• 卷册 10, 2020
  • 卷册 9, 2019
  • 卷册 8, 2018
  • 卷册 7, 2017
  • 卷册 6, 2016
  • 卷册 5, 2015
  • 卷册 4, 2014
  • 卷册 3, 2013
  • 卷册 2, 2012
  • 卷册 1, 2011
Image Credit: BioProtoc.3506

往期刊物(2020)

卷册: 10 期号: 3

生物物理学

Evaluating Whole Blood Clotting in vitro on Biomaterial Surfaces 生物材料表面进行全血凝固的体外评估 作者:Roberta M Sabino and Ketul C Popat日期:02/05/2020,浏览量:1767,Q&A: 0
Biomaterial-associated thrombosis is still a major concern for blood-contacting implants. After the medical device is implanted and comes in contact with blood, several complex reactions occur, which may lead to thrombus formation and failure of the device. Therefore, it is essential to evaluate the biomaterial interaction with the whole blood. ...
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癌症生物学

Approaching RNA-seq for Cell Line Identification RNA序列在细胞系鉴定中的应用 作者:Tabrez A. Mohammad and Yidong Chen日期:02/05/2020,浏览量:1474,Q&A: 0
Cancer cell lines serve as invaluable model systems for cancer biology research and help in evaluating the efficacy of new therapeutic agents. However, cell line contamination and misidentification have become one of the most pressing problems affecting biomedical research. Available methods of cell line authentication suffer from limited access, ...
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Detection of Individual RNA in Fixed Cells and Tissues by Chromogenic ISH 利用显色原位杂交进行固定细胞和组织中单个RNA的检测 作者:Meng Jiang, Chen Lin and Rongqin Ke日期:02/05/2020,浏览量:1372,Q&A: 0
Visualization of RNA molecules in situ helps to better understand the functions of expressed genes. Currently, most conventional in situ hybridization methods for visualization of individual RNAs are based on fluorescence detection. Herein we present a chromogenic in situ hybridization protocol for visualization of ...
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Measurement of ATPase Activity of Valosin-containing Protein/p97 含缬酪肽蛋白/p97的ATP酶活性检测 作者:Kruthi Suvarna, Kaori Honda, Makoto Muroi, Yasumitsu Kondoh, Hiroyuki Osada and Nobumoto Watanabe日期:02/05/2020,浏览量:1675,Q&A: 0
Valosin-containing protein (VCP; also known as p97) is a type II ATPase regulating several cellular processes. Using proteomic techniques, we identified a chemical compound that binds to the D1 ATPase domain of VCP. The protocol described here was to study the effect of the compound on ATPase activity in vitro of purified VCP protein. ...
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Identification of Target Protein for Bio-active Small Molecule Using Photo-cross Linked Beads and MALDI-TOF Mass Spectrometry 光交联微球和MALDI-TOF质谱法鉴定生物活性小分子靶蛋白 作者:Kruthi Suvarna, Kaori Honda, Makoto Muroi, Yasumitsu Kondoh, Nobumoto Watanabe and Hiroyuki Osada日期:02/05/2020,浏览量:1644,Q&A: 0
Development of methods for protein identification is one of the important aspects of proteomics. Here, we report a protocol for the preparation of compound conjugated beads by photo-crosslinking, affinity purification, gel electrophoresis, and highly sensitive mass spectrometric assay for drug-target identification. Although there are several ...
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细胞生物学

Live Mitochondrial or Cytosolic Calcium Imaging Using Genetically-encoded Cameleon Indicator in Mammalian Cells 利用基因编码Cameleon指示剂进行哺乳动物细胞中活体线粒体或胞质钙成像 作者:Elisa Greotti and Tullio Pozzan日期:02/05/2020,浏览量:2018,Q&A: 0
Calcium (Ca2+) imaging aims at investigating the dynamic changes in live cells of its concentration ([Ca2+]) in different pathophysiological conditions. Ca2+ is an ubiquitous and versatile intracellular signal that modulates a large variety of cellular functions thanks to a cell type-specific toolkit and a complex ...
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Rapid Nickel-based Isolation of Extracellular Vesicles from Different Biological Fluids 从不同的生物体液中进行胞外囊泡的快速镍基分离 作者:Michela Notarangelo, Deborah Ferrara, Cristina Potrich, Lorenzo Lunelli, Lia Vanzetti, Alessandro Provenzani, Manuela Basso, Alessandro Quattrone and Vito G. D'Agostino日期:02/05/2020,浏览量:1910,Q&A: 0
Extracellular vesicles (EVs) are membranous structures that cells massively release in extracellular fluids. EVs are cargo of cellular components such as lipids, proteins, and nucleic acids that can work as a formidable source in liquid biopsy studies searching for disease biomarkers. We recently demonstrated that nickel-based isolation (NBI) is a ...
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发育生物学

