• 卷册 11, 2021
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癌症生物学

Metabolic Assays for Detection of Neutral Fat Stores 代谢试验检测中性脂肪储存 作者:Jan M. Baumann, Leila Kokabee, Xianhiu Wang, Yan Sun, Jason Wong and Douglas S. Conklin日期:06/20/2015,浏览量:12906,Q&A: 1
Lipid droplets (LDs) are ubiquitous intracellular structures whose formation, growth, and maintenance are highly regulated (Wang et al., 2013; Ranall et al., 2011; Goodman, 2009). Lipid metabolism and droplet dynamics are of considerable interest to agriculture, biofuel production, viral pathology, nutrition, and cancer biology ...
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免疫学

Ex vivo Human Natural Killer (NK) Cell Stimulation and Intracellular IFNγ and CD107a Cytokine Staining 人自然杀伤细胞(NK)的细胞体外刺激和 IFNγ 及CD107a 细胞因子胞内染色 作者:Vanessa A. York and Jeffrey M. Milush日期:06/20/2015,浏览量:14798,Q&A: 1
Natural killer (NK) cells comprise 5–20% of peripheral blood mononuclear cells (PBMC) in humans. In addition to their fundamental roles in the defense against viral infections and tumor surveillance, NK cells help shape adaptive immune responses through their production of cytokines. NK cells are traditionally identified as CD3neg, CD14 ...
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Non-invasive Intratracheal Instillation in Mice 小鼠无创气管滴注法 作者:Guadalupe Ortiz-Muñoz and Mark R. Looney日期:06/20/2015,浏览量:15345,Q&A: 1
The intratracheal instillation technique is used to deliver a variety of agents to the lungs ranging from pathogens (bacteria, viruses), toxins, to therapeutic agents. To model lung inflammation and injury, LPS can be administrated via intranasal, intratracheal, or aerosol approaches. Each technique has its limitations. The intratracheal technique ...
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Two-event Transfusion-related Acute Lung Injury Mouse Model 通过双重免疫刺激建立输血相关急性肺损伤的小鼠模型 作者:Guadalupe Ortiz-Muñoz and Mark R. Looney日期:06/20/2015,浏览量:7406,Q&A: 0
Transfusion-related acute lung injury (TRALI) is defined as acute lung injury that occurs within 6 hours of a blood product transfusion. TRALI continues to be a leading cause of transfusion-related mortality and we have developed a mouse model of TRALI to better understand the mechanisms by which injury occurs and to test therapeutic approaches. ...
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微生物学

Generating Isogenic Deletions (Knockouts) in Francisella tularensis, a Highly-infectious and Fastidious Gram-negative Bacterium 在高传染性和需要多种养分的革兰氏阴性菌-土拉弗朗西斯菌敲除突变株的获得方法 作者:Xiaojun Wu, Guoping Ren and Jason F. Huntley日期:06/20/2015,浏览量:8241,Q&A: 0
Generating bacterial gene deletion mutants, also known as knockouts (KOs), is a powerful tool to investigate individual gene functions. However, fastidious bacteria such as Francisella tularensis (F. tularensis) often are difficult to genetically manipulate. Indeed, many different approaches have been tested to generate F. ...
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Visual Assessment of the Severity of Fusarium Seedling Blight (FSB) and Fusarium Head Blight (FHB) Disease in Barley 大麦中镰刀菌属苗枯病(FSB)和镰刀菌属赤霉病(FHB)严重性的目测评估 作者:Shahin S. Ali, Lokanadha R. Gunupuru and Fiona M. Doohan日期:06/20/2015,浏览量:8288,Q&A: 0
Fusarium pathogens are among the most damaging pathogens of cereals. These pathogens have the ability to attack the roots, seedlings, and flowering heads of barley and wheat plants (Simpson et al., 2004). Resulting in yield loss and head blight disease and also resulting in the contamination of grain with mycotoxins harmful to ...
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Mismatched Primer Extension Assays 错配引物延伸法 作者:Vasudevan Achuthan and Jeffrey J. DeStefano日期:06/20/2015,浏览量:6312,Q&A: 0
Steady state kinetic assays have been a reliable way to estimate fidelity of several polymerases (Menendez-Arias, 2009; Rezende and Prasad, 2004; Svarovskaia et al., 2003). The ability to analyze the extension of primers with specific mismatches at the 3ʹ end is a major strength of the mismatched primer extension assays. Recently, we used ...
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Primer Extension Reactions for the PCR- based α- complementation Assay 引物延伸反应用于基于PCR的α-互补试验 作者:Vasudevan Achuthan and Jeffrey J. DeStefano日期:06/20/2015,浏览量:9053,Q&A: 0
The PCR- based- α- complementation assay is an effective technique to measure the fidelity of polymerases, especially RNA-dependent RNA polymerases (RDRP) and Reverse Transcriptases (RT). It has been successfully employed to determine the fidelity of the poliovirus polymerase 3D-pol (DeStefano, 2010) as well as the human immunodeficiency virus ...
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神经科学

