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生物化学

Assay to Access Anthelmintic Activity of Small Molecule Drugs Using Caenohabidtis elegans as a Model 使用秀丽隐杆线虫作为模型测定小分子药物的驱肠虫活性 作者:Viviane Sant’Anna, Wanderley de Souza and Rossiane C. Vommaro日期:01/20/2017,浏览量:8854,Q&A: 0
This protocol proposes to use the nematode Caenorhabditis elegans as a model to screen and study the anthelmintic activity of natural and synthetic compounds and to observe their effects on the morphology and the ultrastructure of the helminths. Furthermore, C. elegans can be used to investigate the anthelmintic activity in ...
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癌症生物学

In vitro Dephosphorylation Assay of c-Myc c-Myc体外去磷酸化实验 作者:Peng Liao, Weichao Wang and Xin Ge日期:01/20/2017,浏览量:6689,Q&A: 0
This protocol describes experimental procedures for in vitro dephosphorylation assay of human protein c-Myc. This protocol can be adapted to detect phosphatase activity of other Ser/Thr phosphatases.
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Assessment of Cellular Redox State Using NAD(P)H Fluorescence Intensity and Lifetime 利用NAD(P)H荧光强度和寿命评估细胞氧化还原状态 作者:Thomas S. Blacker, Tunde Berecz, Michael R. Duchen and Gyorgy Szabadkai日期:01/20/2017,浏览量:10319,Q&A: 0
NADH and NADPH are redox cofactors, primarily involved in catabolic and anabolic metabolic processes respectively. In addition, NADPH plays an important role in cellular antioxidant defence. In live cells and tissues, the intensity of their spectrally-identical autofluorescence, termed NAD(P)H, can be used to probe the mitochondrial redox state, ...
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发育生物学

P-body and Stress Granule Quantification in Caenorhabditis elegans 秀丽隐杆线虫中P-body和应激颗粒的定量 作者:Matthias Rieckher and Nektarios Tavernarakis日期:01/20/2017,浏览量:10377,Q&A: 0
Eukaryotic cells contain various types of cytoplasmic, non-membrane bound ribonucleoprotein (RNP) granules that consist of non-translating mRNAs and a versatile set of associated proteins. One prominent type of RNP granules is Processing bodies (P bodies), which majorly harbors translationally inactive mRNAs and an array of proteins mediating mRNA ...
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Transplantation of Mesenchymal Cells Including the Blastema in Regenerating Zebrafish Fin 再生斑马鱼鳍中间充质细胞(包括芽基)的移植 作者:Eri Shibata, Kazunori Ando and Atsushi Kawakami日期:01/20/2017,浏览量:7482,Q&A: 0
Regeneration of fish fins and urodele limbs occurs via formation of the blastema, which is a mass of mesenchymal cells formed at the amputated site and is essential for regeneration. The blastema transplantation, a novel technique developed in our previous studies (Shibata et al., 2016; Yoshinari et al., 2012) is a useful ...
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免疫学

Isolation of PBMCs Using Vacutainer® Cellular Preparation Tubes (CPTTM) 使用Vacutainer®细胞制备管(CPTTM)分离外周血单核细胞 作者:Alaina Puleo, Chantia Carroll, Holden Maecker and Rohit Gupta日期:01/20/2017,浏览量:31447,Q&A: 0
Peripheral blood mononuclear cell (PBMC) isolation is commonly done via density gradient centrifugation over Ficoll-Hypaque, a labor-intensive procedure that requires skilled technicians and can contribute to sample variability. Cellular Preparation Tubes (CPTs) are Vacutainer blood draw tubes that contain Ficoll-Hypaque and a gel plug that ...
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Virus Binding and Internalization Assay for Adeno-associated Virus 腺相关病毒的病毒结合和内化测定实验 作者:Garrett E. Berry and Longping V. Tse日期:01/20/2017,浏览量:9659,Q&A: 0
The binding and internalization of adeno-associated virus (AAV) is an important determinant of viral infectivity and tropism. The ability to dissect these two tightly connected cellular processes would allow better understanding and provide insight on virus entry and trafficking. In the following protocol, we describe a quantitative PCR (qPCR) ...
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微生物学

