• 卷册 10, 2020
  • 卷册 9, 2019
  • 卷册 8, 2018
  • 卷册 7, 2017
  • 卷册 6, 2016
  • 卷册 5, 2015
  • 卷册 4, 2014
  • 卷册 3, 2013
  • 卷册 2, 2012
  • 卷册 1, 2011
Image Credit: BioProtoc.3594

往期刊物(2020)

卷册: 10 期号: 8

生物化学

Fibrin Breakdown Assay 纤维蛋白分解检测 作者:Jiayue Ling, Connor M. Blair and George S. Baillie日期:04/20/2020,浏览量:1521,Q&A: 0
Fibrinolysis is an integral part of the matrix remodeling process that contributes to tissue repair. Fibrin clots are broken down during fibrinolysis in a controlled process. Fibrin degradation products (FDPs) have also been shown to have a role in the regulation of cell growth and are implicated in various vascular diseases. This protocol was ...
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Enzymatic Construction of Protein Polymer/Polyprotein Using OaAEP1 and TEV Protease 利用OaAEP1和TEV蛋白酶进行蛋白聚合物/多聚蛋白的酶促构建 作者:Yibing Deng, Shengchao Shi, Bin Zheng, Tao Wu and Peng Zheng日期:04/20/2020,浏览量:1569,Q&A: 0
The development of chemical and biological coupling technologies in recent years has made possible of protein polymers engineering. We have developed an enzymatic method for building polyproteins using a protein ligase OaAEP1 (asparagine endopeptidase 1) and protease TEV (tobacco etching virus). Using a mobile TEV protease site compatible with the ...
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癌症生物学

Single Cell Migration Assay Using Human Breast Cancer MDA-MB-231 Cell Line 利用人乳腺癌MDA-MB-231细胞系进行单细胞迁移分析 作者:David M. Gau and Partha Roy日期:04/20/2020,浏览量:1969,Q&A: 0
Cell migration is a fundamental cellular process that plays a crucial role in many physioglogical and pathological processes such as wound healing or cancer metastasis. Many assays have been developed to examine cell migration, such as the wound healing or scratch assay, Boyden Chamber or transwell assay, and the method we will describe here, ...
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细胞生物学

Dual Fluorescence Cytometry Assay to Assess Cellular Protein Levels 双荧光细胞仪检测细胞蛋白水平 作者:Ashley M Ngo, Ruofan Wang and Andreas S Puschnik日期:04/20/2020,浏览量:1514,Q&A: 0
Expression levels of cellular proteins can be affected by various perturbations, such as genetic knockout of interactors, drug treatments or cell stress. To specifically measure the effects on protein levels post-synthesis under different experimental conditions, it is important to compensate for transcriptional and other upstream changes. Here, ...
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发育生物学

Auxin-mediated Protein Degradation in Caenorhabditis elegans 生长素介导的秀丽隐杆线虫蛋白质降解 作者:Michael A. Q. Martinez and David Q. Matus日期:04/20/2020,浏览量:1700,Q&A: 0
The auxin-inducible degron (AID) technology was recently adapted for use in the nematode Caenorhabditis elegans. Rapid degradation of C. elegans proteins tagged with an AID is mediated by a plant-specific F-box protein, transport inhibitor response 1 (TIR1), and occurs only in the presence of the phytohormone auxin. The first ...
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免疫学

A Triple-challenge Mouse Model of Allergic Airway Disease, Primary Influenza Infection, and Secondary Bacterial Infection 气道变态反应性疾病,原发性流感感染,继发性细菌感染的三重小鼠模型 作者:Sean Roberts, Clare M Williams, Sreeja Roy and Yoichi Furuya日期:04/20/2020,浏览量:968,Q&A: 0
Asthma is a global problem that affects millions of individuals. An increased risk of respiratory viral and bacterial infections is one of the complications of asthma. We recently reported that mice with ovalbumin-induced allergic airway disease (AAD) are protected against influenza-Streptococcus pneumoniae co-infection. Here, we describe ...
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Environmental Conditioning and Aerosol Infection of Mice 环境调节与小鼠气溶胶感染 作者:Eriko Kudo and Akiko Iwasaki日期:04/20/2020,浏览量:1275,Q&A: 0
Influenza infection models in mice are widely used to study flu-mediated immune responses and pathology. However, most laboratory mice are housed at 20 °C and 50% relative humidity (RH). To better recapitulate influenza epidemics and immune responses during winter seasons, mice were housed at 20 °C under different humidity conditions, 10-20% or ...
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微生物学

