• 卷册 10, 2020
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  • 卷册 4, 2014
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Image Credit: BioProtoc.3544


卷册: 10 期号: 5


Production, Purification and Characterization of Recombinant Biotinylated Phytochrome B for Extracellular Optogenetics 重组生物素化光敏色素B的制备、纯化、鉴定用于细胞外光遗传学研究 作者:Maximilian Hörner, O. Sascha Yousefi, Wolfgang W. A. Schamel and Wilfried Weber日期:03/05/2020,浏览量:1379,Q&A: 0
In the field of extracellular optogenetics, photoreceptors are applied outside of cells to obtain systems with a desired functionality. Among the diverse applied photoreceptors, phytochromes are the only ones that can be actively and reversibly switched between the active and inactive photostate by the illumination with cell-compatible red and ...
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Manganese Superoxide Dismutase Activity Assay in the Yeast Saccharomyces cerevisiae 啤酒酵母中的锰超氧化物歧化酶活性测定 作者:Louise Thines and Pierre Morsomme日期:03/05/2020,浏览量:1254,Q&A: 0
Superoxide dismutases (SODs) act as a primary defence against reactive oxygen species (ROS) by converting superoxide anion radicals (O2-) into molecular oxygen (O2) and hydrogen peroxide (H2O2). Members of this enzyme family include CuZnSODs, MnSODs, FeSODs, and NiSODs, depending on the nature ...
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Assembly of Genetic Circuits with the Mammalian ToolKit 用哺乳动物工具包组装遗传电路 作者:João P. Fonseca, Alain R Bonny, Jason Town and Hana El-Samad日期:03/05/2020,浏览量:1522,Q&A: 0
The ability to rapidly assemble and prototype cellular circuits is vital for biological research and its applications in biotechnology and medicine. The Mammalian ToolKit (MTK) is a Golden Gate-based cloning toolkit for fast, reproducible and versatile assembly of large DNA vectors and their implementation in mammalian models. The MTK consists of ...
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Lipid Mixing Assay for Murine Myoblast Fusion and Other Slow Cell-cell Fusion Processes 小鼠成肌细胞融合及其他慢细胞融合过程的脂质混合实验 作者:Evgenia Leikina, Kamran Melikov, Anthony G. Rabinovich, Douglas P. Millay and Leonid V. Chernomordik日期:03/05/2020,浏览量:1284,Q&A: 0
Lipid mixing (redistribution of lipid probes between fusing membranes) has been widely used to study early stages of relatively fast viral and intracellular fusion processes that take seconds to minutes. Lipid mixing assays are especially important for identification of hemifusion intermediates operationally defined as lipid mixing without content ...
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Zebrafish Bacterial Infection Assay to Study Host-Pathogen Interactions 斑马鱼细菌感染检测用于研究宿主-病原相互作用 作者:Faiza Basheer, Clifford Liongue and Alister C Ward日期:03/05/2020,浏览量:1307,Q&A: 0
The study of host-pathogen interactions has improved our understanding of both pathogenesis and the response of the host to infection, including both innate and adaptive responses. Neutrophils and macrophages represent the first line of innate host defense against any infection. The zebrafish is an ideal model to study the response of these cells ...
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Optogenetic Tuning of Ligand Binding to The Human T cell Receptor Using The opto-ligand-TCR System 利用光配体TCR系统调节配体与人T细胞受体的结合 作者:O. Sascha Yousefi, Maximilian Hörner, Maximilian Wess, Vincent Idstein, Wilfried Weber and Wolfgang W. A. Schamel日期:03/05/2020,浏览量:1557,Q&A: 0
T cells are one major cell type of the immune system that use their T cell antigen receptor (TCR) to bind and respond to foreign molecules derived from pathogens. The ligand-TCR interaction half-lives determine stimulation outcome. Until recently, scientists relied on mutating either the TCR or its ligands to investigate how varying TCR-ligand ...
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Growth Recovery Assay and FACS-based Population Sorting Following Territorial Exclusion in Proteus mirabilis 奇异变形杆菌被领土排斥后生长恢复测定和基于FACS的群体分类 作者:Murray J. Tipping and Karine A. Gibbs日期:03/05/2020,浏览量:1370,Q&A: 0
Many bacteria take part in self recognition and kin discrimination behavior using contact-dependent effectors. Understanding the effects these effectors cause is important to explain bacterial community formation and population dynamics. Typically, kin discrimination effectors are toxins that kill target cells; their effect is therefore obvious ...
