• 卷册 10, 2020
  • 卷册 9, 2019
  • 卷册 8, 2018
  • 卷册 7, 2017
  • 卷册 6, 2016
  • 卷册 5, 2015
  • 卷册 4, 2014
  • 卷册 3, 2013
  • 卷册 2, 2012
  • 卷册 1, 2011
Image Credit: BioProtoc.3628

往期刊物(2020)

卷册: 10 期号: 10

生物化学

Permethylation and Microfractionation of Sulfated Glycans for MS Analysis 硫酸多糖的全甲基化和微萃取质谱分析 作者:Shin-Yi Yu, Sergei Snovida and Kay-Hooi Khoo日期:05/20/2020,浏览量:1191,Q&A: 0
Sulfated glycans are barely detectable in routine mass spectrometry (MS)-based glycomic analysis due to ion suppression by the significantly more abundant neutral glycans in the positive ion mode, and sialylated non-sulfated glycans in the negative ion mode, respectively. Nevertheless, the negative charge imparted by sulfate can be advantageous ...
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Negative Ion Mode nanoLC-ESI-MS/MS Analyses of Permethylated Sulfated Glycans 甲基化硫酸多糖的负离子模式nanoLC-ESI-MS / MS分析 作者:Shin-Yi Yu, Chu-Wen Cheng and Kay-Hooi Khoo日期:05/20/2020,浏览量:1110,Q&A: 0
We have developed enabling techniques for sulfoglycomics based on MALDI-MS mapping and MS/MS sequencing of permethylated sulfated glycans. We then extended further the analytical workflow to C18 reverse phase (RP)-nanoLC-nanoESI-MS/MS analyses of permethylated sulfated glycans in the negative ion mode. The advantages are that extra sulfates on ...
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Evaluation of the Efficiency of Genome Editing Tools by a Frameshift Fluorescence Protein Reporter 通过移码荧光蛋白报告基因评估基因组编辑工具的效率 作者:Balaji T. Moorthy, Akhilesh Kumar, Lauren X. Lotenfoe and Fangliang Zhang日期:05/20/2020,浏览量:1380,Q&A: 0
In the last decade, genome editing has been the center of attention as a novel tool for mechanistic investigations and for potential clinical applications. Various genome editing tools like meganucleases, zinc finger nucleases (ZFNs), transcription activator-like effector-based nucleases (TALEN), and the clustered regularly interspaced short ...
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In vitro Assay for Bacterial Membrane Protein Integration into Proteoliposomes 细菌膜蛋白在蛋白质脂质体中的体外整合 作者:Hanako Nishikawa, Masaru Sasaki and Ken-ichi Nishiyama日期:05/20/2020,浏览量:1364,Q&A: 0
It is important to experimentally determine how membrane proteins are integrated into biomembranes to unveil the roles of the integration factors, and to understand the functions and structures of membrane proteins. We have developed a reconstitution system for membrane protein integration in E. coli using purified factors, in which the ...
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癌症生物学

Modeling NOTCH1 driven T-cell Acute Lymphoblastic Leukemia in Mice NOTCH1诱导小鼠T细胞急性淋巴细胞白血病模型的建立 作者:Agnieszka A. Wendorff and Adolfo A. Ferrando日期:05/20/2020,浏览量:1411,Q&A: 0
T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematological malignancy that arises from transformation of T-cell primed hematopoietic progenitors. Although T-ALL is a heterogenous and molecularly complex disease, more than 65% of T-ALL patients carry activating mutations in the NOTCH1 gene. The majority of T-ALL–associated ...
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Isolation of Tumor Cells Based on Their Distance from Blood Vessels 基于与血管距离的肿瘤细胞分离 作者:Saran Kumar, Husni Sharife, Tirzah Kreisel, Libat Bar-Lev, Myriam Grunewald and Eli Keshet日期:05/20/2020,浏览量:1490,Q&A: 0
Differential exposure of tumor cells to microenvironmental cues greatly impacts cell phenotypes, raising a need for position based sorting of tumor cells amenable to multiple OMICs and functional analyses. One such key determinant of tumor heterogeneity in solid tumors is its vasculature. Proximity to blood vessels (BVs) profoundly affects tumor ...
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细胞生物学

