• 卷册 11, 2021
  • 卷册 10, 2020
  • 卷册 9, 2019
  • 卷册 8, 2018
  • 卷册 7, 2017
  • 卷册 6, 2016
  • 卷册 5, 2015
  • 卷册 4, 2014
  • 卷册 3, 2013
  • 卷册 2, 2012
  • 卷册 1, 2011
Image Credit: BioProtoc.3907

往期刊物(2021)

卷册: 11 期号: 3

生物化学

Using 14C-acetate Pulse-chase Labeling to Study Fatty Acid and Glycerolipid Metabolism in Plant Leaves 用14C -乙酸酯脉冲追逐标记法研究植物叶片中脂肪酸和甘油脂代谢 作者:Linhui Yu, Chao Zhou, Jilian Fan and Changcheng Xu日期:02/05/2021,浏览量:1667,Q&A: 0

Lipids metabolism is comprised of networks of reactions occurred in different subcellular compartments. Isotopic labeling is a good way to track the transformations and movements of metabolites without perturbing overall cellular metabolism. Fatty acids, the building blocks of membrane lipids and storage triacylglycerols, are synthesized in

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Purification of Cytosolic Phospholipase A2α C2-domain after Expression in Soluble Form in Escherichia coli 可溶性磷脂酶A2αC2结构域在大肠杆菌中表达后的纯化 作者:Yoshinori Hirano, Yong-Guang Gao, Dhirendra K. Simanshu, Daniel J. Stephenson, Ngoc T. Vu, Lucy Malinina, Charles E. Chalfant, Dinshaw J. Patel and Rhoderick E. Brown日期:02/05/2021,浏览量:801,Q&A: 0

Previous expression/purification strategies for cytosolic phospholipase A2α C2-domain in Escherichia coli have relied on refolded protein recovered from inclusion bodies and sometimes containing C-terminal Cys139Ala and Cys141Ser substitutions to eliminate potential refolding complications induced by Cys residues. The protocol presented

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生物物理学

Resolving Structural Changes of Photoreceptors in Living Escherichia coli via In-cell Infrared Difference Spectroscopy 利用细胞内红外差谱技术分析活大肠杆菌光感受器的结构变化 作者:Lukas Goett-Zink, Jessica L. Klocke and Tilman Kottke日期:02/05/2021,浏览量:1961,Q&A: 0

Several in-cell spectroscopic techniques have been developed recently to investigate the structure and mechanism of proteins in their native environment. Conditions in vivo differ dramatically from those selected for in vitro experiments. Accordingly, the cellular environment can affect the protein mechanism for example by molecular crowding or

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癌症生物学

Carboxyfluorescein Dye Uptake to Measure Connexin-mediated Hemichannel Activity in Cultured Cells 用羧荧光素染料上染率测定培养细胞中连接蛋白介导的半通道活性 作者:Joe A. Potter, Gareth W. Price, Chelsy L. Cliff, Bethany M. Williams, Claire E. Hills and Paul E. Squires日期:02/05/2021,浏览量:2636,Q&A: 0

Connexins are membrane bound proteins that facilitate direct and local paracrine mediated cell-to-cell communication through their ability to oligomerise into hexameric hemichannels. When neighbouring channels align, they form gap-junctions that provide a direct route for information transfer between cells. In contrast to intact gap junctions,

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Isolation of Murine Primary Aortic Smooth Muscle Cells 小鼠主动脉平滑肌细胞的分离 作者:Max Ole Hubert, Juan Rodriguez-Vita, Lena Wiedmann and Andreas Fischer日期:02/05/2021,浏览量:2178,Q&A: 0

Vascular smooth muscle cells (VSMCs) have been cultured for decades to study the role of these cells in cardiovascular disorders. The most common source of VSMCs is the rat aorta. Here we show the adaptation of this method to isolate and culture mouse aortic VSMCs. The advantage of this method is that there are many more transgenic mouse lines

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发育生物学

Induction of Epithelial-mesenchymal Transition in MDCK II Cells 诱导MDCK II细胞上皮-间充质转化 作者:Agnieszka Pastuła and Richard Lundmark日期:02/05/2021,浏览量:921,Q&A: 0

Epithelial-mesenchymal transition (EMT) is a reversible process of epithelial cell transdifferentiation into a mesenchymal cell, that enables initiation of cell migration. EMT plays an important role in embryonic development, tissue repair and cancer metastasis. Better understanding of cellular and molecular events during EMT will not only provide

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Ecdysone Quantification from Whole Body Samples of Drosophila melanogaster Larvae 黑腹果蝇幼虫全身蜕皮激素的定量分析 作者:Takashi Koyama and Christen K. Mirth日期:02/05/2021,浏览量:984,Q&A: 0

Steroid hormones strictly control the timing of sexual maturation and final body size both in vertebrates and invertebrates. In insects, the steroid hormone ecdysone controls the timing of the molts between larval instars as well as the transition to metamorphosis. Growth during the final instar accounts for over 80% of the increase in final mass

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免疫学

A Versatile Protocol to Quantify BCR-mediated Phosphorylation in Human and Murine B Cell Subpopulations 量化人类和小鼠B细胞亚群中BCR介导的磷酸化的通用实验方法 作者:Jasper Rip, Rudi W. Hendriks and Odilia B. J. Corneth日期:02/05/2021,浏览量:929,Q&A: 0

