• 卷册 11, 2021
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Image Credit: BioProtoc.3115

生物化学

Preparation of Silk Fibroin Nanoparticles and Enzyme-Entrapped Silk Fibroin Nanoparticles 丝素蛋白纳米颗粒和酶包裹丝素蛋白纳米颗粒 作者:Yu-Qing Zhang日期:12/20/2018,浏览量:5568,Q&A: 0
After silk fiber is degummed in boiling 0.2% Na2CO3 solution, the degummed fibroin fiber could be dissolved in highly concentrated neutral salts such as CaCl2. The partially degraded polypeptides of silk fibroin, commonly called as regenerated liquid silk, could be obtained via water dialysis. The silk fibroin ...
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细胞生物学

Quantitative Analysis of Cargo Density in Single-extracellular Vesicles by Imaging 成像技术定量分析单细胞外囊泡中内容物的密度 作者:Taketoshi Kajimoto and Shun-ichi Nakamura日期:12/20/2018,浏览量:3926,Q&A: 0
Function of extracellular vesicles such as exosomes and microvesicles is determined by their wide ranges of cargoes inside them. Even in the pure exosomes or microvesicles the cargo contents are very heterogeneous. To understand this heterogeneous nature of extracellular vesicles, we need information of the vesicles, which will give us some ...
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Microirradiation for Precise, Double-strand Break Induction in vivo in Caenorhabditis elegans 显微照射用于秀丽隐杆线虫中双链断裂的精确体外诱导 作者:Kailey E. Harrell, Emily Koury and Sarit Smolikove日期:12/20/2018,浏览量:3296,Q&A: 0
DNA double-strand breaks (DSBs) are toxic lesions that every cell must accurately repair in order to survive. The repair of DSBs is an integral part of a cell life cycle and can lead to lethality if repaired incorrectly. Laser microirradiation is an established technique which has been used in yeast, mammalian cell culture, and Drosophila ...
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免疫学

Quantitation of Regulatory Activity for the Complement Alternative Pathway Using an Adaptation of the AP50 in vitro Assay 应用改编过的AP50体外分析法定量测定补体旁路途径的调节活性 作者:Sheila Cabezas Falcon, David L Gordon and Jillian M Carr日期:12/20/2018,浏览量:3734,Q&A: 2
Complement pathways function to identify and remove pathogens and infected cells. There are three complement pathways: the classical, lectin and alternative pathway (AP). While all pathways are activated following pathogen stimuli, the AP is constitutively active and tightly controlled by activators (e.g., Factor B, Factor D) and negative ...
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Eimeria vermiformis Infection Model of Murine Small Intestine 蠕形艾美耳球虫对小鼠小肠的感染模型 作者:Patrícia Figueiredo-Campos, Cristina Ferreira, Birte Blankenhaus and Marc Veldhoen日期:12/20/2018,浏览量:3493,Q&A: 0
Eimeria vermiformis is a tissue specific, intracellular protozoan that infects the murine small intestinal epithelia, which has been widely used as a coccidian model to study mucosal immunology. This mouse infection model is valuable to investigate the mechanisms of host protection against primary and secondary infection in the small ...
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Method for Studying the Effect of Gene Silencing on Bacterial Infection-induced ERK1/2 Signaling in Bone-marrow Derived Macrophages 基因沉默对于细菌感染诱导骨髓巨噬细胞中ERK1/2信号通路的影响研究方法 作者:Gaurav Kumar, Subhash B. Arya and Amit Tuli日期:12/20/2018,浏览量:4145,Q&A: 0
Macrophages are highly phagocytic cells that utilize various pathogen recognition receptors (PRRs) to recognize pathogen-associated molecular patterns (PAMPs). These PAMPs can be present within the microbe, such as bacterial CpG DNA, and are recognized by Toll-like receptor 9 (TLR9), a PRR present on the endosomal membrane of macrophages. PAMPs ...
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Monitoring Natural Killer Cell Function in Human Ovarian Cancer Cells of Ascitic Fluid 在人卵巢腹水癌细胞中监测自然杀手细胞功能 作者:Vikash Kumar and Asok Mukhopadhyay日期:12/20/2018,浏览量:3857,Q&A: 0
Natural killer (NK) cells are the major effectors of the innate immune system when activated resulting in modulation of immune response of the host defense through target cell lysis and secretion of cytokines. Precise functions of NK cells are essential for the treatment outcome of different virus infections and malignant diseases. NK cells impart ...
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微生物学

