• 卷册 10, 2020
  • 卷册 9, 2019
  • 卷册 8, 2018
  • 卷册 7, 2017
  • 卷册 6, 2016
  • 卷册 5, 2015
  • 卷册 4, 2014
  • 卷册 3, 2013
  • 卷册 2, 2012
  • 卷册 1, 2011
Image Credit: BioProtoc.3681

往期刊物(2020)

卷册: 10 期号: 14

生物化学

Immunofluorescent Staining of Claudin-2 in Cultured Kidney Tubular Cells 肾脏肾小管培养细胞中Claudin-2的免疫荧光染色 作者:Shaista Anwer and Katalin Szaszi日期:07/20/2020,浏览量:606,Q&A: 0
Members of the claudin family of tight junction proteins regulate paracellular permeability and modulate cell signaling. During junction remodeling, these proteins are selectively inserted into or retrieved from the tight junctions, but the control and coordination of these processes remain incompletely understood. Visualization of claudins allows ...
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生物物理学

Production and Isolation of Magnetic Protein Crystals in HEK293T Cells HEK293T细胞中磁性蛋白晶体的生产与分离 作者:Thomas L. Li and Bianxiao Cui日期:07/20/2020,浏览量:722,Q&A: 0
Advances in protein engineering have enabled the production of self-assembled protein crystals within living cells. Our recent publication demonstrates the production of ftn-PAK4, which is a ferritin-containing crystal that can mineralize iron and become magnetic when isolated. We have developed an optimized protocol for the production and ...
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Preparation and Characterization of Ginger Lipid-derived Nanoparticles for Colon-targeted siRNA Delivery 用于向结肠靶向siRNA递送的生姜脂质纳米粒的制备与表征 作者:Junsik Sung, Chunhua Yang, James F. Collins and Didier Merlin日期:07/20/2020,浏览量:523,Q&A: 0
Synthetic nanoparticle-based drug delivery system is widely known for its ability to increase the efficacy and specificity of loaded drugs, but it often suffers from relatively higher immunotoxicity and higher costs as compared to traditional drug formulations. Contrarily, plant-derived nanoparticles appear to be free from these limitations of ...
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癌症生物学

A CRISPR Competition Assay to Identify Cancer Genetic Dependencies 基于CRISPR竞争法鉴定癌症的遗传依赖性 作者:Vishruth Girish and Jason M. Sheltzer日期:07/20/2020,浏览量:1505,Q&A: 0
The CRISPR/Cas9 system is a powerful tool for genome editing, wherein the RNA-guided nuclease Cas9 can be directed to introduce double-stranded breaks (DSBs) at a targeted locus. In mammalian cells, these DSBs are typically repaired through error-prone processes, resulting in insertions or deletions (indels) at the targeted locus. Researchers can ...
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发育生物学

Isolation, Culture, and Differentiation of Primary Myoblasts Derived from Muscle Satellite Cells 肌卫星细胞原代成肌细胞的分离、培养和分化 作者:Kun Ho Kim, Jiamin Qiu and Shihuan Kuang日期:07/20/2020,浏览量:741,Q&A: 0
The skeletal muscle is key for body mobility and motor performance, but aging and diseases often lead to progressive loss of muscle mass due to wasting or degeneration of muscle cells. Muscle satellite cells (MuSCs) represent a population of tissue stem cells residing in the skeletal muscles and are responsible for homeostatic maintenance and ...
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In vitro Induction and Detection of Acrosomal Exocytosis in Human Spermatozoa 人精子中顶体胞吐作用的体外诱导和检测 作者:Shenae L. Cafe, Amanda L. Anderson and Brett Nixon日期:07/20/2020,浏览量:522,Q&A: 0
The acrosome reaction is a highly regulated exocytotic event that primes spermatozoa for successful fertilization. Upon induction, acrosomal exocytosis proceeds via a wave of vesiculation that radiates across the sperm head, destabilizing the acrosomal vesicle and resulting in the release of the acrosomal contents. Having shed their acrosome, ...
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免疫学

Metabolomic and Lipidomic Analysis of Bone Marrow Derived Macrophages 骨髓衍生巨噬细胞的代谢组学和脂质组学分析 作者:Gretchen L. Seim, Steven V. John and Jing Fan日期:07/20/2020,浏览量:888,Q&A: 0
Macrophages are highly plastic immune cells that are capable of adopting a wide array of functional phenotypes in response to environmental stimuli. The changes in macrophage function are often supported and regulated by changes in cellular metabolism. Capturing a comprehensive picture of metabolism is vital for understanding the role of metabolic ...
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微生物学

