• 卷册 11, 2021
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  • 卷册 5, 2015
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  • 卷册 3, 2013
  • 卷册 2, 2012
  • 卷册 1, 2011
Image Credit: BioProtoc.3873

往期刊物(2021)

卷册: 11 期号: 1

癌症生物学

A Rigorous Quantitative Approach to Analyzing Phagocytosis Assays 吞噬试验的严格定量分析方法 作者:Michael D. Caponegro, Kaitlyn Koenig Thompson, Maryam Tayyab and Stella E. Tsirka日期:01/05/2021,浏览量:1743,Q&A: 0
Studying monocytic cells in isolated systems in vitro contributes significantly to the understanding of innate immune physiology. Functional assays produce read outs which can be used to measure responses to selected stimuli, such as pathogen exposure, antigen loading, and cytokine stimulation. Integration of these results with high ...
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A Transient Transfection-based Cell Adhesion Assay with 293T Cells 基于瞬时转染的293T细胞粘附实验 作者:Rohit Singh and Beom K. Choi日期:01/05/2021,浏览量:998,Q&A: 0

The in vitro cell adhesion assay is a quantitative method for measuring selective cell adhesion to specific proteins. Traditionally, cell adhesion assays employ purified protein immobilized on a solid glass or plastic surface. Here, we describe a transient 293T cell transfection-based cell adhesion assay to study selective cell adhesion of a

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CENP-C Phosphorylation by CDK1 in vitro CDK1体外磷酸化CENP-C的研究 作者:Reito Watanabe, Masatoshi Hara, Mariko Ariyoshi and Tatsuo Fukagawa日期:01/05/2021,浏览量:847,Q&A: 0

Accurate chromosome segregation during mitosis requires the kinetochore, a large protein complex, which makes a linkage between chromosomes and spindle microtubes. An essential kinetochore component, CENP-C, is phosphorylated by Cyclin-B-Cyclin dependent kinase 1 (CDK1) that is a master kinase for mitotic progression, promoting proper kinetochore

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细胞生物学

A Simple Microplate Assay for Reactive Oxygen Species Generation and Rapid Cellular Protein Normalization 用于活性氧生成和快速细胞蛋白质标准化的简单微孔板检测 作者:Neville S. Ng and Lezanne Ooi日期:01/05/2021,浏览量:1482,Q&A: 0
2',7'-dichlorofluorescein (DCF) and derivatives are commonly used as fluorescent indicators of a broad spectrum of reactive oxygen species (ROS) generation in cell-based assays. However, there are numerous challenges inherent to the utilization of DCF probes for intracellular microscopic analysis, including bleaching and probe efflux. Plate ...
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A Parkinson’s Disease-relevant Mitochondrial and Neuronal Morphology High-throughput Screening Assay in LUHMES Cells LUHMES细胞中与帕金森氏病相关的线粒体和神经元形态学高通量筛选分析 作者:Tom Leah, Irina Vazquez-Villaseñor, Laura Ferraiuolo, Stephen B Wharton and Heather Mortiboys日期:01/05/2021,浏览量:1232,Q&A: 0

Parkinson’s disease is a devastating neurodegenerative disorder affecting 2-3% of the population over 65 years of age. There is currently no disease-modifying treatment. One of the predominant pathological features of Parkinson’s disease is mitochondrial dysfunction, and much work has aimed to identify therapeutic compounds which can

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发育生物学

Measuring Bone Volume at Multiple Densities by Micro-computed Tomography 显微ct测量多重密度骨体积 作者:Emma C. Walker, Narelle E. McGregor, Audrey S. M. Chan and Natalie A. Sims日期:01/05/2021,浏览量:1065,Q&A: 0

Bone strength is controlled by both bone mass, and the organization and quality of the bone material. The current standard method for measuring bone mass in mouse and rat studies is micro-computed tomography. This method typically uses a single threshold to identify bone material in the cortical and trabecular regions. However, this single

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微生物学

Assessment of Diadenylate Cyclase and c-di-AMP-phosphodiesterase Activities Using Thin-layer and Ion Exchange Chromatography 使用薄层和离子交换色谱评估二腺苷酸环化酶和c-di-AMP-磷酸二酯酶的活性 作者:Andreas Latoscha, David Jan Drexler, Gregor Witte and Natalia Tschowri日期:01/05/2021,浏览量:711,Q&A: 0

All living cells use cyclic nucleotides as second messengers for signal sensing and transduction. Cyclic di-3′,5′-adenosine monophosphate (c-di-AMP) is primarily involved in the control of bacterial and euryarcheal osmoadaptation and is produced by diadenylate cyclases from two molecules of ATP. Specific phosphodiesterases hydrolyze

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神经科学

Calcium Imaging of Neuronal Activity under Gradually Changing Odor Stimulation in Caenorhabditis elegans 渐变气味刺激下秀丽隐杆线虫神经元活动的钙成像 作者:Yuki Tanimoto and Koutarou D. Kimura日期:01/05/2021,浏览量:746,Q&A: 0