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Contemporaneous Measurement of Outer and Inner Membrane Permeability in Gram-negative Bacteria 革兰阴性菌中内外膜通透性的同期测定 作者:Bo Ma, Chao Fang, Jing Zhang, Mingzhi Wang, Xiaoxing Luo and Zheng Hou日期:03/05/2020,浏览量:1278,Q&A: 0
The emergence and rapid spread of multidrug resistance in bacteria have led to the urgent need for novel antibacterial agents. Membrane permeabilization is the mechanism for many antibacterial molecules that are being developed against gram-negative bacteria. Thus, to determine the efficacy of a potential antibacterial molecule, it is important to ...
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Cell-free Reconstitution of the Packaging of Cargo Proteins into Vesicles at the trans Golgi Network 反面高尔基体网状结构中货物蛋白包装成囊泡的无细胞重组 作者:Xiao Tang, Feng Yang and Yusong Guo日期:03/05/2020,浏览量:1331,Q&A: 0
Protein sorting at the trans Golgi network (TGN) plays important roles in targeting newly synthesized proteins to their specific destinations. The aim of this proposal is to reconstitute the packaging of non-Golgi resident cargo proteins into vesicles at the TGN, utilizing rat liver cytosol, semi-intact mammalian cells and nucleotides. ...
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Delayed Alternation Task for the Study of Spatial Working and Long Term Memory in Rats 延迟交替任务用于研究大鼠空间工作记忆和长期记忆 作者:Megi Hoxha and Marta Sabariego日期:03/05/2020,浏览量:1559,Q&A: 0
Memory systems can hold previously presented information for several seconds, bridging gaps between discontinuous events. It has been previously demonstrated that the hippocampus and the medial entorhinal cortex (mEC) are necessary for memory retention over delay intervals in alternation tasks. Here we describe the delayed alternation task, a ...
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Tandem Tag Assay Optimized for Semi-automated in vivo Autophagic Activity Measurement in Arabidopsis thaliana roots 优化的串联标签检测用于拟南芥根组织中体内半自动化自噬活性测定 作者:Adrian N. Dauphinee, Jonas A. Ohlsson and Elena A. Minina日期:03/05/2020,浏览量:1483,Q&A: 0
Autophagy is the main catabolic process in eukaryotes and plays a key role in cell homeostasis. In vivo measurement of autophagic activity (flux) is a powerful tool for investigating the role of the pathway in organism development and stress responses. Here we describe a significant optimization of the tandem tag assay for detection of ...
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Insect Feeding Assays with Spodoptera exigua on Arabidopsis thaliana 甜菜夜蛾对拟南芥的取食试验 作者:Yanrong You, Chunpeng An and Chuanyou Li日期:03/05/2020,浏览量:1372,Q&A: 0
Plant-insect interaction is an important field for studying plant immunity. The beet armyworm, Spodoptera exigua, is one of the best-known agricultural pest insects and is usually used to study plant interactions with chewing insects. Here, we describe a protocol for insect feeding assays with Spodoptera exigua lavae using model ...
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Quantification of Protein Enrichment at Plasmodesmata 胞间连丝富集蛋白的量化分析 作者:Magali S. Grison, Jules D. Petit, Marie Glavier and Emmanuelle M. Bayer日期:03/05/2020,浏览量:1288,Q&A: 0
Intercellular communication plays a crucial role in the establishment of multicellular organisms by organizing and coordinating growth, development and defence responses. In plants, cell-to-cell communication takes place through nanometric membrane channels called plasmodesmata (PD). Understanding how PD dictate cellular connectivity greatly ...
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Single-run HPLC Quantification of Plant Cell Wall Monosaccharides Single-run HPLC定量分析植物细胞壁单糖 作者:Alexandra Menna, Michaela Fischer-Stettler, Barbara Pfister, Gloria Sancho Andrés, Duncan Holbrook-Smith and Clara Sánchez-Rodríguez日期:03/05/2020,浏览量:919,Q&A: 0
The plant cell wall is a complex network of polysaccharides and proteins that provides strength and structural integrity to plant cells, as well as playing a vital role in growth, development, and defense response. Cell wall polysaccharides can be broadly grouped into three categories: cellulose, pectins, and hemicelluloses. Dynamic interactions ...
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mRNA Extraction from Gill Tissue for RNA-sequencing 鳃组织mRNA 提取用于RNA测序 作者:Jukka-Pekka Verta and Felicity Jones日期:03/05/2020,浏览量:1336,Q&A: 0
Adaptation is thought to proceed in part through spatial and temporal changes in gene expression. Fish species such as the threespine stickleback are powerful vertebrate models to study the genetic architecture of adaptive changes in gene expression since divergent adaptation to different environments is common, they are abundant and easy to study ...
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