• 卷册 10, 2020
  • 卷册 9, 2019
  • 卷册 8, 2018
  • 卷册 7, 2017
  • 卷册 6, 2016
  • 卷册 5, 2015
  • 卷册 4, 2014
  • 卷册 3, 2013
  • 卷册 2, 2012
  • 卷册 1, 2011
Image Credit: BioProtoc.3643

往期刊物(2020)

卷册: 10 期号: 11

生物化学

Isolating Multiple Extracellular Vesicles Subsets, Including Exosomes and Membrane Vesicles, from Bovine Milk Using Sodium Citrate and Differential Ultracentrifugation 用柠檬酸钠和差速超速离心分离牛乳中多个含外泌体和膜囊泡的胞外小泡亚群 作者:Abderrahim Benmoussa, Sara Michel, Caroline Gilbert and Patrick Provost日期:06/05/2020,浏览量:1365,Q&A: 0
Milk is a complex fluid that contains various types of proteins and extracellular vesicles (EVs). Some proteins can mingle with EVs, and interfere with their isolation. Among these proteins, caseins form micelles of a size comparable to milk EVs, and can thus be co-isolated with EVs. Preliminary steps that affect milk are crucial for EV isolation ...
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Biochemical Pulldown of mRNAs and Long Noncoding RNAs from Cellular Lysates Coupled with Mass Spectrometry to Identify Protein Binding Partners 通过细胞裂解液中mRNAs和长链非编码RNA的生化沉降结合质谱法鉴定蛋白结合物 作者:Anca F. Savulescu, Stoyan Stoychev, Sipho Mamputha and Musa M. Mhlanga日期:06/05/2020,浏览量:1315,Q&A: 0
RNA binding proteins (RBPs) interact with cellular mRNAs, controlling various steps throughout the lifetime of these transcripts, including transcription, cellular transport, subcellular localization, translation and degradation. In addition to binding mRNA transcripts, a growing number of RBPs are shown to bind long noncoding RNAs (lncRNAs), ...
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细胞生物学

Extracellular Vesicles Tracking and Quantification Using CT and Optical Imaging in Rats 大鼠细胞外囊泡的CT和光学成像追踪与定量研究 作者:Shaowei Guo, Oshra Betzer, Nisim Perets, Shira Landau, Daniel Offen, Rachela Popovtzer and Shulamit Levenberg日期:06/05/2020,浏览量:1292,Q&A: 0
Exosomes, a subtype of extracellular vesicles, are nanovesicles of endocytic origin. Exosomes contain a plethora of proteins, lipids, and genetic materials of parent cells to facilitate intercellular communications. Tracking exosomes in vivo is fundamentally important to understand their biodistribution pattern and the mechanism of ...
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Ex vivo Culture Assay Using Human Hair Follicles to Study Circadian Characteristics 通过人类毛囊体外培养研究昼夜节律特征 作者:Atsuhiro Nishida, Yoshiki Miyawaki, Koichi Node and Makoto Akashi日期:06/05/2020,浏览量:874,Q&A: 0
Ex vivo culture assays of biopsy specimens are advantageous for the experimental evaluation of human circadian characteristics. We developed a simple and non-invasive experimental evaluation method for monitoring the expression of circadian clock genes in an ex vivo culture assay using human hair follicles. This method imposes ...
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发育生物学

FRET Reporter Assays for cAMP and Calcium in a 96-well Format Using Genetically Encoded Biosensors Expressed in Living Cells 利用活细胞表达的基因编码生物传感器对96孔甲酸钙和cAMP的FRET检测 作者:Brandon T. Milliken, Robert P. Doyle, George G. Holz and Oleg G. Chepurny日期:06/05/2020,浏览量:1418,Q&A: 0
Stimulation of G protein-coupled receptors (GPCR) by hormones and neurotransmitters elicits cellular responses, many of which result from alterations in the concentrations of cytosolic cAMP and Ca2+. Here, we describe a microplate reader fluorescence resonance energy transfer (FRET) assay that uses the genetically encoded biosensors ...
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Primed Track: Reliable Volumetric Single-cell Tracking and Lineage Tracing of Living Specimen with Dual-labeling Approaches 引物轨迹:可靠的单细胞体积追踪和双标记活标本的谱系追踪 作者:Maaike Welling, Konstantinos Kalyviotis and Periklis Pantazis日期:06/05/2020,浏览量:1030,Q&A: 0
Mammalian embryonic development starts with a single fertilized zygote that develops into a blastocyst embryo consisting of three cell types that evolve into either embryonic or extra-embryonic tissues. Lineage tracing of these cells can provide important information about the molecular and cellular dynamics contributing to fate allocation during ...
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免疫学

