• 卷册 12, 2022
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Image Credit: BioProtoc.4192

生物化学

Co-immunofluorescence of MRPL12 and Nrf2 in HK2 Cells MRPL12和Nrf2在HK2细胞中的联合免疫荧光研究 作者:Yao Lu, Xia Gu, Qiang Wan, Hong Feng and Yi Liu日期:10/20/2021,浏览量:707,Q&A: 0

Immunofluorescence is a technique to visualize the localization of specific molecule targets within cells using the specificity of antibodies. Here, we describe a protocol to detect two different protein components in a cell simultaneously. Antibody concentrations to be used vary from cell to cell and should be optimized for different cell types.

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生物物理学

Measurement of the Translational Diffusion Coefficient and Hydrodynamic Radius of Proteins by Dynamic Light Scattering 动态光散射法测定蛋白质的平移扩散系数和流体动力学半径 作者:Ernest T. Parker and Pete Lollar日期:10/20/2021,浏览量:979,Q&A: 0

Diffusion is a fundamental process in biological systems that governs the molecular collisions driving biochemical reactions and membrane and transport. Measurement of the diffusion coefficient and application of the Stokes-Einstein equation produces the hydrodynamic radius, which is a commonly used gauge of particle size. Additionally,

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Electrophysiological Recordings of the Polycystin Complex in the Primary Cilium of Cultured Mouse IMCD-3 Cell Line 小鼠IMCD-3细胞系原代纤毛中多囊素复合物的电生理记录 作者:Kotdaji Ha and Markus Delling日期:10/20/2021,浏览量:586,Q&A: 0

PC-1 and PC-2 form an ion channel complex called the polycystin complex, which predominantly localizes to a small hair-like organelle called the primary cilium. The polycystin complex permeates cations, K+, Na+, and Ca2+, and has an unusual 1:3 stoichiometry that combines one PC-1 subunit with three PC-2 subunits. However, the small size and shape

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Biophysical Characterization of Iron-Sulfur Proteins 铁硫蛋白的生物物理特性 作者:Bhanu P. Jagilinki, Irina Paluy, Alexei M. Tyryshkin, Vikas Nanda and Dror Noy日期:10/20/2021,浏览量:670,Q&A: 0

Iron-sulfur proteins are primordial catalysts and biological electron carriers that today drive major metabolic pathways across all forms of life. They can access a diversity of oxidation states and can mediate electron transfer over an extended range of reduction potentials spanning more than 1 V. Depending on the protein micro-environment and

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癌症生物学

Urea Denaturation, Zinc Binding, and DNA Binding Assays of Mutant p53 DNA-binding Domains and Full-length Proteins 突变p53 DNA结合域和全长蛋白的尿素变性、锌结合和DNA结合分析 作者:Jeung-Hoi Ha, Xin Yu, Darren R. Carpizo and Stewart N. Loh日期:10/20/2021,浏览量:542,Q&A: 0

In the cell, the thermodynamic stability of a protein – and hence its biological activity – can change dramatically as a result of perturbations in its amino acid sequence and the concentration of stabilizing ligands. This interplay is particularly evident in zinc-binding transcription factors such as the p53 tumor suppressor, whose

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细胞生物学

Collection of in vivo Capacitated Sperm from Different Locations Along the Reproductive Tract of Time-Mated Female Mice by Microdissection 定时交配的雌性小鼠生殖道不同位置的体内充能精子的显微解剖收集 作者:Lukas Ded and Jean-Ju Chung日期:10/20/2021,浏览量:1019,Q&A: 0

Mammalian sperm cells are not capable of fertilizing an egg immediately after ejaculation; instead, they must gradually acquire the capacity to fertilize while they travel inside the female reproductive tract. Sperm cells are transported by the muscular activity of the myometrium to the utero-tubal junction (UTJ) before entering the oviduct where

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发育生物学

Whole-mount Immunohistochemistry to Visualize Mouse Embryonic Dermal Lymphatic Vasculature 小鼠胚胎真皮淋巴血管的免疫组织化学观察 作者:Xin Geng and R. Sathish Srinivasan日期:10/20/2021,浏览量:583,Q&A: 0

Lymphatic vessels are abundant in the skin where they regulate interstitial fluid uptake and immune surveillance. Defects in dermal lymphatic vessels, such as fewer vessels and abnormal lymphatic vessel coverage with mural cells, are frequently associated with lymphedema and other lymphatic disorders. Whole-mount immunohistochemistry allows the

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Isolation and Culturing Primary Cholangiocytes from Mouse Liver 小鼠肝原代胆管细胞的分离与培养 作者:Ramesh Kudira, Bal Krishan Sharma, Mary Mullen, Sujit K. Mohanty, Bryan Donnelly, Gregory M. Tiao and Alexander Miethke日期:10/20/2021,浏览量:737,Q&A: 0

Cholangiocytes are epithelial cells lining the intrahepatic and extrahepatic bile ducts. Cholangiocytes perform key physiological functions in the liver. Bile synthesized by hepatocytes is secreted into bile canaliculi, further stored in the gallbladder, and finally discharged into the duodenum. Due to liver injury, biliary epithelial proliferate

