• 卷册 11, 2021
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Image Credit: BioProtoc.3823

生物化学

Total Triglyceride Quantification in Caenorhabditis elegans 秀丽隐杆线虫总甘油三酯的定量研究 作者:Anjali Sandhu and Varsha Singh日期:11/20/2020,浏览量:1904,Q&A: 0
Several studies suggest an important role of lipid metabolism in regulating longevity of Caenorhabditis elegans. Therefore, assays to quantify lipids have enormous value in understanding aging and pathologies associated with it. Approximately 70% of lipid metabolism genes in the nematode have orthologs in humans. Amenability of C. elegans ...
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Cleavable Affinity Purification (Cl-AP): A One-step Procedure to Affinity Purify Protein Complexes CI-AP:一种亲和纯化蛋白质复合物的一步法 作者:Ammarah Tariq, Lucy Green, Christian Soeller and James G. Wakefield日期:11/20/2020,浏览量:1908,Q&A: 0

Cleavable Affinity Purification (Cl-AP) uses a tripartite system of Protein-A-Streptavidin beads and nanobodies, coupled with a biotinylated, thiol-cleavable linker, providing one-step affinity purification from lysates of tissues expressing tagged proteins. This technique allows fluorescent versions of mitotic protein complexes to be isolated

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生物物理学

Serial Cryomicrotomy of Saccharomyces cerevisiae for Serial Electron Cryotomography 用于连续电子低温断层扫描的酿酒酵母连续冷冻切片 作者:Cai Tong Ng, Mark S. Ladinsky and Lu Gan日期:11/20/2020,浏览量:1995,Q&A: 0
Electron cryotomography (cryo-ET) is an increasingly popular technique to study cellular structures and macromolecules in situ. Due to poor penetration of electrons through thick biological samples, the vitreously frozen samples for cryo-ET need to be thin. For frozen-hydrated cells, such samples can be produced either by cryomicrotomy or ...
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癌症生物学

Dissecting the Rat Mammary Gland: Isolation, Characterization, and Culture of Purified Mammary Epithelial Cells and Fibroblasts 解剖大鼠乳腺:纯化的乳腺上皮细胞和成纤维细胞的分离,鉴定和培养 作者:Elizabeth A. Tovar, Rachael Sheridan, Curt J. Essenburg, Patrick S. Dischinger, Menusha Arumugam, Megan E. Callaghan, Carrie R. Graveel and Matthew R. Steensma日期:11/20/2020,浏览量:2095,Q&A: 1

With the advent of CRISPR-Cas and the ability to easily modify the genome of diverse organisms, rat models are being increasingly developed to interrogate the genetic events underlying mammary development and tumorigenesis. Protocols for the isolation and characterization of mammary epithelial cell subpopulations have been thoroughly developed for

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Magnet-assisted Flow Cytometry of in vivo Tumors to Quantitate Cell-specific Responses to Magnetic Iron Oxide Nanoparticles 磁辅助流式细胞术检测体内肿瘤细胞以量化对磁性氧化铁纳米粒子的特异性反应 作者:Preethi Korangath and Robert Ivkov日期:11/20/2020,浏览量:1407,Q&A: 0
A clear understanding of nanoparticle interactions with living systems at the cellular level is necessary for developing nanoparticle-based therapeutics. Magnetic iron oxide nanoparticles provide unique opportunities to study these interactions because of their responsiveness to magnetic fields. This enables sorting of cells containing ...
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免疫学

Maternal Immune Activation with the Viral Mimetic Poly:IC in Pregnant Rats Poly:IC模拟病毒感染对孕鼠的母体免疫激活 作者:Thaisa Meira Sandini, Quentin Greba, Brittney Rose Lins and John George Howland日期:11/20/2020,浏览量:1299,Q&A: 0

Maternal immune activation (MIA) is increasingly well appreciated as an environmental risk factor for some psychiatric disorders. Administration of proinflammatory compounds such as the synthetic double-stranded RNA molecule polyinosinic-polycytidylic acid (polyI:C) to pregnant rodents results in the release of proinflammatory cytokines in the

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Generating Three-dimensional Human Granulomas in vitro to Study Mycobacterium tuberculosis-host Interaction 通过体外生成三维人体肉芽肿研究结核分枝杆菌与宿主的相互作用 作者:Ainhoa Arbués, Michael Kammüller and Damien Portevin日期:11/20/2020,浏览量:1471,Q&A: 0
Granulomas are organized multicellular structures that constitute the hallmark of an infection by the human pathogen Mycobacterium tuberculosis (Mtb). A better understanding of the complex host-Mtb interactions within the granuloma’s environment may lead to new therapeutic or preventive tools to improve the control of the ...
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微生物学

