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Image Credit: BioProtoc.3524


Photoactivable Cholesterol as a Tool to Study Interaction of Influenza Virus Hemagglutinin with Cholesterol 利用光活化胆固醇研究流感病毒血凝素与胆固醇相互作用 作者:Bodan Hu, Mohamed Rasheed Gadalla, Christoph Thiele and Michael Veit日期:02/20/2020,浏览量:2418,Q&A: 0
Non-covalent binding of cholesterol to the transmembrane region of proteins affect their functionalities, but methods to prove such an interaction are rare. We describe our protocol to label the hemagglutinin (HA) of Influenza virus with a cholesterol derivative in living cells or with immunoprecipitated protein. We synthesized a “clickable” ...
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ChIP-Seq from Limited Starting Material of K562 Cells and Drosophila Neuroblasts Using Tagmentation Assisted Fragmentation Approach 用Tagmentation Assisted Fragmentation法从K562细胞和果蝇神经母细胞的有限起始材料中提取芯片序列 作者:Junaid Akhtar, Piyush More and Steffen Albrecht日期:02/20/2020,浏览量:2073,Q&A: 0
Chromatin immunoprecipitation is extensively used to investigate the epigenetic profile and transcription factor binding sites in the genome. However, when the starting material is limited, the conventional ChIP-Seq approach cannot be implemented. This protocol describes a method that can be used to generate the chromatin profiles from as low as ...
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CRISPR-Cas9 Genome Editing of Plasmodium knowlesi 诺氏疟原虫CRISPR-Cas9基因编辑 作者:Franziska Mohring, Melissa N. Hart, Avnish Patel, David A. Baker and Robert W. Moon日期:02/20/2020,浏览量:3268,Q&A: 1
Plasmodium knowlesi is a zoonotic malaria parasite in Southeast Asia that can cause severe and fatal malaria in humans. The main hosts are Macaques, but modern diagnostic tools reveal increasing numbers of human infections. After P. falciparum, P. knowlesi is the only other malaria parasite capable of being maintained in ...
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Estimating Cellular Abundances of Halo-tagged Proteins in Live Mammalian Cells by Flow Cytometry 利用流式细胞术进行哺乳动物活细胞中Halo 标记蛋白细胞丰度测定 作者:Claudia Cattoglio, Xavier Darzacq, Robert Tjian and Anders Sejr Hansen日期:02/20/2020,浏览量:3578,Q&A: 0
Accurate abundance measurements of cellular proteins are required to achieve a quantitative and predictive understanding of any biological process inside the cell. Existing methods to determine absolute protein abundances are labor-intensive and/or require sophisticated experimental and computational infrastructure (e.g., fluorescence ...
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Preparation of Single Epithelial Cells Suspension from Mouse Mammary Glands 小鼠乳腺单个上皮细胞悬液制备 作者:Heng Sun, Xiaoling Xu and Chuxia Deng日期:02/20/2020,浏览量:2499,Q&A: 0
Single cell RNA sequencing is a very powerful means for cellular heterogeneous studies and so becoming wildly utilized nowadays. To guarantee the success of such analysis, it is very important, though sometimes difficult, to obtain single cells suspension with high quality, especially from the primary solid organs like mammary glands. Digestion of ...
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RNA Sequencing of Single Myofibers from Mus musculus 小鼠单肌纤维的RNA序列分析 作者:Darren M. Blackburn, Felicia Lazure and Vahab D. Soleimani日期:02/20/2020,浏览量:3653,Q&A: 0
Whole transcriptome analysis is a key method in biology that allows researchers to determine the effect a condition has on gene regulation. One difficulty in RNA sequencing of muscle is that traditional methods are performed on the whole muscle, but this captures non-myogenic cells that are part of the muscle. In order to analyze only the ...
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Skin Transplantation and Lymphoid Organ Analysis in Mice 小鼠中皮肤移植和淋巴器官分析 作者:Julie Ruer-Laventie, Rajesh Jayachandran, Mathias Schmaler, Simona W. Rossi and Jean Pieters日期:02/20/2020,浏览量:2543,Q&A: 0
Skin transplantation in mice is an important procedure to evaluate immune responses generated against heterologous grafts, especially given its highly immunogenic nature. In fact, skin is one of the most challenging organs in terms of allograft retention. In this protocol, we provide a detailed procedure for skin grafting using the tail skin as ...
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Surface Plasmon Resonance Analysis of the Protein-protein Binding Specificity Using Autolab ESPIRIT 利用Autolab ESPIRIT进行表面等离子体共振分析蛋白间结合特异性 作者:Pragyan Parimita Rath, Gaurav Anand and Shalini Agarwal日期:02/20/2020,浏览量:3983,Q&A: 0
Direct protein-protein interactions are known to regulate a wide range of cellular activities. To understand these contacts one can employ various experimental methods like Dynamic Light Scattering (DLS), Fluorescence Resonance Energy Transfer (FRET), Isothermal titration calorimetry (ITC), Chemical crosslinking, Co-immunoprecipitation (Co-IP), ...
