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癌症生物学

Invadopodia Detection and Gelatin Degradation Assay 侵足检测和明胶降解分析 作者:Begoña Díaz日期:12/20/2013,浏览量:19262,Q&A: 0
This protocol is designed to quantify invadopodia formation and activity. Invadopodia are protrusive structures elaborated by cancer cells that mediate cell attachment and remodeling of the extracellular matrix. These structures contribute to the ability of cancer cells to invade and metastasize. In this protocol, both the presence of invadopodia ...
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Measurement of Acetylcholine from Cell Lines 细胞系乙酰胆碱的测定 作者:Jamie K. Lau, Kathleen C. Brown and Piyali Dasgupta日期:12/20/2013,浏览量:8955,Q&A: 0
Cigarette smoking is the leading risk factor for the development of lung cancer. It is estimated that smoking is associated with 80-90% of lung cancer cases throughout the world (see References 1 and 2). The addictive component of cigarette smoke is nicotine. Our published data shows that nicotine promotes the production of acetylcholine (ACh) in ...
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细胞生物学

ImmunoFISH for Adherent Cultured Mammalian Cells 附着培养哺乳动物细胞的ImmunoFISH试验 作者:Francesca Rossiello, Marzia Fumagalli and Fabrizio d’Adda di Fagagna日期:12/20/2013,浏览量:9355,Q&A: 0
This protocol is optimized for immunoFISH staining of adherent cultured mammalian cells. It combines immunofluorescence for DNA damage response factors (e.g. 53BP1) and FISH against telomeric DNA.
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ImmunoFISH for Mice and Baboons Frozen Sections 小鼠和狒狒冰冻切片的ImmunoFISH试验 作者:Francesca Rossiello, Marzia Fumagalli and Fabrizio d’Adda di Fagagna日期:12/20/2013,浏览量:7439,Q&A: 0
This protocol is optimized for immunoFISH staining of OCT section of mouse tissues. It combines immunofluorescence for DNA damage response factors (e.g. 53BP1) (Le et al., 2010) and FISH against telomeric DNA.
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Autophagy Assays (LC3B immunofluorescence, LC3B western blot, acridine orange assay) 自体吞噬分析法(LC3B免疫荧光法、LC3B蛋白印迹法、吖啶橙分析法) 作者:Xin Zhang and Qingsong Liu日期:12/20/2013,浏览量:23369,Q&A: 1
Autophagy is a dynamic cellular event that is involved in the degradation of long lived proteins and organelles in cells. Biochemical methods such as western blot to measure autophagic proteins have been increasingly used in autophagy studies because it is convenient and objective. Among them, the total amount of Microtubule-associated protein ...
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免疫学

Influenza Virus-cell Fusion Inhibition Assay 流感病毒细胞融合抑制试验 作者:Mayo Yasugi and Kazuyoshi Ikuta日期:12/20/2013,浏览量:9120,Q&A: 0
During viral infection to host cells, several viruses undergo the process of endocytosis and pH-dependent fusion. By fusion of viral membrane with host cellular membrane, the viral core invades to host cytoplasm. A part of monoclonal antibodies against viral membrane protein have potential to inhibit the viral fusion step. Here we describe in ...
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Isolation of Neutralizing Antibody 中和抗体的分离 作者:Mayo Yasugi and Kazuyoshi Ikuta日期:12/20/2013,浏览量:7945,Q&A: 0
Use of monoclonal antibodies (MAbs) is an established laboratory strategy for characterization of specific pathogens and their antigenicity. Especially, Human MAbs (HuMAbs) with neutralizing activity against specific virus could have potential therapeutic application, and provide significant information on human epitopes that could be important ...
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Surface Plasmon Resonance Analysis of Antigen-Antibody Interaction 表面等离子共振分析抗原与抗体相互作用 作者:Mitsuhiro Nishimura and Kazuyoshi Ikuta日期:12/20/2013,浏览量:11237,Q&A: 0
Molecular interaction between monoclonal antibodies (MAbs) and their recognized antigen is a fundamental event leading to the neutralization activity. Estimation of their binding affinity gives beneficial information to characterize the MAbs and to develop more effective MAbs. Surface plasmon resonance (SPR) analysis is a powerful tool to analyze ...
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微生物学

