• 卷册 11, 2021
  • 卷册 10, 2020
  • 卷册 9, 2019
  • 卷册 8, 2018
  • 卷册 7, 2017
  • 卷册 6, 2016
  • 卷册 5, 2015
  • 卷册 4, 2014
  • 卷册 3, 2013
  • 卷册 2, 2012
  • 卷册 1, 2011
Image Credit: BioProtoc.3954

往期刊物(2021)

卷册: 11 期号: 6

生物化学

Monitoring Real-time Temperature Dynamics of a Short RNA Hairpin Using Förster Resonance Energy Transfer and Circular Dichroism 利用荧光共振能量转移和圆二色性监测短发夹RNA的实时温度动态 作者:Martin Balcerowicz, Marco Di Antonio and Betty Y. W. Chung日期:03/20/2021,浏览量:1027,Q&A: 0

RNA secondary structures are highly dynamic and subject to prompt changes in response to the environment. Temperature in particular has a strong impact on RNA structural conformation, and temperature-sensitive RNA hairpin structures have been exploited by multiple organisms to modify the rate of translation in response to temperature changes.

...
更多 >>

生物医学工程

Multilayered Fabrication Assembly Technique to Engineer a Corneal Stromal Equivalent 用多层制造组装技术制做角膜基质等效物 作者:Julia Fernández-Pérez, Peter W. Madden and Mark Ahearne日期:03/20/2021,浏览量:813,Q&A: 0

Tissue engineering has emerged as a strategy to combat the donor shortage of human corneas for transplantation. Synthetic corneal substitutes are currently unable to support the normal phenotype of human cells and so decellularized animal corneas have been deployed to more closely provide the topographical and biochemical cues to promote cell

...
更多 >>

癌症生物学

Intestinal Co-culture System to Study TGR5 Agonism and Gut Restriction 利用肠道共培养系统研究TGR5激动剂和肠道限制性 作者:Snehal N. Chaudhari and A. Sloan Devlin日期:03/20/2021,浏览量:794,Q&A: 0

The activation of the Takeda G-protein receptor 5 (TGR5, also known as the G protein-coupled bile acid receptor 1, GPBAR1) in enteroendocrine L-cells results in secretion of the anti-diabetic hormone Glucagon-Like Peptide 1 (GLP-1) into systemic circulation. Consequently, recent research has focused on identification and development of TGR5

...
更多 >>
Preparation of an Orthotopic, Syngeneic Model of Lung Adenocarcinoma and the Testing of the Antitumor Efficacy of Poly(2-oxazoline) Formulation of Chemo-and Immunotherapeutic Agents 肺腺癌原位同源模型的制备及聚恶唑啉化疗和免疫治疗药物抗肿瘤疗效的测定 作者:Natasha Vinod, Duhyeong Hwang, Salma H. Azam, Amanda E. D. Van Swearingen, Elizabeth Wayne, Sloane Christian Fussell, Marina Sokolsky-Papkov, Chad V. Pecot and Alexander V. Kabanov日期:03/20/2021,浏览量:891,Q&A: 0

Tumor xenograft models developed by transplanting human tissues or cells into immune-deficient mice are widely used to study human cancer response to drug candidates. However, immune-deficient mice are unfit for investigating the effect of immunotherapeutic agents on the host immune response to cancer (Morgan, 2012). Here, we describe the

...
更多 >>
Preparation and Characterization of Poly(2-oxazoline) Micelles for the Solubilization and Delivery of Water Insoluble Drugs 用于溶解和输送难溶性药物的聚恶唑啉胶束的制备及特性 作者:Natasha Vinod, Duhyeong Hwang, Salma H. Azam, Amanda E. D. Van Swearingen, Elizabeth Wayne, Sloane Christian Fussell, Marina Sokolsky-Papkov, Chad V. Pecot and Alexander V. Kabanov日期:03/20/2021,浏览量:909,Q&A: 0

Many new drug development candidates are highly lipophilic compounds with low water solubility. This constitutes a formidable challenge for the use of such compounds for cancer therapy, where high doses and intravenous injections are needed (Di et al., 2012). Here, we present a poly(2-oxazoline) polymer (POx)-based nanoformulation strategy to