In vivo Quantification of Alkanes in Escherichia coli 大肠杆菌中脂肪醛去甲酰加氧酶产生烷烃的体内量化 作者:Tabinda Shakeel, Zia Fatma and Syed Shams Yazdani日期:04/20/2020,浏览量:1353,Q&A: 0
Microbial production of alkanes employing synthetic biology tools has gained tremendous attention owing to the high energy density and similarity of alkanes to existing petroleum fuels. One of the most commonly studied pathways includes the production of alkanes by AAR (acyl-ACP (acyl carrier protein) reductase)-ADO (aldehyde deformylating ...
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分子生物学

Isolation and Characterization of Exosomes from Mouse Feces 小鼠粪便中外泌体的分离和鉴定 作者:Chunhua Yang, Mingzhen Zhang, Junsik Sung, Lixin Wang, Yunjin Jung and Didier Merlin日期:04/20/2020,浏览量:1598,Q&A: 0
Exosomes secreted by colonic epithelial cells are present in feces and contain valuable epigenetic information, such as miRNAs, proteins, and metabolites. An in-depth study of this information is conducive to the diagnosis or treatment of relevant diseases. A crucial prerequisite of such a study is to establish an efficient isolation method, ...
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An in vitro Assay to Screen for Substrates of PKA Using Phospho-motif-specific Antibodies 利用磷酸基团特异性抗体体外筛选PKA底物 作者:David M. Gau and Partha Roy日期:04/20/2020,浏览量:1063,Q&A: 0
Kinases function as regulators of many cellular processes such as cell migration. These enzymes typically phosphorylate target motif sequences. Mass spec or phospho-specific antibody detection can be used to determine whether a kinase can phosphorylate proteins of interest, however, mass spec can be expensive and phospho-antibodies for the protein ...
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High-throughput Flow Cytometry Assay to Investigate TDP43 Splicing Function 高通量流式细胞术检测TDP43剪接功能 作者:H. Broder Schmidt and Rajat Rohatgi日期:04/20/2020,浏览量:1488,Q&A: 0
Mutations in RNA-binding proteins (RBPs) such as TDP43 are associated with transcriptome-wide splicing defects and cause severe neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The impact of RBP mutations on splicing function is routinely studied using PCR-based bulk measurements. ...
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神经科学

Minimally Invasive Oral Surgery Induction of the FRICT-ION Chronic Neuropathic Pain Model 微创口腔手术诱导FRICT-ION慢性神经病理性疼痛模型的建立 作者:Marena A. Montera and Karin N. Westlund日期:04/20/2020,浏览量:1049,Q&A: 0
An easily induced preclinical trigeminal neuropathic nerve injury model is described here for the study of chronic pain, the model acronym FRICT-ION (Foramen Rotundum Inflammatory Constriction Trigeminal InfraOrbital Nerve). In patients, neuropathic pain is thought to be related to ...
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Examining Cocaine Conditioning Place Preference in Mice 可卡因调节小鼠位置偏爱研究 作者:MaryElizabeth J. Simkevich, Rianne R. Campbell and André O. White日期:04/20/2020,浏览量:1020,Q&A: 0
A key component of combating substance use disorders is understanding the neural mechanisms that support drug reward. Tasks such as self-administration assess the reinforcing properties of a drug using a learned behavior but require numerous training sessions and surgery. In comparison, the conditioned place preference (CPP) task assesses reward ...
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植物科学

MAMP-triggered Medium Alkalinization of Plant Cell Cultures MAMP诱导植物细胞培养基质碱化 作者:Gabriel L. Fiorin, Andrea Sánchez-Vallet, Bart P. H. J. Thomma, Gonçalo A. G. Pereira and Paulo J.P.L. Teixeira日期:04/20/2020,浏览量:1258,Q&A: 0
Plants recognize a wide variety of microbial molecules to detect and respond to potential invaders. Recognition of Microbe-Associated Molecular Patterns (MAMPs) by cell surface receptors initiate a cascade of biochemical responses that include, among others, ion fluxes across the plasma membrane. A consequence of such event is a decrease in the ...
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系统生物学

Circadian Gene Profiling in Laser Capture Microdissected Mouse Club Cells 激光捕获显微解剖小鼠棒状细胞的昼夜节律基因分析 作者:Zhenguang Zhang and Andrew Loudon日期:04/20/2020,浏览量:1100,Q&A: 0
Cell heterogeneity is high in tissues like lung. Research conducted on pure population of cells usually offers more insights than bulk tissues, such as circadian clock work. In this protocol, we provide a detailed work flow on how to do circadian clock study by RNA seq in laser capture micro-dissected mouse lung club cells. The method uses frozen ...
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