• 卷册 10, 2020
  • 卷册 9, 2019
  • 卷册 8, 2018
  • 卷册 7, 2017
  • 卷册 6, 2016
  • 卷册 5, 2015
  • 卷册 4, 2014
  • 卷册 3, 2013
  • 卷册 2, 2012
  • 卷册 1, 2011
Image Credit: BioProtoc.3573


卷册: 10 期号: 7


Preparation, FPLC Purification and LC-FT-ICR-MS of Proteins 蛋白质的制备、FPLC纯化及LC-FT-ICR-MS 作者:Tyler B. Johnson, Jiri Adamec and Paul Blum日期:04/05/2020,浏览量:1291,Q&A: 0
High magnetic field Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometers provide extremely high mass resolution (resolving power of ~200,000 at 400 m/z) protein detection across a broad mass range, enabling analysis of fine structure of isotopic peak clusters that is missed in other types of mass spectrometers. The protocol ...
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Determination of Flavin Potential in Proteins by Xanthine/Xanthine Oxidase Method 黄嘌呤/黄嘌呤氧化酶法测定蛋白质中的黄素电位 作者:Elena Maklashina and Gary Cecchini日期:04/05/2020,浏览量:1223,Q&A: 0
This protocol describes a simple xanthine/xanthine oxidase enzymatic equilibration method for determination of the redox potential of a flavin. As an example of the use of this method, we determine the reduction potential of the covalently bound FAD cofactor (Em = -55 mV) in the SdhA flavoprotein subunit of succinate ...
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Measurement of Protein-Protein Interactions through Microscale Thermophoresis (MST) 利用微量热泳动仪 (MST)检测蛋白间相互作用 作者:Magnez Romain, Bryan Thiroux, Morgane Tardy, Bruno Quesnel and Xavier Thuru日期:04/05/2020,浏览量:1499,Q&A: 0
The binding interactions of PD-1 and PD-L1 have been studied by surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC) over the past few years, but these investigations resulted in controversy regarding the values of the dissociation constant (Kd) (Freeman et al., 2000). MST is a powerful new method for the ...
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Spectrophotometric Assessment of Heme Oxygenase-1 Activity in Leishmania-infected Macrophages 利什曼原虫感染巨噬细胞中血红素加氧酶-1活性的分光光度法测定 作者:Moumita Basu, Shriya Saha and Anindita Ukil日期:04/05/2020,浏览量:1101,Q&A: 0
Heme oxygenase-1 (HO-1) is a stress responsive enzyme that metabolizes heme and releases free iron, carbon monoxide (CO), and biliverdin (BV), which rapidly undergoes conversion to bilirubin (BL). Estimation of bilirubin is the basis of HO-1 assay. HO-1 activity is widely employed to determine antioxidant response of cells under different ...
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Quantitative Determination of Ca2+-binding to Ca2+-sensor Proteins by Isothermal Titration Calorimetry 钙离子与钙离子传感器蛋白结合的等温滴定量热法定量测定 作者:Seher Abbas and Karl-Wilhelm Koch日期:04/05/2020,浏览量:1244,Q&A: 0
Diverse and complex molecular recognitions are central elements of signal transduction cascades. The strength and nature of these interaction modes can be determined by different experimental approaches. Among those, Isothermal titration calorimetry (ITC) offers certain advantages by providing binding constants and thermodynamic parameters from ...
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In vivo Tumor Growth and Spontaneous Metastasis Assays Using A549 Lung Cancer Cells 利用A549肺癌细胞进行肿瘤生长和自发性转移的体内检测 作者:Lei Qi, Teresa Knifley, Dava W. Piecoro, Piotr Rychahou, Jianrong Wu, Kathleen L. O’Connor and Min Chen日期:04/05/2020,浏览量:1504,Q&A: 0
Metastasis accounts for the majority of cancer related deaths. The genetically engineered mouse (GEM) models and cell line-based subcutaneous and orthotopic mouse xenografts have been developed to study the metastatic process. By using lung cancer cell line A549 as an example, we present a modified protocol to establish the cell line-based ...
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Flow Cytometry Measurement of Glucocerebrosidase Activity in Human Monocytes 人单核细胞中葡萄糖脑苷脂酶活性的流式细胞术检测 作者:Laura P. Hughes, Glenda M. Halliday and Nicolas Dzamko日期:04/05/2020,浏览量:1457,Q&A: 0
Glucocerebrosidase (GCase) is an important enzyme for the metabolism of glycolipids. GCase enzyme deficiency is implicated in human disease and the efficient measurement of GCase activity is important for evaluating the efficacy of therapeutics targeting this enzyme. Existing approaches to measure GCase activity include whole blood mass ...
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Isolation, Purification and Characterization of Exosomes from Fibroblast Cultures of Skeletal Muscle 骨骼肌成纤维细胞培养物中外泌体的分离、纯化、鉴定 作者:Diantha van de Vlekkert, Xiaohui Qiu, Ida Annunziata and Alessandra d'Azzo日期:04/05/2020,浏览量:1261,Q&A: 0
Exosomes are dynamic nanovesicles secreted by virtually all cells and are present in all biological fluids. Given their highly heterogeneous content exosomes have been implicated in many physiological and pathological processes that they exert by influencing cell-cell and cell-ECM communication. In recent years an increasing number of methods have ...