...
更多 >>

细胞生物学

Retention Using Selective Hooks (RUSH) Cargo Sorting Assay for Live-cell Vesicle Tracking in the Secretory Pathway Using HeLa Cells 用RUSH蛋白分选法追踪HeLa细胞分泌通路中的活细胞囊泡 作者:Mehrshad Pakdel, Natalia Pacheco-Fernandez and Julia von Blume日期:03/20/2021,浏览量:803,Q&A: 0

More than 30% of the total amount of proteins synthesized in mammalian cells follow the secretory pathway in order to mature and be properly sorted to their final destinations. Among several methodologies that describe live-cell monitoring of vesicles, the Retention Using Selective Hooks (RUSH) system is a powerful one that allows to visualize

...
更多 >>

免疫学

Murine Monocyte and Macrophage Culture 小鼠单核细胞和巨噬细胞培养 作者:Simone M. Haag and Aditya Murthy日期:03/20/2021,浏览量:1334,Q&A: 0

Myeloid progenitors in the bone marrow generate monocytes, macrophages, granulocytes and most dendritic cells. Even though these innate immune cells are part of the same lineage, each cell type plays a specific and critical role in tissue development, host defense and the generation of adaptive immunity. Protocols have been developed in the past

...
更多 >>
Liposomal Clodronate-mediated Macrophage Depletion in the Zebrafish Model 在斑马鱼模型中脂质体氯膦酸盐介导的巨噬细胞耗竭 作者:Linlin Yang, Alison M. Rojas and Celia E. Shiau日期:03/20/2021,浏览量:678,Q&A: 0

The ability to conduct in vivo macrophage-specific depletion remains an effective means to uncover functions of macrophages in a wide range of physiological contexts. Compared to the murine model, zebrafish offer superior imaging capabilities due to their optical transparency starting from a single-cell stage to throughout larval development.

...
更多 >>
Quantitative Measurement of Mucolytic Enzymes in Fecal Samples 粪便粘液溶解酶的定量测定 作者:Shahanshah Khan and Hasan Zaki日期:03/20/2021,浏览量:753,Q&A: 0

The mucus layer in the gastrointestinal tract covers the apical surface of intestinal epithelial cells, protecting the mucosal tissue from enteric pathogen and commensal microorganisms. The mucus is primarily composed of glycosylated protein called mucins, which are produced by goblet cells, a type of columnar epithelial cells in the intestinal

...
更多 >>
An Image-based Dynamic High-throughput Analysis of Adherent Cell Migration 一种基于图像的动态高通量分析黏附细胞的迁移 作者:Meng Sun, Bence Rethi, Akilan Krishnamurthy, Vijay Joshua, Heidi Wähämaa, Sergiu-Bogdan Catrina and Anca Catrina日期:03/20/2021,浏览量:852,Q&A: 0

In this protocol, we describe a method to monitor cell migration by live-cell imaging of adherent cells. Scratching assay is a common method to investigate cell migration or wound healing capacity. However, achieving homogenous scratching, finding the optimal time window for end-point analysis and performing an objective image analysis imply, even

...
更多 >>

微生物学

A Spectrofluorophotometrical Method Based on Fura-2-AM Probe to Determine Cytosolic Ca2+ Level in Pseudomonas syringae Complex Bacterial Cells 基于Fura-2-AM探针的荧光光度法测定丁香假单胞菌复合菌胞内Ca2+水平 作者:Simone Trabalza, Roberto Buonaurio, Alberto M. Del Pino, Carlo A. Palmerini, Harrold A. van den Burg and Chiaraluce Moretti日期:03/20/2021,浏览量:788,Q&A: 0

Calcium signaling is an emerging mechanism by which bacteria respond to environmental cues. To measure the intracellular free-calcium concentration in bacterial cells, [Ca2+]i, a simple spectrofluorometric method based on the chemical probe Fura 2-acetoxy methyl ester (Fura 2-AM) is here presented using Pseudomonad bacterial cells. This is an