Electroporation of Embryonic Chick Eyes 鸡胚胎视网膜DNA电转移 作者:Agustín Luz-Madrigal, Erika Grajales-Esquivel and Katia Del Rio-Tsonis日期:06/20/2015,浏览量:11349,Q&A: 0
The chick embryo has prevailed as one of the major models to study developmental biology, cell biology and regeneration. From all the anatomical features of the chick embryo, the eye is one of the most studied. In the chick embryo, the eye develops between 26 and 33 h after incubation (Stages 8-9, Hamburger and Hamilton, 1951). It originates from ...
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植物科学

Gentiobiose Feeding in Gentian in vitro Overwintering Buds or Plantlets 在龙胆越冬芽及越冬苗上体外施加龙胆二糖 作者:Hideyuki Takahashi and Masahiro Nishihara日期:06/20/2015,浏览量:6573,Q&A: 0
To study the functions of sugars in plants, feeding experiment is one of the most common and easy methods. However, the traditional method, e.g., a floating of leaf discs on sugar-containing solution seems to have an insufficient efficiency of sugar uptake, despite of high osmotic and injury effects. This is a protocol to feed ...
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A Simple Protocol for the Immunolabelling of Arabidopsis Pollen Tube Membranes and Cell Wall Polymers 拟南芥花粉管膜和细胞壁聚合物的简单免疫标记法 作者:Marie Dumont, Céline Cataye, Arnaud Lehner, Eric Maréchal, Patrice Lerouge, Denis Falconet and Jean-Claude Mollet日期:06/20/2015,浏览量:9375,Q&A: 0
The pollen tube, a fast tip-growing cell, is an excellent model to study membrane and cell wall biosynthesis. Here, we describe a simple protocol using an easy to use device to perform immunofluorescence labelling of pollen tube membrane and cell wall. The use of the NucleoSpin column to perform all the steps of the immunolabelling procedure ...
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Analysis of Sugar Component of a Hot Water Extract from Arabidopsis thaliana Pollen Tubes Using GC-EI-MS 采用 GC-EI-MS分析拟南芥花粉管中热水提取物的糖组分 作者:Marie Dumont, Arnaud Lehner, Corinne Loutelier-Bourhis, Jean-Claude Mollet and Patrice Lerouge日期:06/20/2015,浏览量:6948,Q&A: 0
Extraction with hot water is the oldest and simplest method used to recover pectin from an alcohol insoluble residue extract, although this method has not been widely used for the cell wall analysis of pollen tube, a model used to study cell wall. This protocol described this method applied for pectin extraction from 6 h-old Arabidopsis ...
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Virus-induced Gene Silencing (VIGS) in Barley Seedling Leaves 大麦幼苗叶的病毒诱导基因沉默(VIGS) 作者:Lokanadha R. Gunupuru, Shahin S. Ali, Fiona M. Doohan and Steven R. Scofield日期:06/20/2015,浏览量:10665,Q&A: 2
Virus induced gene silencing (VIGS) is one of the most potent reverse genetics technologies for gene functional characterisation. This method exploits a dsRNA-mediated antiviral defence mechanism in plants. Using this method allows researchers to generate rapid phenotypic data in a relatively rapid time frame as compared to the generation of ...
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Development and Implementation of an in vitro Culture System for Intact Detached Grape Berries 完整分离的葡萄果实体外培养系统的开发与实现 作者:Zhanwu Dai, Messa Meddar, Serge Delrot and Eric Gomès日期:06/20/2015,浏览量:9549,Q&A: 0
Grape composition depends on the metabolites accumulated and synthesized during grape development. It is of paramount importance for grape growers because of its major role in shaping wine quality. Therefore, understanding the regulation mechanisms that control the accumulation of quality-related metabolites in grape is of both scientific and ...
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