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生物化学

Paper Chromatography as Exemplified by Separation of Urocanic Acid and Deaminohistidine 分离尿刊酸和多聚组氨酸的经典方法:纸色谱分析法 作者:Alexander V. Bogachev日期:06/20/2013,浏览量:9818,Q&A: 0
Paper chromatography is an ancient technique to separate low molecular mass compounds based on their distribution between mobile phase (solvent) and stationary phase (cellulose and cellulose-bound water). Paper chromatography has been largely replaced by thin layer chromatography and high performance liquid chromatography as the latter methods ...
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细胞生物学

Calcium Mobilization Assay to Measure the Activity of Gq-coupled Receptors 采用钙流检测法测定Gq偶联受体的活性 作者:Hamiyet Unal日期:06/20/2013,浏览量:21992,Q&A: 0
Calcium mobilization assay is a cell-based second messenger assay to measure the calcium flux associated with Gq-protein coupled receptor activation or inhibition. The method utilizes a calcium sensitive fluorescent dye that is taken up into the cytoplasm of most cells. In some cell lines in which organic-anion transporters are particularly active ...
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LC3B Labeling on Terrestrial Isopod Adipocytes LC3B标记陆生等足目脂肪细胞 作者:Christine Braquart-Varnier, Maryline Raimond and Mathieu Sicard日期:06/20/2013,浏览量:9152,Q&A: 0
The LC3B protein plays a critical role in autophagy. Normally, this protein resides in the cytosol, but following cleavage and lipidation with phosphatidylethanolamine, LC3B associates with the phagophore. This localization can be used as a general marker for autophagic membranes. To visualize the LC3B, we used the LC3B Antibody Kit for Autophagy ...
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发育生物学

Labeling of Precursor Granule Cells in the Cerebellum by ex vivo Electroporation 通过体外电穿孔转染对小脑颗粒细胞前体进行标记 作者:Aya Ito-Ishida日期:06/20/2013,浏览量:13449,Q&A: 1
This protocol will be useful to introduce the genes of interest into the cerebellar granule cells at early stages of development. Since the granule cell precursors are localized in the external granule layer before migration, DNA plasmids can be specifically incorporated into the granule cells by injecting DNA solution into the cerebellar fissures ...
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微生物学

Amino Acid Transport Assays in Resting Cells of Lactococcus lactis 乳酸乳球菌静息细胞中的氨基酸转运分析 作者:Hein Trip and Juke S. Lolkema日期:06/20/2013,浏览量:7748,Q&A: 0
Many bacteria are auxotrophic for at least a number of amino acids for which they lack the biosynthetic pathways. The organisms are still able to grow in media containing free amino acids as the sole source of amino acid when transport systems for the free amino acids are present in the cytoplasmic membrane. A range of transport systems for ...
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Macromolecular Biosynthesis Assay for Evaluation of Influence of an Antimicrobial on the Synthesis of Macromolecules 采用大分子生物合成法评估抗菌剂对大分子合成的影响 作者:Justyna Nowakowska, Nina Khanna and Regine Landmann日期:06/20/2013,浏览量:10179,Q&A: 0
One of the most compelling approaches in the discovery of novel antimicrobials is screening of natural sources. In our publication we report on the activity of a compound 8-hydroxyserrulat-14-en-19-oic acid (EN4), a diterpene isolated from the Australian plant Eremophila neglecta. We evaluate its applicability for treatment of ...
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EdU labeling of Trypanosome Cells and Their Kinetoplast DNA (kDNA) EdU标记锥体虫细胞及其动质体DNA(kDNA) 作者:Jianyang Wang日期:06/20/2013,浏览量:8859,Q&A: 0
Trypanosome mitochondrial genome, known as Kinetoplast DNA (kDNA), is a massive network of interlocked DNA rings. The studies of kDNA replication and architecture are of major significance since kDNA is a valid drug target. However, DNA in procyclic trypanosomes can not be labeled with tracer concentrations of 3[H]-thymidine, possibly ...
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IFN-α Inhibition Assay in vitro IFN-α 抑制的体外试验 作者:Kathrin Gibbert日期:06/20/2013,浏览量:8798,Q&A: 0
During viral infections Interferon-α (IFN-α) is expressed by infected host cells. IFN-α binds to its receptor (IFNAR1/2), which leads to the activation of downstream signaling via JAK-STAT. This signaling cascade results in the expression of several hundred different genes, so called interferon-stimulated gene, which lead to an antiviral state of ...
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Measurement of IFN-α Subtype Concentrations (Virus-free, Cell-based Bioassay) IFN-α亚型浓度的测定(无病毒,基于细胞的生物分析) 作者:Kathrin Gibbert日期:06/20/2013,浏览量:7585,Q&A: 0
The induction of type I IFN is the immediate host response against viral infections. Type I IFNs belong to a multigene family including up to 14 different IFN-α subtypes and one IFN-β. They are highly conserved and bind the same receptor (IFNAR1/2) with varying affinities, although they differ in their biological activities.
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分子生物学

