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发育生物学

Tandem Affinity Purification in Drosophila Heads and Ovaries 在果蝇头部和卵巢中进行串联亲和纯化 作者:Anita Pepper, Balpreet Bhogal and Thomas Jongens日期:08/05/2012,浏览量:12348,Q&A: 0
Tandem affinity purification (TAP) (Pugi et al.,2001; Rigaut et al., 1999) is a method that uses a tagging approach of a target protein of interest for a two-step purification scheme in order to pull down protein complexes under native conditions and expression levels. The TAP tag consists of three components: a ...
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微生物学

Construction and Screening of a Transposon Insertion Library of Yersinia enterocolitica (YeO3-R1) 小肠结肠炎耶尔森菌 (YeO3-R1)转座子插入库的建立和筛选 作者:Maria Pajunen, Elise Pinta and Mikael Skurnik日期:08/05/2012,浏览量:13094,Q&A: 0
The Mu-transposon system is one of the best characterized transposition systems. Under minimal in vitro set-up, Mu transposition requires only a simple reaction buffer, MuA transposase protein, mini-Mu transposon DNA (donor) and target DNA. The reaction proceeds via initial assembly of the transposition complex that directs transposon ...
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分子生物学

5-Hydroxymethylcytosine (5-hmC) Specific Enrichment 5-羟甲基胞嘧啶 (5-hmC)特异性富集 作者:Keith E Szulwach, Chun-Xiao Song, Chuan He and Peng Jin日期:08/05/2012,浏览量:9086,Q&A: 0
5-Hydroxymethylcytosine (5-hmC) is a newly discovered DNA modification in mammalian genomes. This protocol is to be a highly efficient and selective chemical approach to label and capture 5-hmC, taking advantage of a bacteriophage enzyme that adds a glucose moiety to 5-hmC specifically, which could in turn be used for high-throughput mapping via ...
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Stable Interference by shRNA with pSUPER.retro Vectors and Lipofectamine shRNA 以及逆转录载体pSUPER和脂质体介导的稳定干扰 作者:Silvia Soddu日期:08/05/2012,浏览量:12350,Q&A: 0
RNA interference is a powerful genetic approach for efficiently silencing target genes. Expression of short hairpin RNAs (shRNAs) allows analysis of the consequences of stably silencing genes. This protocol describes a method to stably integrate shRNA constructs with pSUPER.retro vectors and Lipofectamine in RKO and H1299 cells. This method can be ...
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A Quick, No Frills Approach to Mouse Genotyping 小鼠基因型快捷分析法 作者:Manuel E. Lopez日期:08/05/2012,浏览量:23711,Q&A: 1
Mice are extremely powerful mammalian genetic model organisms for basic and medical research, but managing a colony of transgenic mice is time consuming and expensive, many times requiring the help of dedicated technicians. Slow and laborious genotyping procedures add to the hassle. Outsourcing is costly and may not be as fast as desired, ...
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