年期斑马鱼转录响应的脊索损伤实验 作者:Zhiqiang Zeng, Juan C Lopez-Baez, Laura Lleras-Forero, Hannah Brunsdon, Cameron Wyatt, Witold Rybski, Nicholas D Hastie, Stefan Schulte-Merker and E Elizabeth Patton日期:12/05/2018,浏览量:3467,Q&A: 0
Zebrafish have become an increasingly important model organism in the field of wound healing and regenerative medicine, due to their high regenerative capacity coupled with high-resolution imaging in living animals. In a recent study, we described multiple physical and chemical methods to induce notochord injury that led to highly specific ...
更多 >>


Flow Cytometry Assay for Recycling of LFA-1 in T-lymphocytes 流式细胞术检测T淋巴细胞中LFA-1的再循环 作者:Katarzyna Potrzebowska, Janne Lehtonen, Malin Samuelsson and Lena Svensson日期:12/05/2018,浏览量:3335,Q&A: 0
To enable cells to move forward, cell surface integrins are internalized into an endosomal compartment and subsequently intracellularly transported to be re-exposed at a new site on the cell membrane. Leukocytes are the fastest migrating cell type in the human body, which express the leukocyte-specific integrin LFA-1. Here, we describe a flow ...
更多 >>
Integrin Dependent RhoB Activation Assay Using Leukocytes 白细胞内整合素依赖的RhoB激活实验 作者:Janne Lehtonen, Katarzyna Potrzebowska, Malin Samuelsson and Lena Svensson日期:12/05/2018,浏览量:1844,Q&A: 0
To be able to migrate, leukocyte needs to re-use its adhesion molecules to move forward. These adhesion molecules are called integrins and are intracellularly transported via endocytosis and exocytosis in order to translocate to a new site on the cell membrane. The intracellular transportation is regulated by different small GTPases including ...
更多 >>
Ultrasound Guided Intra-thymic Injection to Track Recent Thymic Emigrants and Investigate T Cell Development 超声引导胸腺内注射并追踪新近胸腺迁出细胞和T细胞发育观测 作者:Haiguang Wang, David L. Owen, Lily J. Qian, Laura B. Chopp, Michael A. Farrar and Kristin A. Hogquist日期:12/05/2018,浏览量:3145,Q&A: 0
To track recent thymic emigrants (RTEs) or study T cell development in the thymus, intra-thymic injection of a cellular tag or precursor cells for various T cell lineages is often desired. However, the traditional surgical approach to expose the thymus for intra-thymic injection is time-consuming and can cause a high level of pain and stress to ...
更多 >>


Field Inoculation and Classification of Maize Ear Rot Caused by Fusarium verticillioides 田间接种和轮枝镰孢菌引起的玉米穗粒腐病的分类 作者:Chaopei Dong, Yabin Wu, Jingyang Gao, Zijian Zhou, Cong Mu, Peipei Ma, Jiafa Chen and Jianyu Wu日期:12/05/2018,浏览量:4518,Q&A: 0
Maize ear rot is a worldwide fungal disease mainly caused by Fusarium verticillioides and Fusarium graminearum. Maize planted in the field was inoculated with Fusarium verticillioides at the filling stage, 15 days after pollination. Two milliliters of spore suspension with a concentration of 5 x 106/ml was ...
更多 >>
Quantitative Transformation Efficiency Assay for Bacillus subtilis 枯草芽孢杆菌转化效率的定量测定 作者:Christian L. Loyo and Briana M. Burton日期:12/05/2018,浏览量:5562,Q&A: 0
Bacillus subtilis (B. subtilis) is a model Gram-positive organism used to study a variety of physiological states and stress responses, one of which is the development of competence. Competence refers to the physiological state of a cell which allows it to be transformed naturally. Through induction of competence, the efficiency ...
更多 >>
An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated Protein 利用芽殖酵母微管相关蛋白研究微管结合和微管交联的体外显微镜检测法 作者:Yili Zhu, Weimin Tan and Wei-Lih Lee日期:12/05/2018,浏览量:4541,Q&A: 0
In this protocol, we describe a simple microscopy-based method to assess the interaction of a microtubule-associated protein (MAP) with microtubules. The interaction between MAP and microtubules is typically assessed by a co-sedimentation assay, which measures the amount of MAP that co-pellets with microtubules by centrifugation, followed by ...
更多 >>


Genome-wide Estimation of Evolutionary Distance and Phylogenetic Analysis of Homologous Genes 进化距离的全基因组评估和同源基因的系统进化分析 作者:Meixia Zhao, Biao Zhang, Jianxin Ma and Damon Lisch日期:12/05/2018,浏览量:5060,Q&A: 0
Homologous genes, including paralogs and orthologs, are genes that share sequence homologies within or between different species. Homologous genes originate from a common origin through speciation, genetic duplication or horizontal gene transfer. Estimation of the sequence divergence of homologous genes help us to understand divergence time, which ...
更多 >>
Using Arabidopsis Mesophyll Protoplasts to Study Unfolded Protein Response Signaling 利用拟南芥叶肉细胞原生质体研究未折叠蛋白响应信号 作者:Yan Bao and Diane C. Bassham日期:12/05/2018,浏览量:4052,Q&A: 0
Various environmental stresses or artificial reagents can trigger unfolded protein accumulation in the endoplasmic reticulum (ER) due to the folding capacity of the ER being exceeded. This is termed ER stress, and triggers the unfolded protein response (UPR). Assays for activation of the UPR in plants include Tunicamycin (Tm)- or dithiothreitol ...
更多 >>