Aggregation Prevention Assay for Chaperone Activity of Proteins Using Spectroflurometry 使用荧光光谱法测定蛋白质聚集抑制实验中分子伴侣的活性 作者:Manish Bhuwan, Madhuri Suragani, Nasreen Z. Ehtesham and Seyed E. Hasnain日期:01/20/2017,浏览量:9771,Q&A: 0
The ability to stabilize other proteins against thermal aggregation is one of the major characteristics of chaperone proteins. Molecular chaperones bind to nonnative conformations of proteins. Folding of the substrate is triggered by a dynamic association and dissociation cycles which keep the substrate protein on track of the folding pathway ...
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Protein Expression Protocol for an Adenylate Cyclase Anchored by a Vibrio Quorum Sensing Receptor 由弧菌群体感应受体锚定的腺苷酸环化酶膜的催化结构域的蛋白表达方案 作者:Stephanie Beltz and Joachim E. Schultz日期:01/20/2017,浏览量:7007,Q&A: 0
The direct regulation of a mycobacterial adenylate cyclase (Rv1625c) via exchange of its membrane anchor by the quorum sensing receptor CqsS (Vibrio harveyi) has recently been reported (Beltz et al., 2016). This protocol describes the expression and membrane preparation for these chimeric proteins.
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Quantification of Triphenyl-2H-tetrazoliumchloride Reduction Activity in Bacterial Cells 利用四唑红定量分析细菌细胞内的还原活性 作者:Roberto Defez, Anna Andreozzi and Carmen Bianco日期:01/20/2017,浏览量:8201,Q&A: 0
This protocol describes the use of the 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) salt to evaluate the cell redox potential of rhizobia cells. The production of brightly colored and insoluble 1,3,5-Triphenyltetrazolium formazan arising from TTC reduction is irreversible and can be easily quantified using a spectrophotometer. This protocol ...
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神经科学

An Acute Mouse Spinal Cord Slice Preparation for Studying Glial Activation ex vivo 用于研究胶质细胞离体激活的急性小鼠脊髓切片的制备 作者:Juan Mauricio Garré, Guang Yang, Feliksas F. Bukauskas and Michael V. L. Bennett日期:01/20/2017,浏览量:8664,Q&A: 0
Pathological conditions such as amyotrophic lateral sclerosis, spinal cord injury and chronic pain are characterized by activation of astrocytes and microglia in spinal cord and have been modeled in rodents. In vivo imaging at cellular level in these animal models is limited due to the spinal cord’s highly myelinated funiculi. The ...
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植物科学

Nitrate Assay for Plant Tissues 植物组织中的硝酸盐含量测定 作者:Lufei Zhao and Yong Wang日期:01/20/2017,浏览量:14185,Q&A: 4
Nitrogen is an essential macronutrient for plant growth and nitrate content in plants can reflect the nitrogen supply of soil. Here, we provide the salicylic acid method to evaluate the nitrate content in plant tissues. The method is reliable and stable, thus it can be a good choice for measurement of nitrate in plant tissues.
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In vitro Floral Induction of Cuscuta reflexa 大花菟丝子的体外成花诱导 作者:Priyanka Das and Santilata Sahoo日期:01/20/2017,浏览量:6322,Q&A: 0
Floral initiation and development in the angiosperms is the essential step on which the yield of the plant depends. Sometimes external climate or any abiotic stress hinders the floral initiation and ultimately affect the plant yield. Hence, in vitro floral induction and development can help to overcome the external climatic factor. ...
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Pseudomonas syringae Flood-inoculation Method in Arabidopsis 拟南芥的丁香假单胞菌浸没接种法 作者:Yasuhiro Ishiga, Takako Ishiga, Yuki Ichinose and Kirankumar S. Mysore日期:01/20/2017,浏览量:9534,Q&A: 0
Pseudomonas syringae pv. tomato strain DC3000 (Pto DC3000), which causes bacterial speck disease of tomato, has been used as a model pathogen because of its pathogenicity on Arabidopsis thaliana. Here, we demonstrate a rapid and reliable flood-inoculation method based on young Arabidopsis seedlings ...
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Bioassay of Xanthomonas albilineans Attachment on Sugarcane Leaves 采用生物测定法研究甘蔗叶白纹黄单胞菌的附着 作者:Imène Mensi, Jean-Heinrich Daugrois and Philippe Rott日期:01/20/2017,浏览量:7480,Q&A: 0
Sugarcane (interspecific hybrids of Saccharum species) is an economically important crop that provides 70% of raw table sugar production worldwide and contributes, in some countries, to bioethanol and electricity production. Leaf scald, caused by the bacterial plant pathogen Xanthomonas albilineans, is one of the major diseases ...
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Two-electrode Voltage-clamp Recordings in Xenopus laevis Oocytes:Reconstitution of Abscisic Acid Activation of SLAC1 Anion Channel via PYL9 ABA Receptor 非洲爪蟾卵母细胞中的双电极电压钳记录技术:脱落酸通过ABA受体PYL9激活离子通道SLAC1过程的重建 作者:Cun Wang, Jingbo Zhang and Julian I. Schroeder日期:01/20/2017,浏览量:9258,Q&A: 0
Two-Electrode Voltage-Clamp (TEVC) recording in Xenopus laevis oocytes provides a powerful method to investigate the functions and regulation of ion channel proteins. This approach provides a well-known tool to characterize ion channels or transporters expressed in Xenopus laevis oocytes. The plasma membrane of the oocyte is ...
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