...
更多 >>
Detection and Quantification of African Swine Fever Virus in MA-104 Cells 非洲猪瘟病毒在MA-104细胞中的检测与定量 作者:Ayushi Rai, Sarah Pruitt, Elizabeth Ramirez-Medina, Elizabeth A. Vuono, Ediane Silva, Lauro Velazquez-Salinas, Consuelo Carrillo, Manuel V. Borca and Douglas P. Gladue日期:03/20/2021,浏览量:820,Q&A: 0

Detection of live African swine fever virus (ASFV) has historically relied on the use of primary swine macrophages (PSM). PSM do not replicate and have to be isolated fresh from donor swine. We previously identified that a MA-104 cells (ATCC #CRL-2378.1), a commercially available cell line isolated from African green monkey (Cercopithecus

...
更多 >>

分子生物学

Real-time Base Excision Repair Assay to Measure the Activity of the 8-oxoguanine DNA Glycosylase 1 in Isolated Mitochondria of Human Skin Fibroblasts 实时碱基切除修复法测定人皮肤纤维细胞的分离线粒体中8-氧鸟嘌呤DNA糖基化酶1的活性 作者:Daniel Schniertshauer, Daniel Gebhard and Jörg Bergemann日期:03/20/2021,浏览量:530,Q&A: 0

7,8-dihydro-8-oxoguanine (8-oxoG) is one of the most common and mutagenic oxidative DNA damages induced by reactive oxygen species (ROS). Since ROS is mainly produced in the inner membranes of the mitochondria, these organelles and especially the mitochondrial DNA (mtDNA) contained therein are particularly affected by this damage. Insufficient

...
更多 >>
Colorimetric RT-LAMP and LAMP-sequencing for Detecting SARS-CoV-2 RNA in Clinical Samples 比色RT-LAMP和LAMP测序检测临床样本中SARS-CoV-2 RNA 作者:Konrad Herbst, Matthias Meurer, Daniel Kirrmaier, Simon Anders, Michael Knop and Viet Loan Dao Thi日期:03/20/2021,浏览量:777,Q&A: 0

During pandemics, such as the one caused by SARS-CoV-2 coronavirus, simple methods to rapidly test large numbers of people are needed. As a faster and less resource-demanding alternative to detect viral RNA by conventional qPCR, we used reverse transcription loop-mediated isothermal amplification (RT-LAMP). We previously established colorimetric

...
更多 >>

神经科学

Ligand and Carbohydrate Engagement (LACE) Assay and Fluorescence Quantification on Murine Neural Tissue 小鼠神经组织中配体和碳水化合物结合(LACE)实验和荧光定量测定 作者:James M. Clegg and Thomas Pratt日期:03/20/2021,浏览量:425,Q&A: 0

The interaction between cell surface heparan sulphate and diffusible ligands such as FGFs is of vital importance for downstream signaling, however, there are few techniques that can be used to investigate this binding event. The ligand and carbohydrate engagement (LACE) assay is a powerful tool which can be used to probe the molecular interaction

...
更多 >>
Investigate Synaptic Vesicles Mobility in Neuronal Culture Axons by FRAP Imaging 用FRAP成像技术研究神经元轴突中突触囊泡的移动 作者:Xiao Min Zhang, Fabrice P. Cordelieres and Etienne Herzog日期:03/20/2021,浏览量:603,Q&A: 0

Synaptic vesicles (SVs) are clustered in the presynaptic terminals and consistently trafficking along axons. Based on their release features, SVs are classified into different “pools”. Imaging of SVs that are traveling among multiple presynaptic terminals has helped define a new pool named “SV super-pool”. Here we describe

...
更多 >>
A Time Duration Discrimination Task for the Study of Elapsed Time Processing in Rats 用于研究大鼠执行时间的时程辨别任务 作者:Sarah Tenney, Eleftheria Vogiatzoglou, Deena Chohan, Annette Vo, Thomas Hunt, Kayla Cayanan, Jena B. Hales and Marta Sabariego日期:03/20/2021,浏览量:673,Q&A: 0

Space and time are both essential features of episodic memory. However, while spatial tasks have been used effectively to study the behavioral relevance of place cells, the behavioral paradigms utilized for the study of time cells have not used time duration as a variable that animals need to be aware of to solve the task. In order to evaluate how

...
更多 >>