Spatial Image Correlation Spectroscopy (ICS): A Technique for Average Size Determination of Subcellular Accumulated Structures from Fluorescence Microscopic Images 空间图像相关光谱法(ICS):一种从荧光显微图像中确定亚细胞聚集结构平均尺寸的技术 作者:Akira Kitamura and Masakata Kinjo日期:05/20/2020,浏览量:1173,Q&A: 0
Size determination of subcellular structures such as inclusion bodies (IBs) and granules from fluorescent images is important for identification and structural characterization. However, it is often time-consuming just for the comparison of the average size of the structures. Here, we introduce a high-throughput procedure to represent the average ...
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Pancreatic Acinar Cell Preparation for Oxygen Consumption and Lactate Production Analysis 用于耗氧量和乳酸生产分析的胰腺腺泡细胞制备 作者:Jane A. Armstrong, Robert Sutton and David N. Criddle日期:05/20/2020,浏览量:1022,Q&A: 0
Mitochondrial dysfunction is a principal feature of acute pancreatitis (AP) although the underlying mechanisms are still unclear. AP precipitants induce Ca2+-dependent formation of the mitochondrial permeability transition pore (MPTP) in pancreatic acinar cells (PACs), leading to ATP depletion and necrosis. Evaluations of mitochondrial ...
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Analysis of Generalized Fibrosis in Mouse Tissue Sections with Masson’s Trichrome Staining Masson Trichrome染色法分析小鼠组织切片中的普遍纤维化 作者:Diantha van de Vlekkert, Eda Machado and Alessandra d’Azzo日期:05/20/2020,浏览量:1202,Q&A: 0
Expansion of fibrous connective tissue and abnormal deposition of extracellular matrix (ECM) are at the basis of many fibrotic diseases. Fibrosis can occur in response to both physiological and pathological cues, including wound healing, tissue remodeling/repair and inflammation. Chronic fibrosis can lead to severe tissue damage, organ failure and ...
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发育生物学

Direct Reprogramming of Mouse Embryonic Fibroblasts to Conventional Type 1 Dendritic Cells by Enforced Expression of Transcription Factors 转录因子强化表达直接将小鼠胚胎成纤维细胞重编程为常规1型树突状细胞 作者:Fábio F. Rosa, Cristiana F. Pires, Olga Zimmermannova and Carlos-Filipe Pereira日期:05/20/2020,浏览量:1437,Q&A: 0
Ectopic expression of transcription factor combinations has been recently demonstrated to reprogram differentiated somatic cells towards the dendritic cell (DC) lineage without reversion to a multipotent state. DCs have the ability to induce potent and long-lasting adaptive immune responses. In particular, conventional type 1 DCs (cDC1s) excel on ...
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免疫学

Flow Cytometry Analysis and Fluorescence-activated Cell Sorting of Myeloid Cells from Lung and Bronchoalveolar Lavage Samples from Mycobacterium tuberculosis-infected Mice 结核分枝杆菌感染小鼠肺和支气管肺泡灌洗样品中髓样细胞的流式细胞仪分析和荧光激活细胞分选 作者:Alissa C. Rothchild, Dat Mai, Alan Aderem and Alan H. Diercks日期:05/20/2020,浏览量:1599,Q&A: 0
Mycobacterium tuberculosis (Mtb) is transmitted by aerosol and can cause serious bacterial infection in the lung that can be fatal if left untreated. Mtb is now the leading cause of death worldwide by an infectious agent. Characterizing the early events of in vivo infection following aerosol challenge is critical for ...
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微生物学

Bacterial Lawn Avoidance and Bacterial Two Choice Preference Assays in Caenorhabditis elegans 秀丽隐杆线虫的细菌草坪趋避和细菌双选择参照实验 作者:Jogender Singh and Alejandro Aballay日期:05/20/2020,浏览量:1224,Q&A: 0
Physical avoidance of pathogens is a crucial defense strategy used by the host to reduce pathogen infection. Hosts display the use of multiple strategies to sense and avoid pathogens, ranging from olfaction to sensing of damage caused by pathogen infection. Understanding various mechanisms of pathogen avoidance has the potential to uncover ...
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分子生物学

Molecular Size Analysis of Recombinant Importin-histone Complexes Using Analytical Ultracentrifugation 重组输入蛋白-组蛋白配合物分子大小的超离心分析 作者:Abhilash Padavannil, Chad A. Brautigam and Yuh Min Chook日期:05/20/2020,浏览量:1093,Q&A: 0
Histones constitute the protein components of nucleosomes. Despite their small sizes, histones do not diffuse through the nuclear pore complex. Instead, they are transported to the nucleus by importins, either alone or in complex with histone chaperones. Determining the molecular size of the importin-histone complexes is key to understanding the ...
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神经科学

Generation, Analyzing and in-vivo Drug Treatment of Drosophila Models with IBMPFD 果蝇IBMPFD模型的建立、分析及体内药物治疗 作者:Ting Zhang, Bruce A. Hay and Ming Guo日期:05/20/2020,浏览量:1066,Q&A: 0
Missense mutations of p97/cdc48/Valosin-containing protein (VCP) cause inclusion body myopathy, Paget disease with frontotemporal dementia (IBMPFD) and other neurodegenerative diseases. The pathological mechanism of IBMPFD is not clear and there is no treatment. We generated Drosophila models of IBMPFD in adult flight muscle in ...
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植物科学

Purification of Protein-complexes from the Cyanobacterium Synechocystis sp. PCC 6803 Using FLAG-affinity Chromatography Cyanobacterium Synechocystis sp. PCC 6803 中蛋白混合物的标记亲和层析纯化方法 作者:Minna M. Koskela, Petra Skotnicová, Éva Kiss and Roman Sobotka日期:05/20/2020,浏览量:1261,Q&A: 0
Exploring the structure and function of protein complexes requires their isolation in the native state–a task that is made challenging when studying labile and/or low abundant complexes. The difficulties in preparing membrane-protein complexes are especially notorious. The cyanobacterium Synechocystis sp. PCC 6803 is a widely used model ...
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