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生物化学

In vitro Lipid Transfer Assay 体外脂质转移试验 作者:Yan Zhang and Xiaochen Wang日期:05/05/2013,浏览量:10573,Q&A: 0
This is a protocol to detect lipid transfer activity of NRF-5, a member of the LPS binding/lipid transfer protein family. The lipid transfer activity is examined by using isotope-labeled cholesterol and liposomes, and tested in two directions (Figure 1): from proteins to liposomes and from liposomes to proteins.
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In vitro Lipid Binding Assay 体外脂质结合试验 作者:Yan Zhang and Xiaochen Wang日期:05/05/2013,浏览量:13245,Q&A: 0
This is a protocol to examine in vitro protein-lipid binding using membrane strips coated with various lipids. It has been successfully used to study in vitro interaction between lipids and C. elegans proteins.
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GTP Binding Assays in Arabidopsis thaliana 拟南芥的GTP结合试验 作者:Ana Romina Fox and Maria Agustina Mazzella日期:05/05/2013,浏览量:8651,Q&A: 0
Signaling through G proteins constitutes an ancient mechanism that functions in the transduction of extracellular signals into intracellular responses. Activation of a proper receptor by a stimuli leads to an exchange of GDP for GTP, the activation of G proteins and the dissociation of Gα-GTP from the Gβγ dimer for the ...
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癌症生物学

Subcellular Fractionation of Cultured Human Cell Lines 人源细胞系的亚细胞分离 作者:Zhuoyou Yu, Zhiguang Huang and Maria Li Lung日期:05/05/2013,浏览量:43162,Q&A: 6
Subcellular localization is crucial for the proper functioning of a protein. Deregulation of subcellular localization may lead to pathological consequences and result in diseases like cancer. Immuno-fluorescent staining and subcellular fractionation can be used to determine localization of a protein. Here we discuss a protocol to separate the ...
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发育生物学

Quantification of Cell Corpses, Cell Death Occurrence, Cell Corpse Duration 死细胞、细胞死亡概率以及死细胞持续时间的量化分析 作者:Yan Zhang, Haibin Wang and Xiaochen Wang日期:05/05/2013,浏览量:9831,Q&A: 1
During the development of the C. elegans hermaphrodite, 131 of the 1090 somatic cells generated undergo programmed cell death, among which 113 die during embryogenesis starting from 200-cell stage. The apoptotic cells (also called “cell corpses”) appear as highly refractile button-like objects and are easily identified using differential ...
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免疫学

Cell Isolation of Spleen Mononuclear Cells 脾脏单核细胞的分离 作者:Benno Weigmann日期:05/05/2013,浏览量:29610,Q&A: 0
This method allows you to isolate different subclass mononuclear cells, like B-cells, T cells, CD4+ and CD8+ T, from mouse spleen. By conjugating cells with specific antibodies and subsequently magnetic beads isolation, using the technique from Miltenyi, this allows a high purity.
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植物科学

Modified Single-Cell Transient Gene Expression Assay in Barley Epidermal Cells 大麦表皮细胞中一种改良的单细胞转基因技术-粒子枪法 作者:Shiwei Bai, Cheng Chang, Xinyun Han and Qian-Hua Shen日期:05/05/2013,浏览量:8554,Q&A: 0
Transient gene expression via biolistic particle delivery is a widely used technique for gene functional analysis in plants. In this protocol we describe a modified single-cell transient expression assay through transformation with a particle inflow gun of the model PDS-1000/He system (Bio-Rad). This assay was originally optimized for analyzing ...
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Measuring Stomatal Density in Rice 水稻叶片气孔密度的测定 作者:Kensuke Kusumi日期:05/05/2013,浏览量:16679,Q&A: 1
The number of stomata on leaves is known to be affected by various environmental factors and intrinsic developmental program. Stomatal density and stomatal index are generally used as indicators of the leaf development and the plant growth. This protocol describes an easy, non-destructive method for preparing imprints of the rice leaf surface ...
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干细胞

Single-cell Gene Expression Profiling of Mouse Stem Cells With Fluidigm BiomarkTM Dynamic Array 采用Fluidigm Biomark™动态芯片检测小鼠干细胞中的单细胞基因表达谱 作者:Ana Sevilla日期:05/05/2013,浏览量:16638,Q&A: 3
This protocol describes how to use Fluidigm BiomarkTM 96.96 dynamic arrays for high-throughput expression profiling from single mouse stem cells, assaying up to 96 independent samples with up to 96 quantitative PCR (qPCR) probes (equivalent to 9,216 reactions) in a single experiment. This Dynamic Array contains a network of microfluidic ...
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