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癌症生物学

Detection of Tumor Cell Surface-reactive Antibodies 肿瘤细胞膜结合性抗体的检测 作者:Brian M. Andersen, Michelle R. Goulart, Michael R. Olin and G. Elizabeth Pluhar日期:05/05/2014,浏览量:7060,Q&A: 0
Vaccine-based immunotherapy is being used to treat dogs with primary brain tumors. The vaccines are composed of a lysate of autologous tumor cells, which stimulate an immune response producing tumor specific antibodies that are capable of inducing antibody-dependent cell-mediated cytotoxicity to allogeneic, as well as autologous, tumor cells. This ...
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Immunostaining Protocol: P-Smad2 (Xenograft and Mice) 免疫染色法:P-Smad2(磷酸化-Smad2,异种移植和小鼠) 作者:Alexandre Calon, Elisa Espinet, Sergio Palomo-Ponce, Daniele V. F. Tauriello, Mar Iglesias, María Virtudes Céspedes, Marta Sevillano, Cristina Nadal, Peter Jung, Xiang H.-F. Zhang, Daniel Byrom, Antoni Riera, David Rossell, Ramón Mangues, Joan Massague, Elena Sancho and Eduard Batlle日期:05/05/2014,浏览量:6860,Q&A: 0
Metastasis depends on a gene program expressed by the tumor microenvironment upon TGF-beta stimulation. CRC (Colorectal cancer) cell lines did not induce robust stromal TGF-beta responses when injected into nude mice as shown by lack of p-SMAD2 accumulation in tumor-associated stromal cells. To enforce high TGF-beta signaling in xenografts, we ...
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Immunostaining Protocol: P-Stat3 (Xenograft and Mice) 免疫染色法:P-Smat3(磷酸化-Smad3,异种移植和小鼠) 作者:Alexandre Calon, Elisa Espinet, Sergio Palomo-Ponce, