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细胞生物学

Harvest and Culture of Mouse Peritoneal Macrophages 小鼠腹水中巨噬细胞的采集和培养 作者:Mingfang Lu and Alan W. Varley日期:11/20/2013,浏览量:34230,Q&A: 1
Peritoneal macrophages are used as primary macrophages in lots of studies, mainly because they are easy to obtain. Injection of thioglycollate broth i.p. induces inflammatory responses and elicits large numbers of macrophages. This protocol can be used for harvesting resident or thioglycollate-elicited peritoneal cells. Peritoneal macrophages are ...
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免疫学

Gastric Aspiration Models 胃内容物的气管吸入模型 作者:Bruce A. Davidson and Ravi Alluri日期:11/20/2013,浏览量:17291,Q&A: 0
The procedures described below are for producing gastric aspiration pneumonitis in mice with alterations for rats and rabbits described parenthetically. We use 4 different injury vehicles delivered intratracheally to investigate the inflammatory responses to gastric aspiration:
1) Normal saline (NS) as the injury vehicle control ...
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Immunocytochemical Detection of Recombinant Biomphalysin on Schistosoma mansoni Sporocysts 免疫细胞化学法检测曼氏血吸虫孢囊中的重组毒素 作者:David Duval, Richard Galinier, Julien Portela, Guillaume Mitta and Benjamin Gourbal日期:11/20/2013,浏览量:7678,Q&A: 0
Schistosomiasis, or bilharzia, is a tropical disease caused by worms of the genus Schistosoma which infect about 200 million people. The life cycle of the parasite requires Biomphalaria, a specific genus of freshwater snails, as intermediate. Using an interactome approach employing B. glabrata plasma and S. mansoni ...
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Assessment of Human Dendritic Cell Antigen Uptake by Flow Cytometry 采用流式细胞术评估人树突细胞的抗原摄取 作者:Ana Luque, Sonia Cárdenas-Brito, Rut Olivar and Josep M. Aran日期:11/20/2013,浏览量:14855,Q&A: 0
Antigen uptake by dendritic cells is the first key step towards induction of antigen-specific T-cell responses. This flow cytometry-based protocol describes the analysis of dendritic cell uptake of soluble antigens through two different mechanisms: non-specific macropinocytosis (using Lucifer Yelloy CH), and receptor-mediated endocytosis (using DQ ...
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微生物学

Separation and Detection of Phosphorylated and Nonphosphorylated BvgA, a Bordetella pertussis Response Regulator, in vivo and in vitro 体内和体外分离和检测一种百日咳杆菌反应调节蛋白BvgA的磷酸化和非磷酸化形式 作者:Qing Chen, Alice Boulanger, Deborah M. Hinton and Scott Stibitz日期:11/20/2013,浏览量:9580,Q&A: 0
Protein phosphorylation plays a central role in signal transduction in bacteria. However, separation and detection of the phosphorylated protein from its nonphosphorylated form remain challenging. Here we describe a method to detect phosphorylation of the Bordetella pertussis response regulator BvgA, which is phosphorylated at an ...
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Transport Assays in Aspergillus nidulans 巢状曲霉转运蛋白动力学分析实验 作者:Emilia Krypotou and George Diallinas日期:11/20/2013,浏览量:7203,Q&A: 0
Transport assays allow the direct kinetic analysis of a specific transporter by measuring apparent Km and Vmax values, and permit the characterization of substrate specificity profiles through competition assays. In this protocol, we describe a rapid and easy method for performing uptake assays in the model ...
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Shigella IpaD and IpaB Surface Localizations 志贺氏杆菌IPaD和IPAB表面定位 作者:Lionel Schiavolin, Alaeddine Meghraoui and Abdelmounnaim Allaoui日期:11/20/2013,浏览量:9108,Q&A: 1
Shigella uses a type III secretion system to invade host cell and to cause disease. Secretion control and insertion of a translocation pore into cell membrane are critical steps for pathogenesis and are tightly linked to the formation of the needle tip complex formed by the IpaB and IpaD proteins (Veenendaal et al., 2007). ...
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Assay to Evaluate Vascular Permeability Induction in Mice 小鼠血管通透性诱导的分析评估 作者:Henry Puerta-Guardo, Arturo Raya-Sandino, Lorenza González-Mariscal, Victor H. Rosales, José Ayala-Dávila, Bibiana Chávez-Mungía, Daniel Martínez-Fong, Fernando Medina, Juan E. Ludert and Rosa María del Angel日期:11/20/2013,浏览量:20090,Q&A: 0
Dengue virus infection usually courses as a benign self-limited fever, called dengue fever. However, on occasions it can progress to a life-threatening complication known as severe dengue (SD). A hallmark of SD is a sharp increase in vascular permeability. Secondary infections are considered a risk factor to develop SD, presumably through a ...
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神经科学