Intestinal Enteroid Culture for Human Astroviruses 人类星状病毒的肠样培养 作者:Irene A. Owusu, Carmen Mirabelli and Abimbola O. Kolawole日期:07/20/2020,浏览量:547,Q&A: 0
Human astroviruses (HAstV) are non-enveloped, positive-sense single stranded RNA viruses that typically cause gastroenteritis in children, the elderly and among immunocompromised individuals. Some HAstV species have also been implicated in neurological diseases. It is important to study these viruses to understand the pathogenesis and develop ...
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A SsrA/NIa-based Strategy for Post-Translational Regulation of Protein Levels in Gram-negative Bacteria 基于SsrA/NIa的革兰阴性菌蛋白质水平翻译后调控策略 作者:Gonzalo Durante-Rodríguez, Belén Calles, Víctor de Lorenzo and Pablo I. Nikel日期:07/20/2020,浏览量:614,Q&A: 0
Strategies to control the levels of key enzymes of bacterial metabolism are commonly based on the manipulation of gene of interest within the target pathway. The development of new protocols towards the manipulation of biochemical processes is still a major challenge in the field of metabolic engineering. On this background, the FENIX (functional ...
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神经科学

Method for Prolonged Incubation of Brain Slices 脑片长时间孵育方法的研究 作者:Orsolya Kekesi and Yossi Buskila日期:07/20/2020,浏览量:579,Q&A: 0
Slices of neuronal tissue maintain a high degree of topographical and functional properties of neurons and glia and therefore are extensively used for measurements of neuronal activity at the molecular, cellular and network levels. However, the lifespan of slice preparations is narrow, averaging of 6-8 hours. Moreover, the average viability of ...
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Calcein Release Assay to Measure Membrane Permeabilization by Recombinant Alpha-Synuclein 钙黄绿素释放法测定重组α-突触核蛋白膜通透性 作者:Sayan Dutta, Ben G. Watson, Seema Mattoo and Jean-Christophe Rochet日期:07/20/2020,浏览量:797,Q&A: 0
Lipid membranes are involved in regulating biochemical and biological processes and in modulating the selective permeability of cells, organelles, and vesicles. Membrane composition, charge, curvature, and fluidity all have concerted effects on cellular signaling and homeostasis. The ability to prepare artificial lipid assemblies that mimic ...
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植物科学

RNA Stability Measurements Using RT-qPCR in Arabidopsis Seedlings RT-qPCR法测定拟南芥幼苗RNA稳定性 作者:Tianran Jia and Brandon H. Le日期:07/20/2020,浏览量:581,Q&A: 0
Steady-state mRNA levels are determined by both the rates of transcription and degradation. Regulation of mRNA stability and/or degradation are key factors that can significantly affect mRNA levels and its biological functions. mRNA stability can be measured indirectly after transcription inhibition. This protocol described a rapid and sensitive ...
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Gene Expression and Genome Editing Systems by Direct Delivery of Macromolecules Into Rice Egg Cells and Zygotes 大分子直接导入水稻卵细胞和受精卵的基因表达和基因组编辑系统 作者:Erika Toda and Takashi Okamoto日期:07/20/2020,浏览量:662,Q&A: 0
Polyethylene glycol calcium (PEG-Ca2+)-mediated transfection allows rapid and efficient examination to analyze diverse cellular functions of genes of interest. In plant cells, macromolecules, such as DNA, RNA and protein, are delivered into protoplasts derived from somatic tissues or calli via PEG-Ca2+ transfection. To ...
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Generation and in Planta Functional Analysis of Potato Virus Y mutants 马铃薯Y病毒突变体的产生及其在植物中的功能分析 作者:Katja Stare, Anna Coll, Ion Gutiérrez-Aguirre, Magda Tušek Žnidarič, Maja Ravnikar, Andreja Kežar, Luka Kavčič, Marjetka Podobnik and Kristina Gruden日期:07/20/2020,浏览量:569,Q&A: 0
Potato virus Y (PVY), the type member of the genus Potyvirus (family Potyviridae), is the most widespread virus affecting potato and is included in the top five most economically detrimental plant viruses. Recently, the structure of the PVY virion has been determined by cryo-electron microscopy, which has opened the doors to ...
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系统生物学

HoSeIn: A Workflow for Integrating Various Homology Search Results from Metagenomic and Metatranscriptomic Sequence Datasets HoSeIn:整合来自宏基因组和元转录组序列数据集的各种同源性搜索结果的工作流 作者:Gaston Rozadilla, Jorgelina Moreiras Clemente and Christina B. McCarthy日期:07/20/2020,浏览量:624,Q&A: 0
Data generated by metagenomic and metatranscriptomic experiments is both enormous and inherently noisy. When using taxonomy-dependent alignment-based methods to classify and label reads, the first step consists in performing homology searches against sequence databases. To obtain the most information from the samples, nucleotide sequences are ...
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