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Image Credit: BioProtoc.3763


A Workflow for Ultra-rapid Analysis of Histone Post-translational Modifications with Direct-injection Mass Spectrometry 使用直接进样质谱对组蛋白翻译后修饰进行超快速分析的工作流程 作者:Natarajan V. Bhanu, Simone Sidoli and Benjamin A Garcia日期:09/20/2020,浏览量:2685,Q&A: 0
Chromatin modifications, like histone post translational modifications (PTMs), are critical for tuning gene expression and many other aspects of cell phenotype. Liquid chromatography coupled to mass spectrometry (LC-MS) has become the most suitable method to analyze histones and histone PTMs in a large-scale manner. Selected histone PTMs have ...
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Novel Protein-oligonucleotide Conjugation Method Involving a High-affinity Capture HaloTag 一种高亲和力捕获卤代烷脱氢酶标记的蛋白质寡核苷酸偶联新方法 作者:Junshi Yazaki日期:09/20/2020,浏览量:2409,Q&A: 0
Highly sensitive quantitative protein profiling can play a key role in the early diagnosis of diseases, such as autoimmune diseases and cancer. We developed a modified protein-oligonucleotide conjugation method termed HaloTag-mediated barcoding, for quantifying protein molecules at a higher sensitivity than conventional protein quantification ...
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Quantification of the Surface Expression of G Protein-coupled Receptors Using Intact Live-cell Radioligand Binding Assays 利用完整活细胞放射配体结合分析定量G蛋白偶联受体的表面表达 作者:Xin Xu and Guangyu Wu日期:09/20/2020,浏览量:2287,Q&A: 0
G protein-coupled receptors (GPCRs) are the most structurally diverse family of signaling proteins and regulate a variety of cell function. For most GPCRs, the cell surface is their functional destination where they are able to respond a wide range of extracellular stimuli, leading to the activation of intracellular signal transduction cascades. ...
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A Method to Efficiently Cryopreserve Mammalian Cells on Paper Platforms 一种纸质平台高效冷冻保存哺乳动物细胞的方法 作者:Muhammedin Deliorman, Pavithra Sukumar, Roaa Alnemari and Mohammad A. Qasaimeh日期:09/20/2020,浏览量:2230,Q&A: 0
This protocol describes a simple method to cryopreserve mammalian cells within filter papers as an alternative to conventional slow-freezing approach. The method involves treating paper fibers with fibronectin, using low concentrations of the cryoprotectant dimethyl sulfoxide (DMSO), and slow freezing cells to -80 °C at a 1 °C min-1 ...
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Immunoprecipitation of Acetyl-lysine and Western Blotting of Long-chain acyl-CoA Dehydrogenases and Beta-hydroxyacyl-CoA Dehydrogenase in Palmitic Acid Treated Human Renal Tubular Epithelial Cells 棕榈酸治疗的人肾小管上皮细胞中乙酰赖氨酸的免疫沉淀和长链酰基辅酶A脱氢酶和β-羟酰基辅酶A脱氢酶的蛋白质印迹 作者:Tingting Lv, Suwei Zhu, Yuan Ma, Hong Feng and Qiang Wan日期:09/20/2020,浏览量:2183,Q&A: 0
As one of the main energy metabolism organs, kidney has been proved to have high energy requirements and are more inclined to fatty acid metabolism as the main energy source. Long-chain acyl-CoA dehydrogenases (LCAD) and beta-hydroxyacyl-CoA dehydrogenase (beta-HAD), key enzymes involved in fatty acid oxidation, has been identified as the ...
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Production and Bioassay of a Diffusible Factor that Induces Gametophyte-to-Sporophyte Developmental Reprogramming in the Brown Alga Ectocarpus 水云属褐藻配子体-孢子体发育重编程扩散因子的产生及生物测定 作者:Haiqin Yao, Delphine Scornet, Yacine Badis, Akira F. Peters, Murielle Jam, Cecile Hervé, Philippe Potin, Susana M. Coelho and J. Mark Cock日期:09/20/2020,浏览量:2065,Q&A: 0
The brown alga Ectocarpus has a haploid-diploid life cycle that involves alternation between two multicellular generations, the sporophyte and the gametophyte. Life cycle generation is not determined by ploidy but by a genetic system that includes two different three amino acid loop extension homeodomain transcription factors called ...
