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Image Credit: BioProtoc.3783


卷册: 10 期号: 19


Assembly and Imaging Set up of PIE-Scope PIE-Scope的组装和成像设置 作者:Sergey Gorelick, David A. Dierickx, Genevieve Buckley, James C. Whisstock and Alex de Marco日期:10/05/2020,浏览量:296,Q&A: 0
Cryo-Electron Tomography (cryo-ET) is a method that enables resolving the structure of macromolecular complexes directly in the cellular environment. However, sample preparation for in situ Cryo-ET is labour-intensive and can require both cryo-lamella preparation through cryo-Focused Ion Beam (FIB) milling and correlative light microscopy ...
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A Novel and Robust Single-cell Trapping Method on Digital Microfluidics 一种新颖、可靠的数字微流控单细胞捕获方法 作者:Jiao Zhai, Haoran Li, Ada Hang-Heng Wong, Cheng Dong, Shuhong Yi, Yanwei Jia, Pui-In Mak, Chuxia Deng and Rui Martins日期:10/05/2020,浏览量:263,Q&A: 0
Due to cell heterogeneity, the differences among individual cells are averaged out in bulk analysis methods, especially in the analysis of primary tumor biopsy samples from patients. To deeply understand the cell-to-cell variation in a primary tumor, single-cell culture and analysis with limited amount of cells are in high demand. Microfluidics ...
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Affinity Purification of GO-Matryoshka Biosensors from E. coli for Quantitative Ratiometric Fluorescence Analyses 用于定量比率荧光分析的大肠杆菌GO-Matryoshka生物传感器亲和纯化 作者:Mayuri Sadoine, Vanessa Castro-Rodríguez, Tobias Poloczek, Helene Javot, Erdem Sunal, Michael M. Wudick and Wolf B. Frommer日期:10/05/2020,浏览量:360,Q&A: 0
Genetically encoded biosensors are powerful tools for quantitative visualization of ions and metabolites in vivo. Design and optimization of such biosensors typically require analyses of large numbers of variants. Sensor properties determined in vitro such as substrate specificity, affinity, response range, dynamic range, and ...
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Double Labeling of PDGFR-β and α-SMA in Swine Models of Acute Kidney Injury to Detect Pericyte-to-Myofibroblast Transdifferentation as Early Marker of Fibrosis 双标记PDGFR-β和α-SMA在猪急性肾损伤模型中检测周细胞-肌成纤维细胞转化作为早期纤维化标志物的研究 作者:Alessandra Stasi, Rossana Franzin, Chiara Divella, Loreto Gesualdo, Giovanni Stallone and Giuseppe Castellano日期:10/05/2020,浏览量:219,Q&A: 0
Growing evidences suggest that peritubular capillaries pericytes are the main source of scar-forming myofibroblasts during chronic kidney disease (CKD), as well as early phases of acute kidney injury (AKI). In a swine model of sepsis and I/R (Ischemia Reperfusion) injury-induced AKI we demonstrated that renal pericytes are able to ...
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Fluorescence Measurement and Calibration of Intracellular pH in Starfish Oocytes 海星卵母细胞内pH值的荧光测定与校准 作者:Enako Hosoda and Kazuyoshi Chiba日期:10/05/2020,浏览量:231,Q&A: 0
Oocyte maturation is a process wherein an oocyte arrested at prophase of meiosis I resumes meiosis to become a fertilizable egg. In starfish ovaries, a hormone released from follicle cells activates the oocytes, resulting in an increase in their intracellular pH (pHi), which is required for spindle assembly. Herein, we describe a ...
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Radioactive Assay of in vitro Glutamylation Activity of the Legionella pneumophila Effector Protein SidJ 嗜肺军团菌效应蛋白SidJ体外谷氨酰化活性的放射性测定 作者:Alan G. Sulpizio, Jung-Ho Shin, Marena E. Minelli and Yuxin Mao日期:10/05/2020,浏览量:297,Q&A: 0
The Legionella effector protein SidJ has recently been identified to perform polyglutamylation on another Legionella effector, SdeA, ablating SdeA’s activity. SidJ is a kinase-like protein that requires the small eukaryotic protein calmodulin to perform glutamylation. Glutamylation is a relatively uncommon type of ...
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TetR Regulated in vivo Repression Technology to Identify Conditional Gene Silencing in Genetically Engineerable Bacteria Using Vibrio cholerae Murine Infections as Model System 以小鼠霍乱弧菌感染为模型系统的TetR调控体内抑制技术鉴定基因工程菌的条件基因沉默 作者:Franz G. Zingl, Fabian Mitterer, Himadri B. Thapa and Stefan Schild日期:10/05/2020,浏览量:219,Q&A: 0
Investigation of bacterial gene regulation upon environmental changes is still a challenging task. For example, Vibrio cholerae, a pathogen of the human gastrointestinal tract, faces diverse transient conditions in different compartments upon oral ingestion. Genetic reporter systems have been demonstrated to be extremely powerful tools to ...