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Fractionation of Ovarian Follicles and in vitro Oocyte Maturation and Ovulation Assay of Ciona intestinalis Type A A型玻璃海鞘卵泡分级分离、体外卵母细胞成熟和排卵检测 作者:Shin Matsubara, Akira Shiraishi, Tomohiro Osugi, Tsuyoshi Kawada and Honoo Satake日期:04/05/2020,浏览量:1265,Q&A: 0
Ascidians are the closest living relatives of vertebrates (Delsuc et al., 2006; Satoh et al., 2014) and are important for the evolutionary study of the ovarian follicle development including oocyte maturation and ovulation. However, neither the endogenous factors nor the molecular mechanisms underlying the oocyte maturation and ...
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Bimolecular Fluorescence Complementation (BiFC) for Studying Sarcomeric Protein Interactions in Drosophila 双分子荧光互补法研究果蝇肌节蛋白相互作用 作者:Océane Marescal, Frieder Schöck and Nicanor González-Morales日期:04/05/2020,浏览量:1428,Q&A: 0
Protein-protein interactions in Drosophila myofibrils are essential for their function and formation. Bimolecular Fluorescence Complementation (BiFC) is an effective method for studying protein interactions and localization. BiFC relies on the reconstitution of a monomeric fluorescent protein from two half-fragments when in proximity. Two ...
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Conjugation Protocol Optimised for Roseburia inulinivorans and Eubacterium rectale 食葡糖罗斯拜瑞氏菌和直肠真杆菌结合的优化方案 作者:Paul O. Sheridan, Jennifer C. Martin and Karen P. Scott日期:04/05/2020,浏览量:1103,Q&A: 0
Roseburia and Eubacterium species of the human gut microbiota play an important role in the maintaince of human health, partly by producing butyrate, the main energy source of our colonic epithelial cells. However, our knowledge of the biochemistry and physiology of these bacteria has been limited by a lack of genetic ...
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Preparation of a Bacteriophage T4-based Prokaryotic-eukaryotic Hybrid Viral Vector for Delivery of Large Cargos of Genes and Proteins into Human Cells 用于将基因和蛋白质转运至人体细胞中的基于噬菌体T4的大容量原核真核杂交病毒载体的制备 作者:Jingen Zhu, Pan Tao, Marthandan Mahalingam and Venigalla B. Rao日期:04/05/2020,浏览量:921,Q&A: 0
A viral vector that can safely and efficiently deliver large and diverse molecular cargos into cells is the holy grail of curing many human diseases. Adeno-associated virus (AAV) has been extensively used but has a very small capacity. The prokaryotic virus T4 has a large capacity but lacks natural mechanisms to enter mammalian cells. Here, we ...
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Measuring cAMP Specific Phosphodiesterase Activity: A Two-step Radioassay cAMP磷酸二酯酶特异活性测定:一种两步放射分析法 作者:Connor M. Blair, Jiayue Ling and George S. Baillie日期:04/05/2020,浏览量:853,Q&A: 0
Cyclic nucleotide degrading phosphodiesterase (PDE) enzymes are crucial to the fine tuning of cAMP signaling responses, playing a pivotal role in regulating the temporal and spatial characteristics of discrete cAMP nanodomains and hence the activity of cAMP-effector proteins. As a consequence of orchestrating cAMP homeostasis, dysfunctional PDE ...
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Real-time Fluorescence Measurement of Enterovirus Uncoating 肠道病毒脱壳的实时荧光检测 作者:Visa Ruokolainen, Mira Laajala and Varpu Marjomäki日期:04/05/2020,浏览量:1007,Q&A: 0
Viruses need to open, i.e., uncoat, in order to release their genomes for efficient replication and translation. Especially for non-enveloped viruses, such as enteroviruses, the cues leading to uncoating are less well known. The status of the virus has previously been observed mainly by transmission electron microscopy using negative ...
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A Simple and Fast Battery Test for Phenotypic Characterization of Mice 小鼠表型特征的简易快速系列测试试验 作者:Mariana S. A. Garcia-Gomes, Dennis A. Zanatto, Pedro K. Yamamoto, Danilo Wadt, Ana T. F. B. Antiorio, Jilma Aleman-Laporte, Sandra R. Alexandre-Ribeiro, Guilherme A. Marson, Cezar Guizzo, Silvia M. G. Massironi, Maria M. Bernardi and Claudia M. C. Mori日期:04/05/2020,浏览量:1070,Q&A: 0
Despite the great number of test batteries already known to assess the behavior of genetically modified and inbred strains of mice, only a few of them focus on basic neurological parameters. The purpose of the battery test proposed is to settle a specific methodology to characterize the phenotype of neurological disease models in mutant or ...
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