• 卷册 11, 2021
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Image Credit: BioProtoc.3309

生物化学

Isoelectric Focusing to Quantify Rhodopsin Phosphorylation in Mouse Retina 等电聚焦用于小鼠视网膜中磷酸化视紫红质的定量分析 作者:Sowmya Bekshe Lokappa, M. Carter Cornwall and Jeannie Chen日期:07/20/2019,浏览量:4220,Q&A: 0
Rhodopsin is a G-protein coupled receptor (GPCR) that mediates vision under dim light. Upon light exposure, rhodopsin is phosphorylated at multiple serine and threonine sites at its carboxyl-terminus by rhodopsin kinase (GRK1). This, in turn, reduces its ability to activate the visual G-protein transducin. Binding of light-activated, ...
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In vitro RNA Cleavage Assays to Characterize IRE1-dependent RNA Decay 通过体外RNA剪切检测IRE1依赖的RNA降解 作者:G. Elif Karagöz, Jirka Peschek, Peter Walter and Diego Acosta-Alvear日期:07/20/2019,浏览量:4672,Q&A: 0
The kinase/RNase IRE1 is a key effector of the cellular response to endoplasmic reticulum stress. The RNase activity of IRE1 can be measured in cells or in the test tube. Here we describe a protocol for the in vitro cleavage and analysis of RNA substrates of IRE1. The method consists of the in vitro transcription, purification ...
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癌症生物学

Total RNA Isolation from Separately Established Monolayer and Hydrogel Cultures of Human Glioblastoma Cell Line 单层水凝胶培养人胶质母细胞瘤细胞系的全RNA分离 作者:Manasi P Jogalekar and Elba E Serrano日期:07/20/2019,浏览量:3595,Q&A: 0
Astrocytoma is an invasive carcinoma occurring in the nervous system and currently lacks effective treatment options. A deeper understanding of the mechanisms of tumorigenesis and tumor progression is needed in order to develop novel therapeutic strategies. Recent advances in in vitro culture systems have demonstrated that the use of ...
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Evaluation of Genotoxicity by Micronucleus Assay in vitro and by Allium cepa Test in vivo 利用微核体外分析系统和洋葱体内实验进行遗传毒性测试 作者:Christina N. Banti and Sotiris K. Hadjikakou日期:07/20/2019,浏览量:5321,Q&A: 0
The in vitro and in vivo genotoxicity of new metallodrugs either as Small Bioactive Molecules (SBAMs) or Conjugates of Metals with Drugs (CoMeDs) is evaluated by the micronucleus test and the Allium cepa assay, respectively. Fetal lung fibroblast cells (MRC-5), normal human corneal epithelial cells (HCEC) and ...
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细胞生物学

Electron Microscopy Sample Preparation Protocol Enabling Nano-to-mesoscopic Mapping of Cellular Connectomes and Their Habitats in Human Tissues and Organs 通过电镜样品制备实现的人体组织和器官中细胞连接体及其定位的纳米与介观图谱分析 作者:Lucy Ngo, Anton D. Nathanson, Tomasz Garbowski, Ulf Knothe, Dirk Zeidler and Melissa L. Knothe Tate日期:07/20/2019,浏览量:4777,Q&A: 0
Multibeam scanning electron microscopy (multiSEM) provides a technical platform for seamless nano-to-mesoscale mapping of cells in human tissues and organs, which is a major new initiative of the U.S. National Institutes of Health. Such cross-length-scale imaging is expected to provide unprecedented understanding of relationships between cellular ...
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Lipid-exchange Rate Assay for Lipid Droplet Fusion in Live Cells 活体细胞中脂滴融合的脂类交换速率检测 作者:Jia Wang, Boon Tin Chua, Peng Li and Feng-Jung Chen日期:07/20/2019,浏览量:3832,Q&A: 0
Lipid droplets (LDs) are central organelles in maintaining lipid homeostasis. Defective LD growth often results in the development of metabolic disorders. LD fusion and growth mediated by cell death–inducing DNA fragmentation factor alpha (DFFA)-like effector (CIDE) family proteins are crucial for various biological processes including unilocular ...
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免疫学

Isolation and Long-term Cultivation of Mouse Alveolar Macrophages 小鼠肺泡巨噬细胞的分离与长期培养 作者:Clara Jana-Lui Busch, Jérémy Favret, Laufey Geirsdóttir, Kaaweh Molawi and Michael H. Sieweke日期:07/20/2019,浏览量:6317,Q&A: 0
Alveolar macrophages (AM) are tissue-resident macrophages that colonize the lung around birth and can self-maintain long-term in an adult organism without contribution of monocytes. AM are located in the pulmonary alveoli and can be harvested by washing the lungs using the method of bronchoalveolar lavage (BAL). Here, we compared different ...
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微生物学