Structural Based Strategy for Predicting Transcription Factor Binding Sites 采用结构型策略预测转录因子结合位点 作者:Beisi Xu, Yongmei Wang, Haojun Liang and Guohui Li日期:06/20/2013,浏览量:7804,Q&A: 0
Scanning through genomes for potential transcription factor binding sites (TFBSs) is becoming increasingly important in this post-genomic era. The position weight matrix (PWM) is the standard representation of TFBSs utilized when scanning through sequences for potential binding sites. Many transcription factor (TF) motifs are short and highly ...
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植物科学

Hairy Root Transformation in Lotus japonicus 百脉根(L. japonicus)的根毛转基因 作者:Satoru Okamoto, Emiko Yoro, Takuya Suzaki and Masayoshi Kawaguchi日期:06/20/2013,浏览量:17003,Q&A: 2
In L. japonicus, hairy root transformation is the very useful technique to generate transformed root systems in a short term. This protocol was previously described (Kumagai and Kouchi, 2003) with some modifications. After the infection of Agrobacterium rhizogenes, L. japonicus develops not only transformed but also ...
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Stable Transformation in Lotus japonicus 百脉根(L. japonicus)稳定转基因方法 作者:Takema Sasaki, Takuya Suzaki and Masayoshi Kawaguchi日期:06/20/2013,浏览量:13770,Q&A: 1
This is a protocol to produce stable transgenic plants in Lotus japonicus, which is established based on methods previously reported (Handberg and Stougaard, 1992; Stiller et al., 1997; Thkjaer et al., 1998) with some modifications. In this protocol, hygromycin is used to select transgenic plants.
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Growth Assay and Detection of TRP and Indole Derivatives in Piriformospora indica Culture Supernatant by LC-MS/MS TRP对印度梨形孢(P. indica)生长的影响与其代谢产物IAA及吲哚类衍生物的检测(LC-MS/MS) 作者:Magdalena Hilbert, Lars M. Voll, Jörg Hofmann and Alga Zuccaro日期:06/20/2013,浏览量:11254,Q&A: 0
The mutualistic root endophyte Piriformospora indica colonizes a wide range of plants and the colonization of root cells by this fungus is very often associated with beneficial effects to its host, such as growth promotion and increased biotic and abiotic stress tolerance. These traits may be based on general mechanisms and signaling ...
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Indole Derivative Feeding Test and Detection of TRP and Indole derivatives by Thin Layer Chromatography 吲哚衍生物的喂养试验及薄层色谱法检测TRP和吲哚衍生物 作者:Magdalena Hilbert, Lars M. Voll, Jörg Hofmann and Alga Zuccaro日期:06/20/2013,浏览量:9356,Q&A: 0
The mutualistic root endophyte Piriformospora indica colonizes a wide range of plants and the colonization of root cells by this fungus is very often associated with beneficial effects to its host, such as growth promotion and increased biotic and abiotic stress tolerance. These traits could be based on general mechanisms and signaling ...
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系统生物学

RNA-Seq Library Generation from Rare Human Cells Isolated by FACS 采用FACS法分离稀有人体细胞及其RNA测序文库的构建 作者:Sofia Gkountela and Amander T. Clark日期:06/20/2013,浏览量:11263,Q&A: 0
High throughput RNA Sequencing has revolutionized transcriptome analyses. However, most available protocols require micrograms of RNA rendering this technique not feasible for analyzing small numbers of cells, including precious rare cell types isolated from human tissues or organs. Here, we used an RNA Amplification System and describe a method ...
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