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微生物学

A Rapid Induction Overexpression System for the Fission Yeast Schizosaccharomyces pombe 裂解酵母裂殖酵母的快速诱导过表达系统 作者:Angad Garg日期:10/20/2021,浏览量:453,Q&A: 0

The fission yeast Schizosaccharomyces pombe is an excellent genetically tractable model organism used in the study of conserved eukaryotic cellular biology. One genetic tool in the assessment of gene function is the in vivo overexpression of proteins. Existing overexpression tools have limitations of induction kinetics, dynamic range, and/or

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Quantification of RuBisCO Expression and Photosynthetic Oxygen Evolution in Cyanobacteria 蓝藻RuBisCO表达和光合氧释放的定量研究 作者:Mateusz Kędzior and Betul Kacar日期:10/20/2021,浏览量:611,Q&A: 0

Phototrophic microorganisms are frequently engineered to regulate the expression and the activity of targeted enzymes of interest for specific biotechnological and agricultural applications. This protocol describes a method to evaluate the expression of RuBisCO (ribulose 1,5-bisphosphate carboxylase/oxygenase) in the model cyanobacterium

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神经科学

Simultaneous Monitoring Cytoplasmic Calcium Ion and Cell Surface Phosphatidylserine in the Necrotic Touch Neurons of Caenorhabditis elegans 秀丽隐杆线虫坏死触觉神经元胞质钙离子和细胞表面磷脂酰丝氨酸的同时监测 作者:Yoshitaka Furuta and Zheng Zhou日期:10/20/2021,浏览量:493,Q&A: 0

Calcium ions trigger many cellular events, including the release of neurotransmitters at the synaptic terminal and excitotoxic cell death. Recently, we have discovered that a transient increase in the level of cytoplasmic Ca2+ triggers the exposure of phosphatidylserine (PS) on the surfaces of necrotic cells in the nematode Caenorhabditis elegans.

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Isolation and Electrophysiology of Murine Sympathetic Postganglionic Neurons in the Thoracic Paravertebral Ganglia 小鼠胸椎旁神经节交感神经节后神经元的分离与电生理研究 作者:Mallika Halder, Michael Lee McKinnon, Yaqing Li, Peter Wenner and Shawn Hochman日期:10/20/2021,浏览量:572,Q&A: 0

The thoracic paravertebral sympathetic chain postganglionic neurons (tSPNs) represent the predominant sympathetic control of vascular function in the trunk and upper extremities. tSPNs cluster to form ganglia linked by an interganglionic nerve and receive multisegmental convergent and divergent synaptic input from cholinergic sympathetic

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Isolation and Phospholipid Enrichment of Muscle Mitochondria and Mitoplasts 肌肉线粒体和有丝分裂体的分离和磷脂富集 作者:Alexandre Prola, Aymeline Vandestienne, Nabil Baroudi, Frederic Joubert, Laurent Tiret and Fanny Pilot-Storck日期:10/20/2021,浏览量:531,Q&A: 0

The efficient ATP production in mitochondria relies on the highly specific organization of its double membrane. Notably, the inner mitochondrial membrane (IMM) displays a massive surface extension through its folding into cristae, along which concentrate respiratory complexes and oligomers of the ATP synthase. Evidence has accumulated to highlight

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植物科学

High Throughput Analyses of Ascorbate-turnover Enzyme Activities in Rice (Oryza sativa L.) Seedlings 水稻幼苗抗坏血酸转化酶活性的高通量分析 作者:Lin-Bo Wu, Yanru Feng, Frederike Zeibig, Muhammad Shahedul Alam and Michael Frei日期:10/20/2021,浏览量:722,Q&A: 0

Ascorbate (Vitamin C) fulfills various functions in plant photosynthesis and abiotic stress tolerance. The four key enzymes involved in the ascorbate-turnover pathway are ascorbate peroxidase, ascorbate oxidase, monodehydroascorbate reductase, and dehydroascorbate reductase. Several reports have shown the pivotal roles of these enzymes in plant

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Proteoliposomes for Studying Lipid-protein Interactions in Membranes in vitro 体外研究膜脂-蛋白相互作用的蛋白质脂质体 作者:Helmut Kirchhoff日期:10/20/2021,浏览量:576,Q&A: 0

Lipids in biomembranes can control the structure and, therefore, the functionality of membrane-embedded protein complexes. Unraveling how the lipid composition determines the mode of operation of membrane proteins provides mechanistic insights into their functionality. We applied a proteoliposome technique for studying how proteins function in

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High-throughput Screening for Defense Priming-inducing Compounds in Parsley Cell Cultures 欧芹细胞防御引物诱导化合物的高通量筛选 作者:Kathrin Schmitz, Linda Werner and Uwe Conrath日期:10/20/2021,浏览量:553,Q&A: 0

Defense priming describes the enhanced potency of cells to activate defense responses. Priming accompanies local and systemic immune responses and can be triggered by microbial infection or upon treatment with certain chemicals. Thus, chemically activating defense priming is promising for biomedicine and agriculture. However, test systems for

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