Rapid Isolation and Purification of Secreted Bacteriocins from Streptococcus mutans and Other Lactic Acid Bacteria 变形链球菌及其他乳酸菌分泌细菌素的快速分离纯化 作者:Marcus Cheng, Siew-Ging Gong and Céline M. Lévesque日期:11/20/2020,浏览量:2112,Q&A: 0
Bacteriocins are small ribosomally synthesized antimicrobial peptides produced by some microorganisms including lactic acid bacteria (LAB), a group of Gram-positive bacteria (cocci, rods) expressing high tolerance for low pH. Bacteriocins kill bacteria rapidly and are biologically active at very low concentrations. Bacteriocins produced by LAB ...
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Giant Mimiviridae CsCl Purification Protocol 拟菌病毒科巨型病毒的CsCl纯化方案 作者:Lionel Bertaux, Audrey Lartigue and Sandra Jeudy日期:11/20/2020,浏览量:1113,Q&A: 0
While different giant viruses’ purification protocols are available, they are not fully described and they use sucrose gradient that does not reach an equilibrium. Here, we report a protocol for the purification of members of the Mimiviridae family virions resulting from Acanthamoeaba castellanii infections. Viruses are ...
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A Quick Method for Screening Biocontrol Efficacy of Bacterial Isolates against Bacterial Wilt Pathogen Ralstonia solanacearum in Tomato 细菌分离物对番茄青枯病病原菌青枯雷尔氏菌生防效果的快速筛选方法 作者:Heena Agarwal, Bhaskar Dowarah and Niraj Agarwala日期:11/20/2020,浏览量:2030,Q&A: 1

Ralstonia solanacearum is a bacterial phytopathogen able to cause bacterial wilt disease in more than 200 plant species. Plant disease biocontrol strategies are used for controlling this disease and tomato is used as a model plant to conduct R. solanacearum associated studies. Conventional screening methods such as seed bacterization, soil

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神经科学

In vitro Time-lapse Imaging of Primary Cilium in Migrating Neuroblasts 迁移神经母细胞中初级纤毛的体外延时成像 作者:Masato Sawada, Mami Matsumoto, Keishi Narita, Natsuko Kumamoto, Shinya Ugawa, Sen Takeda and Kazunobu Sawamoto日期:11/20/2020,浏览量:1461,Q&A: 0
Neuronal migration is a critical step for the development of neuronal circuits in the brain. Immature new neurons (neuroblasts) generated in the postnatal ventricular-subventricular zone (V-SVZ) show a remarkable potential to migrate for a long distance at a high speed in the postnatal mammalian brain, and are thus a powerful model to analyze the ...
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Confocal Microscopy of Reovirus Transport in Living Dorsal Root Ganglion Neurons 活体背根节神经元中呼肠孤病毒转运的共聚焦显微镜观察 作者:Pavithra Aravamudhan, Krishnan Raghunathan and Terence S. Dermody日期:11/20/2020,浏览量:1477,Q&A: 0

Neurotropic reoviruses repurpose host machinery to traffic over long distances in neuronal processes and access distal replication sites. Understanding mechanisms of neuronal transmission is facilitated by using simplified in vitro primary neuronal culture models. Advances in the design of compartmentalized microfluidic devices lend robustness to

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Visual-looming Shadow Task with in-vivo Calcium Activity Monitoring to Assess Defensive Behaviors in Mice 视觉变化阴影任务结合体内钙离子活性监测评估小鼠防御行为 作者:Nuria Daviu, Tamas Fuzesi, David G.Rosenegger, Govind Peringod, Kathryn Simone and Jaideep S. Bains日期:11/20/2020,浏览量:1544,Q&A: 0
There has been a clear movement in recent years towards the adoption of more naturalistic experimental regimes for the study of behavior and its underlying neural architecture. Here we provide a protocol that allows experimenters working with mice, to mimic a looming and advancing predatory threat from the sky. This approach is easy to implement ...
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植物科学

Agrobacterium-mediated Transformation of Sweet Basil (Ocimum basilicum) 农杆菌介导的罗勒(Ocimum basilicum)转化 作者:Natasha Navet and Miaoying Tian日期:11/20/2020,浏览量:2315,Q&A: 1

Sweet basil (Ocimum basilicum) is a popular herb with high economic value and is currently threatened by a severe oomycete disease. An efficient transformation method is a prerequisite for gene functional analysis to accelerate molecular breeding and deploy effective disease management strategies, and breeding through genetic engineering. Here we

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