RNA Extraction from Ears and Draining Lymph Nodes of Mice Infected with Leishmania amazonensis 亚马逊利什曼原虫感染小鼠耳及引流淋巴结RNA的提取 作者:Emilie Giraud and Evie Melanitou日期:06/05/2020,浏览量:987,Q&A: 0
Parasites of the genus Leishmania infect the mammalian hosts, including mice and humans and cause cutaneous or visceral leishmaniasis depending upon the parasite species transmitted by the vector sandfly. Leishmania amazonensis is one of the Leishmania species responsible for the cutaneous form of the disease. We have ...
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Isolation and High Throughput Flow Cytometric Apoptosis Assay of Human Neutrophils to Enable Compound Library Screening 人中性粒细胞的分离及高通量流式细胞术凋亡分析用于复合文库筛选 作者:K. D. Herman, A. Rahman and L. R. Prince日期:06/05/2020,浏览量:1027,Q&A: 0
The study of human neutrophils in vitro is challenging due to their short half-life and propensity for activation. However, with careful handling and manipulation in the laboratory, they can be a powerful tool to investigate immune responses in health and disease. Here we describe a method for the isolation of human neutrophils from ...
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Methodology for in vitro Assessment of Human T Cell Activation and Blockade 人体T细胞活化与阻断的体外评价方法 作者:Joseph R. Podojil, Igal Ifergan, Ming-Yi Chiang, Joshua J. Meeks and Stephen D. Miller日期:06/05/2020,浏览量:1099,Q&A: 0
Methods to test both the functionality and mechanism of action for human recombinant proteins and antibodies in vitro have been limited by multiple factors. To test the functionality of a recombinant protein or antibody, the receptor, the receptor-associated ligand, or both must be expressed by the cells present within the in vitro ...
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微生物学

Differential Fractionation of Erythrocytes Infected by Plasmodium berghei 感染伯氏疟原虫红细胞的差异分离 作者:Bénédicte Gnangnon, Véronique Peucelle and Christine Pierrot日期:06/05/2020,浏览量:743,Q&A: 0
The study of host/pathogen interactions at the cellular level during Plasmodium intra-erythrocytic cycle requires differential extraction techniques aiming to analyze the different compartments of the infected cell. Various protocols have been proposed in the literature to study specific compartments and/or membranes in the infected ...
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分子生物学

Mouse Adipose Tissue Protein Extraction 小鼠脂肪组织蛋白提取 作者:Yu A. An and Philipp E. Scherer日期:06/05/2020,浏览量:1473,Q&A: 0
As obesity becomes a global epidemic, the metabolism research field is increasingly focusing on studying the physiological and pathological roles of adipose tissues (AT). However, extracting proteins from AT is challenging due to abundant fat content of intracellular lipid droplets. Several commercial kits for extraction of AT proteins are ...
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神经科学

Enriched Environment Procedures for Rodents: Creating a Standardized Protocol for Diverse Enrichment to Improve Consistency across Research Studies 啮齿动物的环境丰容程序:创建提高研究一致性的多样化富集标准化协议 作者:Bruce C. Harland and John C. Dalrymple-Alford日期:06/05/2020,浏览量:684,Q&A: 0
Exposure to environmental enrichment has beneficial effects on learning and memory, diverse neurobiological effects, and promotes recovery of function after brain injury. The effect of enrichment is produced by a combination of increased social interaction, physical activity, spatial complexity, and novelty. Procedures in the literature have, ...
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Precise Targeting of Single Microelectrodes to Orientation Pinwheel Centers 单微电极精确定位风车中心区 作者:Xue Mei Song, Ming Li, Tao Xu, Dewen Hu and Anna Wang Roe日期:06/05/2020,浏览量:662,Q&A: 0
In the mammalian visual system, early stages of visual form perception begin with orientation selective neurons in primary visual cortex (V1). In many species (including humans, monkeys, tree shrews, cats, and ferrets), these neurons are organized in pinwheel-like orientation columns. To study the functional organization within orientation ...
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植物科学

Safe DNA-extraction Protocol Suitable for Studying Tree-fungus Interactions 适于研究树-真菌互作的安全DNA提取方法 作者:Susanna Keriö, Eeva Terhonen and Jared M. LeBoldus日期:06/05/2020,浏览量:1677,Q&A: 0
We present a safe and low-cost method suitable for DNA extraction from mycelium and tree tissue samples. After sample preparation, the extraction takes about 60 min. Method performance was tested by extracting DNA from various tree tissue samples and from mycelium grown on solid and liquid media. DNA was extracted from juvenile and mature host ...
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Determination of Ureides Content in Plant Tissues 植物组织中酰脲含量的测定 作者:Ignacio Lescano日期:06/05/2020,浏览量:1008,Q&A: 0
The ureides allantoin and allantoate are the main organic nitrogen compounds transported in several legumes, predominantly from N2 fixation. Moreover, recent studies point out a remarkable role for allantoin during several stress responses of plants other than legumes. The goal of this protocol is to determine ureides concentration in ...
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