Mouse Embryonic Tooth Germ Dissection and Ex vivo Culture Protocol 小鼠胚胎牙胚分离和体外培养方案 作者:Xue Han, Keigo Yoshizaki, Tian Tian, Kanako Miyazaki, Ichiro Takahashi and Satoshi Fukumoto日期:02/05/2020,浏览量:1780,Q&A: 1
A tooth germ ex vivo organ culture allows visualization of its development in different stages, thus enabling investigation of the molecular mechanisms of regulatory factors. Tooth germs can be rapidly dissected from E13 mouse embryos and placed on cell culture inserts for observation of subsequent tooth germ development in a ...
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微生物学

Transcervical Mouse Infections with Chlamydia trachomatis and Determination of Bacterial Burden 小鼠宫颈沙眼衣原体感染及细菌负荷测定 作者:Karthika Rajeeve and Rajeeve Sivadasan日期:02/05/2020,浏览量:1684,Q&A: 0
Chlamydia trachomatis is an obligate human pathogen. It infects the genital tract of humans ascending into the fallopian tube, exacerbated by chronic pelvic pain, pelvic inflammatory disease, and fallopian tube scaring resulting in infertility and other malignancies. The major hurdle in controlling chlamydial spread is that the ...
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Rapid Detection of Proliferative Bacteria by Electrical Stimulation 利用电刺激快速检测细菌增殖 作者:Conor LA Edwards, Dmitry Malyshev, James P Stratford and Munehiro Asally日期:02/05/2020,浏览量:2139,Q&A: 0
Detecting live bacteria is an important task for antimicrobial susceptibility testing (AST) in the medical sector and for quality-monitoring in biological industries. Current methods for live-bacteria detection suffer limitations in speed or sensitivity. In a recent paper, we reported that electrical response dynamics in membrane potential enable ...
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Expression and Purification of Adeno-associated Virus Virus-like Particles in a Baculovirus System and AAVR Ectodomain Constructs in E. coli 腺相关病毒样颗粒在杆状病毒系统中的表达纯化及在大肠杆菌AAVR胞外区的构建 作者:Nancy Meyer, Omar Davulcu, Qing Xie, Mark Silveria, Grant M. Zane, Edward Large and Michael S. Chapman日期:02/05/2020,浏览量:1665,Q&A: 0
Adeno-associated virus (AAV) is a promising gene therapy vector and the biophysical characterization of its interactions with host proteins is a critical foundation for engineering tissue targeting and immune escape. Presented here are protocols for the production of: (a) the outer protein shells (virus-like particles or VLPs) for serotype 2 ...
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Competition Assays to Quantify the Effect of Biocontrol Yeasts against Plant Pathogenic Fungi on Fruits 竞争分析以量化酵母菌对水果上植物病原真菌的生防作用 作者:Electine Magoye, Melanie Pfister, Maja Hilber-Bodmer and Florian M. Freimoser日期:02/05/2020,浏览量:1203,Q&A: 0
Yeasts such as Aureobasidium pullulans are unicellular fungi that occur in all environments and play important roles in biotechnology, medicine, food and beverage production, research, and agriculture. In the latter, yeasts are explored as biocontrol agents for the control of plant pathogenic fungi (e.g., Botrytis cinerea, ...
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分子生物学

Structural Alignment and Covariation Analysis of RNA Sequences RNA序列的结构比对和协变分析 作者:Nicolas J. Tourasse and Fabien Darfeuille日期:02/05/2020,浏览量:1657,Q&A: 0
RNA molecules adopt defined structural conformations that are essential to exert their function. During the course of evolution, the structure of a given RNA can be maintained via compensatory base-pair changes that occur among covarying nucleotides in paired regions. Therefore, for comparative, structural, and evolutionary studies of RNA ...
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神经科学

Implanting and Recycling Neuropixels Probes for Recordings in Freely Moving Mice 自由运动小鼠 Neuropixels 探针的植入与回收 作者:Ashley L. Juavinett, George Bekheet and Anne K. Churchland日期:02/05/2020,浏览量:2331,Q&A: 0
Recording neural activity in unrestricted animals is necessary to unravel the neural basis of ethological behaviors. Recently, Neuropixels probes have made important strides in improving yield and lowering noise, but have limited use cases in freely moving animals. Although there are a number of studies demonstrating the use of these probes in ...
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干细胞

In vitro Self-organized Mouse Small Intestinal Epithelial Monolayer Protocol 小鼠小肠上皮单层膜体外自组装实验方案 作者:Gizem Altay, Eduard Batlle, Vanesa Fernández-Majada and Elena Martinez日期:02/05/2020,浏览量:3267,Q&A: 0
Developing protocols to obtain intestinal epithelial monolayers that recapitulate in vivo physiology to overcome the limitations of the organoids’ closed geometry has become of great interest during the last few years. Most of the developed culture models showed physiological-relevant cell composition but did not prove self-renewing ...
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