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Super-resolution Microscopy-based Bimolecular Fluorescence Complementation to Study Protein Complex Assembly and Co-localization 基于高分辨率显微镜的双分子荧光互补用于蛋白复合体装配和共定位 作者:Jingjing Chen, Zulin Yu, Jay R. Unruh, Brian D. Slaughter and Sue L. Jaspersen日期:02/20/2020,浏览量:2961,Q&A: 0
Numerous experimental approaches exist to study interactions between two subunits of a large macromolecular complex. However, most methods do not provide spatial and temporal information about binding, which are critical for dissecting the mechanism of assembly of nanosized complexes in vivo. While recent advances in super-resolution ...
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Assessing Self-interaction of Mammalian Nuclear Proteins by Co-immunoprecipitation 共免疫沉淀法评价哺乳动物核蛋白的自身相互作用 作者:Claudia Cattoglio, Iryna Pustova, Xavier Darzacq, Robert Tjian and Anders S. Hansen日期:02/20/2020,浏览量:3962,Q&A: 0
Protein-protein interactions constitute the molecular foundations of virtually all biological processes. Co-immunoprecipitation (CoIP) experiments are probably the most widely used method to probe both heterotypic and homotypic protein-protein interactions. Recent advances in super-resolution microscopy have revealed that several nuclear proteins ...
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Hippocampal Unicellular Recordings and Hippocampal-dependent Innate Behaviors in an Adolescent Mouse Model of Alzheimer’s disease 阿尔茨海默症幼鼠模型中海马单细胞记录和海马依赖先天性行为 作者:Siddhartha Mondragón-Rodríguez, Benito Ordaz, Erika Orta-Salazar, Sofia Díaz-Cintra, Fernando Peña-Ortega and George Perry日期:02/20/2020,浏览量:2001,Q&A: 0
Transgenic mice have been used to make valuable contributions to the field of neuroscience and model neurological diseases. The simultaneous functional analysis of hippocampal cell activity combined with hippocampal dependent innate task evaluations provides a reliable experimental approach to detect fine changes during early phases of ...
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Method for Primary Epithelial Cell Culture from the Rat Choroid Plexus 大鼠脉络丛原代上皮细胞培养方法 作者:Valeria Lallai, Amina Ahmed and Christie D. Fowler日期:02/20/2020,浏览量:2742,Q&A: 0
The choroid plexus consists of a network of secretory epithelial cells localized throughout the lateral, third and fourth ventricles of the brain. Cerebrospinal fluid (CSF) is generated by the choroid plexus and released into the ventricular environment. This biofluid contains an enriched source of proteins, ions, and other signaling molecules for ...
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Induction of Temporal Lobe Epilepsy in Mice with Pilocarpine 毛果芸香碱诱导小鼠颞叶癫痫 作者:Muhammad Nauman Arshad and Janice R Naegele日期:02/20/2020,浏览量:3241,Q&A: 0
In the pilocarpine model of temporal lobe epilepsy (TLE) in rodents, systemic injections of pilocarpine induce continuous, prolonged limbic seizures, a condition termed “Status Epilepticus” (SE). With appropriate doses, many inbred strains of mice show behavioral seizures within an hour after pilocarpine is injected. With the behavioral scoring ...
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Construction of Antisense RNA-mediated Gene Knock-down Strains in the Cyanobacterium Anabaena sp. PCC 7120 反义RNA介导的鱼腥藻PCC 7120基因敲除株的构建 作者:Amit Srivastava, Anand Ballal, Karl Forchhammer and Anil Kumar Tripathi日期:02/20/2020,浏览量:2836,Q&A: 0
Anabaena sp. PCC 7120 (hereafter Anabaena) is a model cyanobacterium to study nitrogen fixation, cellular differentiation and several other key biological functions that are analogous in plants. As with any other organism, many genes in Anabaena encode an essential life function and hence cannot be deleted, causing a ...
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Mesenchymal Stromal Cells Derived from Bone Marrow and Adipose Tissue: Isolation, Culture, Characterization and Differentiation 骨髓和脂肪间充质干细胞:分离、培养、鉴定和分化 作者:Gileade P. Freitas, Alann T. P. Souza, Helena B. Lopes, Rayana L. B. Trevisan, Fabiola S. Oliveira, Roger R. Fernandes, Fernanda U. Ferreira, Felipe A. Ros, Marcio M. Beloti and Adalberto L. Rosa日期:02/20/2020,浏览量:3940,Q&A: 1
Since their discovery, mesenchymal stromal cells (MSCs) have received a lot of attention, mainly due to their self-renewal potential and multilineage differentiation capacity. For these reasons, MSCs are a useful tool in cell biology and regenerative medicine. In this article, we describe protocols to isolate MSCs from bone marrow (BM-MSCs) and ...
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