EMS Mutagenesis of Clostridium difficile to Identify Strains with Germination-null Phenotypes EMS诱变鉴定艰难梭菌萌发无效显型菌株 作者:Michael B. Francis and Joseph A. Sorg日期:12/20/2013,浏览量:9571,Q&A: 0
Clostridium difficile is a Gram-positive, spore-forming, strict anaerobe and the leading cause of antibiotic-associated diarrhea (McFarland, 2008). Germination by C. difficile spores is the first step in pathogenesis. Thus, identifying mechanisms of C. difficile spore germination may lead to novel anti-germination ...
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Virulence Studies of Clostridium difficile 艰难梭菌的病毒性研究 作者:Michael B. Francis and Joseph A. Sorg日期:12/20/2013,浏览量:7184,Q&A: 0
Clostridium difficile (a Gram-positive, spore-forming, strict anaerobe) can colonize antibiotic-treated hosts (McFarland, 2008). Antibiotics alter the composition of the normal, benign microbial flora which leads to loss of colonization resistance (Wilson and Perini, 1988; Antonopoulos et al., 2009). C. difficile spores ...
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Colony Forming Assay for HCV-Replicon Cell Line 丙肝病毒复制子细胞系德尔集落形成分析法 作者:Chang Ho Lee and Seong-Wook Lee日期:12/20/2013,浏览量:8448,Q&A: 0
Hepatitis C virus (HCV) is the main causative agent of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. Since the HCV genome is present exclusively in RNA form during replication, a number of anti-HCV drugs show appearance of rapid drug-resistant viruses. Therefore, it is important to test generation of drug-escape mutant viruses ...
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神经科学

Ex utero Electroporation into Mouse Embryonic Neocortex 小鼠胚胎新皮质的宫外DNA电转移 作者:Branden R. Nelson日期:12/20/2013,浏览量:9997,Q&A: 0
This technique allows highly efficient and reproducible transfer of DNA/RNA into the embryonic neocortex of rodents across multiple ages. Ex utero electroporation compliments the more technically difficult in utero electroporation technique by maximizing the number of embryos for available for a given experiment, as well as ...
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植物科学

Observation of Chloroplast-actin Filaments in Leaves of Arabidopsis 拟南芥叶片中叶绿体-肌动蛋白微丝动态变化的观测 作者:Sam-Geun Kong and Masamitsu Wada 日期:12/20/2013,浏览量:8906,Q&A: 0
Chloroplast-actin (cp-actin) filaments play a pivotal role in chloroplast photorelocation movement. This protocol describes observation of cp-actin filaments in intact palisade cells of Arabidopsis leaves (Kong et al., 2013). The live cell imaging of cp-actin filaments is taken on moving chloroplasts, so that this protocol is ...
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Library Construction for Genome-wide Bisulfite Sequencing in Plants 拟南芥Bs-seq文库的构建 作者:David Moraga Amador, Chenggang Wang, Kevin H. Holland and Zhonglin Mou日期:12/20/2013,浏览量:12427,Q&A: 0
DNA methylation is the most studied epigenetic modification, which involves the addition of a methyl group to the carbon-5 position of cytosine residues in DNA. DNA methylation is important for the regulation of gene expression. Bisulfite sequencing is the gold standard technique for determining genome-wide DNA methylation profiles in eukaryotes. ...
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Membrane Preparation, Sucrose Density Gradients and Two-phase Separation Fractionation from Five-day-old Arabidopsis seedlings 利用蔗糖梯度密度离心的方法从拟南芥幼苗中分离膜蛋白 作者:Haibing Yang and Angus Murphy日期:12/20/2013,浏览量:21471,Q&A: 0
Membrane preparation has been widely used for characterization the membrane proteins. Membrane fractions can be separated by a combination of differential and density-gradient centrifugation techniques (Hodges et al., 1972; Leonard and Vanderwoude, 1976). Here we firstly describe a method to isolate total microsomal fractions including ...
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干细胞

Preparation of Adult Mouse Muscle Stem Cells 成年小鼠肌肉干细胞的制备 作者:Ju Li日期:12/20/2013,浏览量:9099,Q&A: 0
Muscle stem cells are adult stem cells responsible for muscle development, growth and regeneration. Current knowledge suggests those cells are heterogeneous population shared their position between the sarcolemma and basal lamina of muscle fibers. This protocol describes the technique to dissociate and collect the stem cells from skeletal muscle ...
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Intestinal Differentiation of Human ESCs 人胚胎干细胞在肠道中的分化 作者:Soichiro Ogaki and Shoen Kume日期:12/20/2013,浏览量:6673,Q&A: 0
ES cells (ESCs) are pluripotent and offer a good tool to study early embryonic development. Intestinal cells are derived from the definitive endoderm. In contrast to adult tissue stem cells, embryonic development and differentiation from ES cells have not been as well investigated in the intestine. There are four differentiated cell types of ...
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Intestinal Differentiation of Mouse ESCs 小鼠胚胎干细胞在肠道中的分化 作者:Soichiro Ogaki and Shoen Kume日期:12/20/2013,浏览量:6799,Q&A: 0
ES cells (ESCs) are pluripotent and offer a good tool to study early embryonic development. Intestinal cells are derived from the definitive endoderm. In contrast to adult tissue stem cells, embryonic development and differentiation from ES cells have not been as well investigated in the intestine. There are four differentiated cell types of ...
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