Signal transduction is the process by which molecular signals are transmitted from the cell surface to its interior, resulting in functional changes inside the cell. B cell receptor (BCR) signaling is of crucial importance for B cells, as it regulates their differentiation, selection, survival, cellular activation and proliferation. Upon BCR

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In vitro STING Activation with the cGAMP-STINGΔTM Signaling Complex cGAMP-STINGΔTM信号复合物体外激活STING 作者:Yanpu He, Celestine Hong, Darrell J. Irvine, Jiahe Li and Paula T. Hammond日期:02/05/2021,浏览量:2353,Q&A: 0
Activating the STING (stimulator of interferon genes) signaling pathway via administration of STING agonist cyclic GMP-AMP (cGAMP) has shown great promise in cancer immunotherapy. While state-of-the-art approaches have predominantly focused on the encapsulation of cGAMP into liposomes or polymersomes for cellular delivery, we discovered that the ...
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神经科学

Relative Quantification of NaV1.1 Protein in Mouse Brains Using a Meso Scale Discovery-Electrochemiluminescence (MSD-ECL) Method 利用中尺度发现电化学发光(MSD-ECL)方法对小鼠脑中NaV1.1蛋白进行相对定量 作者:Zhou Han, Anne Christiansen, Meena and Gene Liau日期:02/05/2021,浏览量:1018,Q&A: 0

Densitometric analysis is often used to quantify NaV1.1 protein on immunoblots, although the sensitivity and dilution linearity of the method are usually poor. Here we present a protocol for quantification of NaV1.1 in mouse brain tissues using a Meso Scale Discovery-Electrochemiluminescence (MSD-ECL) method. MSD-ECL is based on ELISA

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Immuno-electrophysiology on Neuromuscular Junctions of Drosophila Third Instar Larva 果蝇三龄幼虫神经肌肉连接的免疫电生理研究 作者:Raffaella Klima, Giulia Romano, Monsurat Gbadamosi, Aram Megighian and Fabian Feiguin日期:02/05/2021,浏览量:1006,Q&A: 0

Alterations in synaptic transmission are critical early events in neuromuscular disorders. However, reliable methodologies to analyze the functional organization of the neuromuscular synapses are still needed. This manuscript provides a detailed protocol to analyze the molecular assembly of the neuromuscular synapses through

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植物科学

Histochemical Staining of Suberin in Plant Roots 植物根部软木脂的组织化学染色 作者:Peter Marhavý and Shahid Siddique日期:02/05/2021,浏览量:2163,Q&A: 0

Histological stains are useful tools for characterizing cell shape, arrangement and the material they are made from. Stains can be used individually or simultaneously to mark different cell structures or polymers within the same cells, and to visualize them in different colors. Histological stains can be combined with genetically-encoded

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Measuring Extracellular Proton and Anionic Fluxes in Arabidopsis Pollen Tubes 拟南芥花粉管细胞外质子和阴离子通量的测定 作者:Maria Teresa Portes and José A. Feijó日期:02/05/2021,浏览量:2169,Q&A: 0

The ion-selective vibrating probe has been used to detect and quantify the magnitude and direction of transmembrane fluxes of several ions in a wide range of biological systems. Inherently non-invasive, vibrating probes have been essential to access relevant electrophysiological parameters related to apical growth and morphogenesis in pollen

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Plant ARGONAUTE Protein Immunopurification for Pathogen Cross Kingdom Small RNA Analysis 植物ARGONAUTE蛋白免疫纯化用于病原体跨界小RNA分析 作者:Florian Dunker, Bernhard Lederer and Arne Weiberg日期:02/05/2021,浏览量:2127,Q&A: 0

Over the last decade, it has been noticed that microbial pathogens and pests deliver small RNA (sRNA) effectors into their host plants to manipulate plant physiology and immunity for infection, known as cross kingdom RNA interference. In this process, fungal and oomycete parasite sRNAs hijack the plant ARGONAUTE (AGO)/RNA-induced silencing complex

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A Pipeline for Non-model Organisms for de novo Transcriptome Assembly, Annotation, and Gene Ontology Analysis Using Open Tools: Case Study with Scots Pine 使用开放工具进行从头转录组组装、注释和基因本体分析的非模式生物管道:Scots Pine案例研究 作者:Gustavo T. Duarte, Polina Yu. Volkova and Stanislav A. Geras’kin日期:02/05/2021,浏览量:3055,Q&A: 0

RNA sequencing (RNA-seq) has opened up the possibility of studying virtually any organism at the whole transcriptome level. Nevertheless, the absence of a sequenced and accurately annotated reference genome may be an obstacle for applying this technique to non-model organisms, especially for those with a complex genome. While de novo transcriptome

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干细胞

Rapid and Simplified Induction of Neural Stem/Progenitor Cells (NSCs/NPCs) and Neurons from Human Induced Pluripotent Stem Cells (hiPSCs) 从人诱导的多能干细胞(hiPSCs)快速简便地诱导神经干/祖细胞(NSCs/NPCs)和神经元 作者:Ryutaro Kajihara, Tadahiro Numakawa and Takumi Era日期:02/05/2021,浏览量:2117,Q&A: 0

Human induced pluripotent stem cells (iPSCs) and their progeny displaying tissue-specific characteristics have paved the way for regenerative medicine and research in various fields such as the elucidation of the pathological mechanism of diseases and the discovery of drug candidates. iPSC-derived neurons are particularly valuable as it is

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