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细胞生物学

Micrococcal Nuclease (MNase) Assay of Arabidopsis thaliana Nuclei 微球菌核酸酶(Mnase)试验分析拟南芥染色质结构 作者:Laia Armengot and Jordi Moreno-Romero日期:04/05/2013,浏览量:20748,Q&A: 3
Micrococcal nuclease (MNase) is able to produce double-strand breaks within nucleosome linker regions. The efficiency of MNase digestion depends on the degree of chromatin compaction, being more easily digested the regions of less compacted chromatin. The MNase protocol described here can be used to asses changes in the chromatin structure of ...
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免疫学

Allergen Sensitization and Challenge to Ovalbumin 利用卵清蛋白刺激构建致敏模型 作者:François Daubeuf, Laurent Reber and Nelly Frossard日期:04/05/2013,浏览量:15593,Q&A: 0
This protocol describes the sensitization and challenge of mice with ovalbumin for use as an acute murine model of asthma. This protocol induces reproducible airway inflammation and remodelling, and bronchial hyperresponsiveness to methacholine as measured by barometric plethysmography, as well as by the Flexivent® technique in ...
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Measurement of Airway Responsiveness in the Anesthetized Mouse 测定麻醉后小鼠的气道反应性 作者:François Daubeuf, Laurent Reber and Nelly Frossard日期:04/05/2013,浏览量:11093,Q&A: 0
Airway hyperresponsiveness to methacholine is an important characteristic of asthma. Many devices can be used to measure airway responsiveness in the mouse but it is well established that the invasiveness of a measurement technique is correlated with its precision and reproducibility. This protocol describes how to measure airway responses to ...
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微生物学

Neutral Red Assay for Murine Norovirus Replication and Detection in a Mouse 采用中性红试验法进行小鼠诺如病毒增殖和检测 作者:Mariam Bernadette González-Hernández, Jeffrey William Perry and Christiane E. Wobus日期:04/05/2013,浏览量:13402,Q&A: 0
Neutral red (NR) is a dye that must be actively imported into the cell, and, therefore, the dye has been used for decades to selectively stain living cells. In addition, NR can also be incorporated into virus particles, although the mechanism behind this is poorly understood. Once encapsulated into the virion, NR, a light sensitive dye, can be ...
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HIV-1 Single Cycle Infection HIV-1单循环感染 作者:Ferdinand Roesch and Olivier Schwartz日期:04/05/2013,浏览量:10925,Q&A: 0
The role of a viral or cellular protein on HIV-1 infection can sometimes be difficult to assess in a system where the virus is able to replicate for several cycles. Indeed, some effects are only observable at early time points, and can be masked (or on the contrary, artificially increased) after several rounds of viral replication. Therefore, to ...
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Quantification of HIV-1 DNA HIV-1 DNA的量化 作者:Ferdinand Roesch and Olivier Schwartz日期:04/05/2013,浏览量:10364,Q&A: 0
The reverse transcription (RT) reaction is a critical step in HIV-1 life cycle. It is very strongly regulated and the target of several restriction factors (TRIM5α, APOBECs, SAMHD1, etc.). The progress of reverse transcription can be followed by measuring viral DNA by quantitative PCR (qPCR). This method is sensitive enough to allow detection of ...
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Fusarium Virulence Assay on Wheat and Barley Seedlings 小麦和大麦幼苗的抗镰刀菌毒性试验 作者:Lorenzo Covarelli, Donald Gardiner, Giovanni Beccari and Paul Nicholson日期:04/05/2013,浏览量:11778,Q&A: 0
Fusarium root and crown rot is a very important complex disease of small grain cereals worldwide which may lead to very high yield losses. Traditional virulence assays are time consuming and often require plants to be grown in greenhouses or climatic chambers in soil. We describe a rapid laboratory assay for assessing such a disease in wheat and ...
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分子生物学

Western Analysis of Histone Modifications (Aspergillus nidulans) Western检测组蛋白(巢状曲霉)的修饰 作者:Alexandra Soukup and Nancy P. Keller日期:04/05/2013,浏览量:10584,Q&A: 0
Western blotting allows for the specific detection of proteins and/or modifications of proteins by an antibody of interest. This protocol utilizes a crude nuclei extraction protocol for Aspergillus nidulans to enrich for histones and other nuclear proteins prior to gel electrophoresis. Post translational modifications of histones may ...
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Northern Blot of tRNA in Yeast 酵母tRNA的Northern印记杂交实验 作者:Yuehua Wei日期:04/05/2013,浏览量:13010,Q&A: 0
tRNAs are small RNAs around 70-90 nt. tRNAs are different from many other small RNAs in that they are very abundant, which makes it difficult to study their transcriptional regulation by traditional northern blot. Traditional Northern blot involves incorporation of radioactive nucleotides through polymerization, however, tRNA is too short for ...
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Metabolic Labeling of Yeast RNA with Radioactive Uracil 同位素示踪法进行酵母RNA的代谢标记 作者:Yuehua Wei日期:04/05/2013,浏览量:9048,Q&A: 0
To examine gene expression, Northern blot or Real-Time PCR can be used to detect low abundant RNA such as mRNA. However, for high abundant RNAs such as rRNA and tRNA, Northern blot will not be able to discriminate the newly synthesized RNA from total RNA. Therefore, metabolic labeling is necessary to evaluate the expression of rRNA and tRNA genes. ...
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神经科学

Fear Conditioning Assay in Mouse 小鼠恐惧条件反射试验 作者:Melissa Wang and Isabel A. Muzzio日期:04/05/2013,浏览量:12447,Q&A: 0
The study of fear memory is important for understanding various anxiety disorders in which patients experience persistent recollections of traumatic events. These memories often involve associations of contextual cues with aversive events; consequently, Pavlovian classical conditioning is commonly used to study contextual fear learning. A form of ...
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植物科学

Northwestern Blot of Protein-RNA Interaction from Young Rice Panicles RNA-蛋白质印记法研究水稻幼穗中蛋白质-RNA的相互作用 作者:Saminathan Thangasamy and Guang-Yuh Jauh日期:04/05/2013,浏览量:13686,Q&A: 0
The northwestern assay is employed to study the interaction between protein and RNA. The RNA binding proteins tend to bind to different kinds of RNA through either known domains or unknown sequences of proteins. Rice LGD1 recombinant protein, a grass-specific novel protein with RNA binding sequences in its C-terminal, was used to probe its ...
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