A Protocol to Map the Spatial Proteome Using HyperLOPIT in Saccharomyces cerevisiae 利用HyperLOPIT绘制酿酒酵母空间蛋白质组图谱的方法 作者:Daniel J. H. Nightingale, Kathryn S. Lilley and Stephen G. Oliver日期:07/20/2019,浏览量:3612,Q&A: 0
The correct subcellular localization of proteins is vital for cellular function and the study of this process at the systems level will therefore enrich our understanding of the roles of proteins within the cell. Multiple methods are available for the study of protein subcellular localization, including fluorescence microscopy, organelle ...
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神经科学

A Standardized Tank Design for the Light Dark Task in Zebrafish 一种用于探究斑马鱼明暗节律的培养箱标准设计 作者:Amanda Facciol, Steven Tran and Robert Gerlai日期:07/20/2019,浏览量:3059,Q&A: 0
The light dark paradigm is a common behavioral test used to screen a variety of pharmacological agents, including anxiogenics and anxiolytics. Although most often used in rodents, the light dark task has recently been adapted for use in zebrafish. However, a number of inconsistent findings have been reported for this species. Some have found ...
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Protocol for Measuring Compulsive-like Feeding Behavior in Mice 小鼠强迫性进食行为的检测步骤 作者:Marta Fructuoso, Jose Espinosa-Carrasco, Ionas Erb, Cedric Notredame and Mara Dierssen日期:07/20/2019,浏览量:3284,Q&A: 0
Obesity is an important health problem with a strong environmental component that is acquiring pandemic proportion. The high availability of caloric dense foods promotes overeating potentially causing obesity. Animal models are key to validate novel therapeutic strategies, but researchers must carefully select the appropriate model to draw the ...
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Axon-seq for in Depth Analysis of the RNA Content of Neuronal Processes 神经元突起RNA含量的Axon-seq 深入分析 作者:Jik Nijssen, Julio Aguila and Eva Hedlund日期:07/20/2019,浏览量:8762,Q&A: 0
Neuronal processes have an RNA composition that is distinct from the cell body. Therefore, to fully understand neuronal biology in health and disease we need to study both somas, dendrites and axons. Here we describe a detailed protocol of a newly refined method, Axon-seq, for RNA sequencing of axons (and dendrites) grown in isolation using single ...
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植物科学

Isolation of Powdery Mildew Haustoria from Infected Barley 感染大麦中白粉病吸器的分离提取 作者:Linhan Li, Benjamin Collier and Pietro D. Spanu日期:07/20/2019,浏览量:4415,Q&A: 0
Blumeria graminis is a fungus that causes powdery mildews on grasses, such as barley. Investigations of this pathogen present many challenges due to its obligate biotrophic nature. This means that the fungus can only grow in the presence of a living host plant. B. graminis forms epiphytic mycelia on the plant surface and feeding ...
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Quantification of Blumenol Derivatives as Leaf Biomarkers for Plant-AMF Association 蓝甲醇衍生物作为丛枝菌根真菌与植物共生标志物的定量研究 作者:Elisabeth Mindt, Ming Wang, Martin Schäfer, Rayko Halitschke and Ian T. Baldwin日期:07/20/2019,浏览量:3668,Q&A: 0
Symbiotic interactions between arbuscular mycorrhizal fungi (AMF) and plants are widespread among land plants and can be beneficial for both partners. The plant is provided with mineral nutrients such as nitrogen and phosphorous, whereas it provides carbon resources for the fungus in return. Due to the large economic and environmental impact, ...
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干细胞

Isolation and Culture of Single Myofiber and Immunostaining of Satellite Cells from Adult C57BL/6J Mice 成年C57BL/6J大鼠单个肌纤维的培养分离和卫星细胞的免疫染色 作者:Shujie Chen, Hongrong Ding, Xiangping Yao and Liwei Xie日期:07/20/2019,浏览量:4939,Q&A: 0
Myofiber isolation followed with ex vivo culture could recapitulate and visualize satellite cells (SCs) activation, proliferation, and differentiation. This approach could be taken to understand the physiology of satellite cells and the molecular mechanism of regulatory factors, in terms of the involvement of intrinsic factors over SCs ...
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