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Analysis of Gram-negative Bacteria Peptidoglycan by Ultra-performance Liquid Chromatography 革兰阴性菌肽聚糖的超高效液相色谱法分析 作者:Laura Alvarez, Baptiste Cordier, Sven van Teeffelen and Felipe Cava日期:10/05/2020,浏览量:298,Q&A: 0
Bacteria are surrounded by a protective peptidoglycan cell wall. Provided that this structure and the enzymes involved are the preferred target for our most successful antibiotics, determining its structural and chemical complexity is of the highest interest. Traditionally, high-performance liquid chromatography (HPLC) analyses have been ...
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Simple Time-lapse Imaging for Quantifying the Hydrostatic Production of Oxygenic Photogranules 用于定量含氧光颗粒静水压力的简单时差成像 作者:Esmee D. Joosten, Jérôme Hamelin and Kim Milferstedt日期:10/05/2020,浏览量:307,Q&A: 0
Oxygenic photogranules (OPGs) are dense, three-dimensional aggregates containing a syntrophic, light-driven microbial community. Their temporal and spatial development interests microbial ecologists working at the bioprocess engineering interface, as this knowledge can be used to optimize biotechnological applications, such as wastewater treatment ...
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Using RNA Sequencing and Spike-in RNAs to Measure Intracellular Abundance of lncRNAs and mRNAs 应用RNA测序和RNAs峰值检测lncRNAs和mRNAs的细胞内丰度 作者:Megan D. Schertzer, McKenzie M. Murvin and J. Mauro Calabrese日期:10/05/2020,浏览量:250,Q&A: 0
Long noncoding RNAs (lncRNAs) play essential roles in normal physiology and in disease but their mechanisms of action can be challenging to identify. For mechanistic studies, it is often useful to know a lncRNA’s intracellular abundance, i.e., approximately how many molecules of the lncRNA are present in a typical cell of a cell-type of ...
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Preparing Viable Hippocampal Slices from Adult Mice for the Study of Sharp Wave-ripples 成年小鼠海马脑片的制备及其锐波波纹的研究 作者:Linhua Liu, Xiaojing Zhou and Jian-young Wu日期:10/05/2020,浏览量:227,Q&A: 0
We describe a protocol for preparing acute brain slices which can produce robust hippocampal sharp wave-ripples (SWRs) in vitro. The protocol is optimized for its simplicity and reliability for the preparation of solutions, slicing, and recovery incubation. Most slices in almost every mouse prepared though the protocol expressed vigorous ...
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An Operant Conditioning Model Combined with a Chemogenetic Approach to Study the Neurobiology of Food Addiction in Mice 操作式条件反射模型结合化学遗传学方法研究小鼠食物成瘾的神经生物学机制 作者:Elena Martín-García, Laura Domingo-Rodriguez and Rafael Maldonado日期:10/05/2020,浏览量:287,Q&A: 0
The study of food addiction comprises 3 hallmarks that include the persistence to response without an outcome, the strong motivation for palatable food, and the loss of inhibitory control over food intake that leads to compulsive behavior in addicted individuals. The complex multifactorial nature of this disorder and the unknown neurobiological ...
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A Protocol for Flavonols, Kaempferol and Quercetin, Staining in Plant Root Tips 植物根尖中黄酮醇、山奈酚和槲皮素的染色方法 作者:Nguyen Hoai Nguyen日期:10/05/2020,浏览量:390,Q&A: 0
Flavonols are a subclass of flavonoids of the group of plant secondary metabolites. In planta, flavonols play various functions such as antioxidant and natural regulator of auxin polar transport. Many lines of evidence have shown that flavonols also contribute to human health in anti-oxidation, anti-inflammation, and even prevention some ...
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Multitarget Immunohistochemistry for Confocal and Super-resolution Imaging of Plant Cell Wall Polysaccharides 植物细胞壁多糖共聚焦和超分辨成像的多靶点免疫组织化学研究 作者:Kalina T. Haas, Methieu Rivière, Raymond Wightman and Alexis Peaucelle日期:10/05/2020,浏览量:607,Q&A: 0
The plant cell wall (PCW) is a pecto-cellulosic extracellular matrix that envelopes the plant cell. By integrating extra-and intra-cellular cues, PCW mediates a plethora of essential physiological functions. Notably, it permits controlled and oriented tissue growth by tuning its local mechano-chemical properties. To refine our knowledge of these ...
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Fluidigm Based Single-cell Gene Expression Library Preparation from Patient-derived Small Intestinal Organoids 基于flifgm的源于患者小肠类器官单细胞基因表达文库的制备 作者:Kohei Suzuki and Ryuichi Okamoto日期:10/05/2020,浏览量:239,Q&A: 0
In this protocol, we describe our methods to isolate crypts from patients' biopsy samples and to culture human intestinal stem cells as it’s called “organoid.” Beyond that, we describe how to dissociate organoids cells into single cells for single-cell analysis as a further application. This protocol should provide investigators sufficient tools ...
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