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Immunohistochemistry of Kidney a-SMA, Collagen 1, and Collagen 3, in A Novel Mouse Model of Reno-cardiac Syndrome 心肾综合征新型小鼠模型中肾脏a-SMA,胶原1和胶原3的免疫组化研究 作者:Julius E. Kieswich, Jianmin Chen, Samira Alliouachene, Paul W. Caton, Kieran McCafferty, Christoph Thiemermann and Muhammad M. Yaqoob日期:09/20/2020,浏览量:1362,Q&A: 0
Cardiorenal syndrome defines a synergistic pathology of the heart and kidneys where failure of one organ causes failure in the other. The incidence of cardiovascular mortality caused by this syndrome, is 20 fold higher in the end stage renal disease (ESRD) population compared to the population as a whole thus necessitating the need for improved ...
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Surgical Induction of Endometriosis in Female Mice 雌性小鼠子宫内膜异位症的手术诱导 作者:Alejandra Escudero-Lara, David Cabañero and Rafael Maldonado日期:09/20/2020,浏览量:1629,Q&A: 0
Endometriosis is a common gynecological disease characterized by the presence of endometrial tissue outside the uterine cavity. It is frequently associated with pain, infertility and a reduced quality of life, and it lacks adequate treatment. Several rodent models of endometriosis have been developed through heterologous and homologous ...
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Site-specific Labeling of B Cell Receptor and Soluble Immunoglobulin B细胞受体和可溶性免疫球蛋白的定位标记 作者:Yu Wang, Zhixun Shen, Zhengpeng Wan and Wanli Liu日期:09/20/2020,浏览量:2496,Q&A: 0
B lymphocyte activation is regulated by its membrane-bound B cell receptors (BCRs) upon recognizing diverse antigens. It is hypothesized that antigen binding would trigger conformational changes within BCRs, followed by a series of downstream signaling activation. To measure the BCR conformational changes in live cells, a fluorescent site-specific ...
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Whole-genome Identification of Transcriptional Start Sites by Differential RNA-seq in Bacteria 细菌转录起始位点的差异RNA序列全基因组鉴定 作者:Ramón Cervantes-Rivera and Andrea Puhar日期:09/20/2020,浏览量:2956,Q&A: 0
Gene transcription in bacteria often starts some nucleotides upstream of the start codon. Identifying the specific Transcriptional Start Site (TSS) is essential for genetic manipulation, as in many cases upstream of the start codon there are sequence elements that are involved in gene expression regulation. Taken into account the classical gene ...
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Evaluation of Viable Cells in Pseudomonas aeruginosa Biofilms by Colony Count and Live/Dead Staining 菌落计数和活/死染色法评价铜绿假单胞菌生物膜中的活细胞 作者:Magdalena Pezzoni, Ramón Augusto Pizarro and Cristina Susana Costa日期:09/20/2020,浏览量:2240,Q&A: 0
Pseudomonas aeruginosa is a human pathogen capable to form robust biofilms. P. aeruginosa biofilms represent a serious problem because of the adverse effects on human health and industry, from sanitary and economic points of view. Typical strategies to break down biofilms have been long used, such as the use of disinfectants or ...
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Determination of Intracellular Ca2+ Concentration in the Human Pathogens Trypanosomatids Leishmania mexicana and Trypanosoma cruzi by the Use of the Fluorescent Ca2+ Indicator Fura-2 利用荧光Ca2+指示剂Fura-2测定人类病原体锥虫,墨西哥利什曼原虫和克氏锥虫中细胞内Ca2+的浓度 作者:Andre Rey-Cibati, Mariana Valladares-Delgado and Gustavo Benaim日期:09/20/2020,浏览量:1714,Q&A: 0
Ca2+ is an essential signaling messenger in all eukariotic cells, playing a pivotal role in many cellular functions as cell growth control (differentiation, fertilization and apoptosis), secretion, gene expression, enzyme regulation, among many others. This basic premise includes trypanosomatids as Trypanosoma cruzi and various ...