Olfactory behavior is among the most fundamental animal behaviors both in the wild and in the laboratory. To elucidate the neural mechanisms underlying olfactory behavior, it is critical to measure neural responses to odorant concentration changes resembling those that animals actually sense during olfactory behavior. However, reproducing the

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Optimized Immunostaining of Embryonic and Early Postnatal Mouse Brain Sections 胚胎和产后早期小鼠脑切片的优化免疫染色 作者:Kawssar Harb, Michele Bertacchi and Michèle Studer日期:01/05/2021,浏览量:1109,Q&A: 0

The mammalian neocortex, the outer layer of the cerebrum and most recently evolved brain region, is characterized by its unique areal and laminar organization. Distinct cortical layers and areas can be identified by the protein expression of graded transcription factors and molecular determinants that define the identity of different projection

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Establishing an Adult Mouse Brain Hippocampal Organotypic Slice Culture System that Allows for Tracing and Pharmacological Manipulation of ex vivo Neurogenesis 用于追踪和离体神经发生药理学操作的成年小鼠脑器官型海马切片培养系统的建立 作者:Steffen Mayerl and Charles ffrench-Constant日期:01/05/2021,浏览量:798,Q&A: 0

The function of the hippocampus depends on the process of adult hippocampal neurogenesis which underpins the exceptional neural plasticity of this structure, and is also frequently affected in CNS pathologies. Thus, manipulation of this process represents an important therapeutic goal. To identify potential strategies, organotypic adult brain

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Non-separate Mouse Sclerochoroid/RPE/Retina Staining and Whole Mount for the Integral Observation of Subretinal Layer 小鼠巩膜脉络膜/视网膜色素上皮/视网膜不分离染色及用于视网膜下层整体观察的全组织染色法 作者:Sung-Jin Lee and Soo-Young Kim日期:01/05/2021,浏览量:526,Q&A: 0

The subretinal layer between retinal pigment epithelium (RPE) and photoreceptors is a region involved in inflammation and angiogenesis during the procession of diseases such as age-related macular degeneration. The current protocols of whole mounts (retina and RPE) are unable to address the intact view of the subretinal layer because the

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植物科学

Non-radioactive Assay to Determine Product Profile of Short-chain Isoprenyl Diphosphate Synthases 用于确定短链异戊烯基二磷酸合酶产物谱的非放射性分析 作者:Avanish Rai and Dinesh A. Nagegowda日期:01/05/2021,浏览量:654,Q&A: 0
Isoprenoids represent the largest class of metabolites with amazing diversities in structure and function. They are involved in protecting plants against pathogens or herbivores or involved in attracting pollinators. Isoprenoids are derived from geranyl diphosphate (GPP; C10), farnesyl diphosphate (FPP; C15), geranylgeranyl ...
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Development and Standardization of Rapid and Efficient Seed Germination Protocol for Cannabis sativa 大麻种子快速高效发芽规程的建立与标准化 作者:Aleksei Sorokin, Narendra Singh Yadav, Daniel Gaudet and Igor Kovalchuk日期:01/05/2021,浏览量:871,Q&A: 0

Cannabis seed germination is an important process for growers and researchers alike. Many biotechnological applications require a reliable sterile method for seed germination. This protocol outlines a seed germination procedure for Cannabis sativa using a hydrogen peroxide (H2O2) solution as liquid germination media. In this protocol, all three

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Stable Transformation of Arabidopsis thaliana Cell Suspension Cultures: A Case Study for the Overexpression of The COI1 Receptor 拟南芥细胞悬浮培养的稳定转化:COI1受体过度表达的案例研究 作者:Eva K. Díaz-Sánchez, José A. O’Brien, Imma Pérez-Salamó, Jovaras Krasauskas, Moritz Bömer and Alessandra Devoto日期:01/05/2021,浏览量:1206,Q&A: 1
Cell suspension cultures have been studied for decades to produce natural molecules. However, the difficulty in generating stably transformed cell lines has limited their use to produce high value chemicals reproducibly and in elevated quantities.

In this protocol, a method to stably transform and maintain Arabidopsis cell ...
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A Pulse–chase EdU Method for Detection of Cell Division Orientation in Arabidopsis and Juncus prismatocarpus Leaf Primordia 脉冲追踪EdU法检测拟南芥和笄石菖叶原基细胞分裂方向 作者:Xiaofeng Yin and Hirokazu Tsukaya日期:01/05/2021,浏览量:677,Q&A: 0
In plants, the morphological diversity of leaves is largely determined by cell division, especially cell division orientation. Whereas cell division itself is easily monitored, the detection and quantification of cell division orientation are difficult. The few existing methods for detection and quantification of cell division orientation are ...
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Efficient Transient Gene Knock-down in Tobacco Plants Using Carbon Nanocarriers 应用碳纳米载体对烟草基因进行高效瞬时敲减 作者:Gozde S. Demirer and Markita P. Landry日期:01/05/2021,浏览量:1114,Q&A: 0
Gene knock-down in plants is a useful approach to study genotype-phenotype relationships, render disease resistance to crops, and enable efficient biosynthesis of molecules in plants. Small interfering RNA (siRNA)-mediated gene silencing is one of the most common ways to achieve gene knock-down in plants. Traditionally, siRNA is delivered into ...
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