• 卷册 10, 2020
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  • 卷册 8, 2018
  • 卷册 7, 2017
  • 卷册 6, 2016
  • 卷册 5, 2015
  • 卷册 4, 2014
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  • 卷册 2, 2012
  • 卷册 1, 2011
Image Credit: BioProtoc.3603


卷册: 10 期号: 9


Confocal and Super-resolution Imaging of RNA in Live Bacteria Using a Fluorogenic Silicon Rhodamine-binding Aptamer 含氟硅-罗丹明适配体用于活性菌中RNA的激光共聚焦超分辨率成像 作者:Regina Wirth, Peng Gao, G. Ulrich Nienhaus, Murat Sunbul and Andres Jäschke日期:05/05/2020,浏览量:1761,Q&A: 0
Genetically encoded light-up RNA aptamers have been shown to be promising tools for the visualization of RNAs in living cells, helping us to advance our understanding of the broad and complex life of RNA. Although a handful of light-up aptamers spanning the visible wavelength region have been developed, none of them have yet been reported to be ...
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Quantification of Fatty Acids in Mammalian Tissues by Gas Chromatography–Hydrogen Flame Ionization Detection 气相色谱-氢火焰离子化检测法测定哺乳动物组织中的脂肪酸 作者:Fumie Hamano, Suzumi M Tokuoka, Tomomi Hashidate-Yoshida, Hideo Shindou, Takao Shimizu and Yoshihiro Kita日期:05/05/2020,浏览量:1316,Q&A: 0
In mammalian organisms, fatty acids (FAs) exist mostly in esterified forms, as building blocks of phospholipids, triglycerides, and cholesteryl esters, while some exist as non-esterified free FAs. The absolute quantification of FA species in total lipids or in a specific lipid class is critical in lipid-metabolism studies. To quantify FAs in ...
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Colorimetric RhoB GTPase Activity Assay 比色法测定RhoB-GTPase活性 作者:Kossay Zaoui and Stephanie Duhamel日期:05/05/2020,浏览量:1583,Q&A: 0
The Ras homologous protein (Rho) GTPase subfamily, including RhoA, RhoB, and RhoC are small molecules (~21 kDa) that act as molecular switches in a wide range of signaling pathways to orchestrate biological processes associated with both physiological and tumorigenic cellular states. The Rho GTPases are crucial regulators of actin cytoskeleton ...
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A Modified Semisolid Clonal Culture for Identification of B-1 and B-2 Progenitor Colony Forming Ability of Mouse Embryonic Hemogenic Endothelial Cells 改良半固态克隆培养鉴定小鼠胚胎造血内皮细胞B-1和B-2祖细胞集落形成能力 作者:Michihiro Kobayashi and Momoko Yoshimoto日期:05/05/2020,浏览量:1025,Q&A: 0
The search for the origin of the first hematopoietic stem cells (HSCs) in the mouse embryo has been a hot topic in the field of developmental hematopoiesis. Detecting lymphoid potential is one of the supportive evidence to show the definitive hematopoietic activity of HSCs. However, the first B-lymphoid potential in the mouse embryos are reported ...
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Application of Mechanical Forces on Drosophila Embryos by Manipulation of Microinjected Magnetic Particles 机械力应用于果蝇胚胎的磁性粒子显微注射操作 作者:Arturo D’Angelo and Jérôme Solon日期:05/05/2020,浏览量:983,Q&A: 0
Cells generate mechanical forces to shape tissues during morphogenesis. These forces can activate several biochemical pathways and trigger diverse cellular responses by mechano-sensation, such as differentiation, division, migration and apoptosis. Assessing the mechano-responses of cells in living organisms requires tools to apply controlled local ...
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Live Cell Imaging of Male Meiosis in Arabidopsis by a Landmark-based System 地标系统在拟南芥雄性减数分裂活体细胞成像中的应用 作者:Maria Ada Prusicki, Emma Mathilde Keizer, Rik Peter van Rosmalen, Christian Fleck and Arp Schnittger日期:05/05/2020,浏览量:1512,Q&A: 0
Live cell imaging has tremendously promoted our understanding of cellular and subcellular processes such as cell division. Here, we present a step-by-step protocol for a robust and easy-to-use live cell imaging approach to study male meiosis in the plant Arabidopsis thaliana as recently established. Our method relies on the concomitant ...
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Evaluation of B Cell Proliferation in vivo by EdU Incorporation Assay 通过EdU掺入法评估体内B细胞增殖 作者:Adi Biram and Ziv Shulman日期:05/05/2020,浏览量:1259,Q&A: 0
Generation of antibodies is crucial for establishing enduring protection from invading pathogens, as well as for maintaining homeostasis with commensal bacteria at mucosal surfaces. Chronic exposure to microbiota- and dietary- derived antigens results in continuous production of antibody producing cells within the Peyer’s patch germinal center ...
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Assessing in vitro and in vivo Trogocytosis By Murine CD4+ T cells 用小鼠CD4+T细胞评价体内外胞啃作用 作者:Jim Reed and Scott A. Wetzel日期:05/05/2020,浏览量:1139,Q&A: 0
Recognition of antigens by lymphocytes (B, T, and NK) on the surface of an antigen-presenting cell (APC) leads to lymphocyte activation and the formation of an immunological synapse between the lymphocyte and the APC. At the immunological synapse APC membrane and associated membrane proteins can be transferred to the lymphocyte in a process called ...
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HIV-CRISPR: A CRISPR/Cas9 Screening Method to Identify Genes Affecting HIV Replication HIV-CRISPR:一种CRISPR/Cas9筛选HIV复制相关基因的方法 作者:Ferdinand Roesch and Molly OhAinle日期:05/05/2020,浏览量:1230,Q&A: 0
Screening with CRISPR/Cas9 technology has already led to significant discoveries in the fields of cancer biology, cell biology and virology. Because of the relatively low false discovery rates and the ability to perform high-throughput, pooled approaches, it has rapidly become the assay of choice for screening studies, including whole-genome ...
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Viral Double-Stranded RNA Detection by DNase I and Nuclease S1 digestions in Leishmania parasites 在利什曼原虫中利用DNase I和 Nuclease S1酶解进行病毒双链RNA鉴定 作者:Nathalie Isorce and Nicolas Fasel日期:05/05/2020,浏览量:830,Q&A: 0
Many RNA viruses are found in protozoan parasites. They can be responsible for more serious pathology or treatment failure. For the detection of viral double-stranded RNA (dsRNA), sequence-dependent and -independent methods are available, such as quantitative real-time PCR and immunofluorescence, dot blot, ELISA or sequencing. The technique ...
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A Sensitive Coupled Enzyme Assay for Measuring Kinase and ATPase Kinetics Using ADP-Specific Hexokinase 使用ADP特异性己糖激酶测量激酶和ATPase动力学的灵敏耦合酶活测定 作者:Ciaran R. McFarlane and James W. Murray日期:05/05/2020,浏览量:1192,Q&A: 0
Kinases and ATPases perform essential biological functions in metabolism and regulation. Activity of these enzymes is commonly measured by coupling ATP consumption to the synthesis of a detectable product. For most assay systems the ATP concentration during the reaction is unknown, compromising the precision of the assay.