Cell Cycle Analysis in the Vertebrate Brain Using Immunolabeled Fresh Cell Nuclei 在脊椎动物脑部采用荧光标记新鲜细胞核进行细胞周期分析 作者:Noelia Lopez-Sanchez and Jose M. Frade日期:11/20/2013,浏览量:14842,Q&A: 0
Flow cytometry, a standard technique used for quantitative analysis of isolated cells, is routinely employed by immunologists and oncologists to study DNA content, protein expression, and other functional parameters in blood and tumor cells. Unfortunately, the use of this technique by neurobiologists has been hampered by the complexity of the ...
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植物科学

Mapping and Analysis of Illumina Reads for Transcriptome of Medicago Truncatula During the Early Organogenesis of the Nodule 早期结瘤时期的苜蓿中的转录组分析(Illumina测序) 作者:Alexandre Boscari, Alberto Ferrarini, Jennifer del Giudice, Luca Venturini, Massimo Delledone and Alain Puppo日期:11/20/2013,浏览量:9372,Q&A: 0
Medicago truncatula serves as a model plant for legume genetics and genomics. We used RNA-Seq to characterize the transcriptome during the early organogenesis of the nodule and during its functioning. We generated approximately 135.5 million high-quality 36-bp reads, which were then aligned with the M. truncatula genome sequence ...
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Analysis of RNA-protein Interactions Using Electrophoretic Mobility Shift Assay (Gel Shift Assay) EMSA实验(凝胶迁移试验)分析RNA-蛋白质的相互作用 作者:Saiprasad Goud Palusa and Anireddy S. N. Reddy日期:11/20/2013,浏览量:26052,Q&A: 5
RNA binding proteins (RBPs) play a crucial role in regulating gene expression at the post-transcriptional level at multiple steps including pre-mRNA splicing, polyadenylation, mRNA stability, mRNA localization and translation. RBPs regulate these processes primarily by binding to specific sequence elements in nascent or mature transcripts. There ...
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Cotton Ovules Culture and Analysis 棉花胚珠的培养和分析 作者:Jiafu Tan, Fenglin Deng, Wenxin Tang, Jie Han, Guo Kai, Lili Tu and Xianlong Zhang日期:11/20/2013,浏览量:9062,Q&A: 0
The cotton ovules culture was innovated by Beasley and Ting (1973), and named after them. It is a convenient system to analyze the effect of chemical or environmental treatment on fiber development directly on ovules. This protocol was generated according to previous published papers and our practical experience.
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Shikimate Hydroxycinnamoyl Transferase (HCT) Activity Assays in Populus nigra 黑杨的莽草酸羟基肉桂转移酶(HCT)活性分析 作者:Igor Cesarino, Ruben Vanholme, Geert Goeminne, Bartel Vanholme and Wout Boerjan日期:11/20/2013,浏览量:12218,Q&A: 0
Lignin is a complex phenolic polymer deposited in secondarily-thickened plant cell walls. The polymer is mainly derived from the three primary monolignols: p-coumaryl, coniferyl and sinapyl alcohol which give rise to p-hydroxyphenyl, guaiacyl and syringyl units (H, G and S units, respectively) when coupled into the polymer. The building ...
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Bimolecular Fluorescence Complementation (BIFC) Protocol for Rice Protoplast Transformation 水稻原生质体中的(双分子荧光互补)BiFC实验 作者:Kun Wang, Ying Liu and Shaoqing Li日期:11/20/2013,浏览量:12539,Q&A: 0
After the plant cells are removed the cell walls by digestive enzyme, the plant protoplasts still have good cell activity. The protoplasts can be used to transiently express proteins of target genes in living plant cells through polyethylene glycol (PEG) mediated transformation. The purpose of this method is to employ the rice protoplasts and ...
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Binding Assay of Cytosolic Proteins to the Cytoskeleton 胞质蛋白与细胞骨架相互作用研究 作者:Stefano Del Duca and Giampiero Cai日期:11/20/2013,浏览量:10556,Q&A: 2
Many cellular proteins interact with the cytoskeleton (both actin filaments and microtubules), either dynamically or permanently. This interaction is required during different aspects of the cell life, for example during the process of cell division. In addition, many enzymes interact transiently with actin filaments and microtubules in order to ...
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