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Probe-Seq: Method for RNA Sequencing of Specific Cell Types from Animal Tissue 探针序列:动物组织中特定细胞类型的RNA测序方法 作者:Ryoji Amamoto, Mauricio D. Garcia, Emma R. West, Jiho Choi, Sylvain W. Lapan, Elizabeth A. Lane, Norbert Perrimon and Constance L. Cepko日期:09/20/2020,浏览量:2965,Q&A: 0
Most organs and tissues are composed of many types of cells. To characterize cellular state, various transcription profiling approaches are currently available, including whole-tissue bulk RNA sequencing, single cell RNA sequencing (scRNA-Seq), and cell type-specific RNA sequencing. What is missing in this repertoire is a simple, versatile method ...
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Long-term Devocalization of Zebra Finches 斑马雀的长期清音化研究 作者:Yunbok Kim, Daisuke Mizuguchi and Satoshi Kojima日期:09/20/2020,浏览量:2001,Q&A: 0
Songbirds, such as the zebra finch, are a popular animal model for studying the neural basis of vocal and complex skill learning. Adult male zebra finches produce courtship song toward females (referred to as ‘directed song’) and recording and analyzing sounds of directed song along with underlying neural activity is important for investigating ...
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In vitro AMPylation/Adenylylation of Alpha-synuclein by HYPE/FICD HYPE/FICD法体外氨酰化/腺苷酰化α-突触核蛋白 作者:Ali Camara, Anwesha Sanyal, Sayan Dutta, Jean-Christophe Rochet and Seema Mattoo日期:09/20/2020,浏览量:2018,Q&A: 0
One of the major histopathological hallmarks of Parkinson’s disease are Lewy bodies (LBs) –cytoplasmic inclusions, enriched with fibrillar forms of the presynaptic protein alpha-synuclein (α-syn). Progressive deposition of α-syn into LBs is enabled by its propensity to fibrillize into insoluble aggregates. We recently described a marked reduction ...
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Expression and Purification of Arabidopsis Transmembrane Protein BCM1 in Saccharomyces cerevisiae 拟南芥跨膜蛋白BCM1在酿酒酵母中的表达与纯化 作者:Peng Wang and Bernhard Grimm日期:09/20/2020,浏览量:1985,Q&A: 0
Heterologous expression and purification of transmembrane proteins have remained a challenge for decades hampering detailed biochemical and structural characterization of key enzymes and their interacting regulators in multiple metabolic pathways. An in-depth study on the newly identified Arabidopsis thaliana integral membrane protein ...
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Isolation and ex vivo Expansion of Human Limbal Epithelial Progenitor Cells 人角膜缘上皮祖细胞的分离及体外扩增 作者:Naresh Polisetti, Günther Schlunck, Thomas Reinhard, Friedrich E. Kruse and Ursula Schlötzer-Schrehardt日期:09/20/2020,浏览量:2422,Q&A: 0
Limbal stem cell transplantation has been used successfully to treat patients with limbal stem cell deficiency all over the world. However, long term clinical results often proved less satisfactory due to the low quality of the graft or inadequate properties of transplanted cells. To enhance the ex vivo expansion of human limbal ...
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In vitro Differentiation of Human iPSC-derived Cardiovascular Progenitor Cells (iPSC-CVPCs) 人iPSC衍生心血管祖细胞(iPSC-CVPCs)的体外分化 作者:Agnieszka D’Antonio-Chronowska, Matteo D’Antonio and Kelly A. Frazer日期:09/20/2020,浏览量:3704,Q&A: 0
Induced pluripotent stem cell derived cardiovascular progenitor cells (iPSC-CVPCs) provide an unprecedented platform for examining the molecular underpinnings of cardiac development and disease etiology, but also have great potential to play pivotal roles in the future of regenerative medicine and pharmacogenomic studies. Biobanks like iPSCORE ( ...
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