Using the ...
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Quantification of Bacteria Residing in Caenorhabditis elegans Intestine 秀丽隐杆线虫肠道细菌数量测定 作者:M. Fernanda Palominos and Andrea Calixto日期:05/05/2020,浏览量:1250,Q&A: 0
Quantification of intestinal colonization by pathogenic or commensal bacteria constitute a critical part of the analysis to understand host-microbe interactions during different time points of their interplay. Here we detail a method to isolate non-pathogenic and pathogenic bacteria from C. elegans intestines, and classify gut phenotypes ...
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Split Nano Luciferase-based Assay to Measure Assembly of Japanese Encephalitis Virus 基于分割纳米荧光素酶的乙型脑炎病毒组装检测 作者:Simon Goto, Kotaro Ishida, Ryosuke Suzuki and Eiji Morita日期:05/05/2020,浏览量:762,Q&A: 0
Cells infected with flavivirus release various forms of infectious and non-infectious particles as products and by-products. Comprehensive profiling of the released particles by density gradient centrifugation is informative for understanding viral particle assembly. However, it is difficult to detect low-abundance minor particles in such ...
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Rapid Generation of Human Neuronal Cell Models Enabling Inducible Expression of Proteins-of-interest for Functional Studies 快速建立能诱导表达功能研究所需蛋白的人神经细胞模型 作者:Xinzhu Wang, Erik Friesen, Iris Müller, Mackenzie Lemieux, Ramona Dukart, Isabella BL Maia, Suneil Kalia and Gerold Schmitt-Ulms日期:05/05/2020,浏览量:1170,Q&A: 0
CRISPR-Cas9 technology has transformed the ability to edit genomic sequences and control gene expression with unprecedented ease and scale. However, precise genomic insertions of coding sequences using this technology remain time-consuming and inefficient because they require introducing adjacent single-strand cuts through Cas9 nickase action and ...
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Sequence Alignment Using Machine Learning for Accurate Template-based Protein Structure Prediction 基于模板的蛋白质结构精确预测的机器学习序列比对 作者:Shuichiro Makigaki and Takashi Ishida日期:05/05/2020,浏览量:1311,Q&A: 0
Template-based modeling, the process of predicting the tertiary structure of a protein by using homologous protein structures, is useful when good templates can be available. Indeed, modern homology detection methods can find remote homologs with high sensitivity. However, the accuracy of template-based models generated from the ...
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