Production, Titration and Imaging of Zika Virus in Mammalian Cells 哺乳动物细胞中寨卡病毒的制备、滴度测定和成像 作者:Wesley Freppel, Clément Mazeaud and Laurent Chatel-Chaix日期:12/20/2018,浏览量:7683,Q&A: 0
Since the outbreak of Zika virus (ZIKV) in Latin America and the US in 2016, this flavivirus has emerged as a major threat for public health. Indeed, it is now clear that ZIKV is vertically transmitted from the infected mother to the fetus and this may lead to severe neurological development defects including (but not restricted to) neonate ...
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Systematic Quantification of GFP-tagged Protein Foci in Schizosaccharomyces pombe Nuclei 粟酒裂殖酵母细胞核中GFP标记蛋白聚集点的系统量化 作者:Kim Kiat Lim and Ee Sin Chen日期:12/20/2018,浏览量:3656,Q&A: 0
DNA damage repair proteins form foci in response to DNA damaging agents. The efficiency and integrity of the DNA repair pathway of a particular eukaryotic (mutant) strain is usually determined by the number of foci formed compared with their wild-type counterpart. Conventionally, focus number is determined visually, and this low accuracy may ...
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Analysis of DNA Exchange Using Thymidine Analogs (ADExTA) in Trypanosoma cruzi 克氏锥虫中利用胸苷类似物进行DNA交换分析 作者:Marcelo S. da Silva, Paula A. Marin, Bruno M. Repolês, Maria C. Elias and Carlos R. Machado日期:12/20/2018,浏览量:2872,Q&A: 0
Trypanosoma cruzi is a protozoan parasite belonging to the Trypanosomatidae family. Although the trypanosomatids multiply predominantly by clonal generation, the presence of DNA exchange in some of them has been puzzling researchers over the years, mainly because it may represent a novel form that these organisms use to gain variability. ...
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分子生物学

Variable Dose Analysis: A Novel High-throughput RNAi Screening Method for Drosophila Cells 可变剂量分析:一种果蝇细胞高通量RNAi筛选的新方法 作者:Katarzyna Sierzputowska, Chris R. Baxter and Benjamin E. Housden日期:12/20/2018,浏览量:3133,Q&A: 0
Genetic screens are a powerful approach to identify previously uncharacterized genes involved in specific biological processes. Several technologies have been developed for high-throughput screens using reagents such as RNAi or CRISPR, and each approach is associated with specific advantages and disadvantages. Variable Dose Analysis (VDA), is an ...
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神经科学

Partial Transection of Adult Rat Optic Nerve as a Model of Secondary Degeneration in the Central Nervous System 成年大鼠视神经不完全横切作为中枢神经系统继发性损害模型 作者:Carole A Bartlett and Melinda Fitzgerald日期:12/20/2018,浏览量:2890,Q&A: 0
Injury to the central nervous system is characterized by damage that spreads from the initial point of impact into the surrounding adjacent tissue, in a phenomenon referred to as secondary degeneration. The optic nerve can be used to effectively model injury and secondary degeneration to white matter tracts. Partial transection of the dorsal ...
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Three-chamber Social Approach Task with Optogenetic Stimulation (Mice) 配有光遗传学刺激的三间隔社交途径测试(小鼠) 作者:Celeste Leung, Jun Chul Kim and Zhengping Jia日期:12/20/2018,浏览量:4755,Q&A: 0
The formation of social relationships via social interactions and memory are essential for one’s physical and mental health. To date, rodent studies have used the three-chamber social approach test to measure social approach, social novelty, and social memory. In recent years, techniques including optogenetics have been developed to acutely ...
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植物科学

Visualization and Quantification of Cell-to-cell Movement of Proteins in Nicotiana benthamiana 本氏烟中蛋白的细胞间运动的可视化定量检测 作者:Mila C Blekemolen, Mariliis Tark-Dame and Frank L.W Takken日期:12/20/2018,浏览量:4614,Q&A: 0
Cell-to-cell movement of proteins through plasmodesmata is a widely-established mechanism for intercellular signaling in plants. Current techniques to study intercellular protein translocation rely on single-cell transformation using particle bombardment or transgenic lines expressing photo-inducible fluorophores. The method presented here allows ...
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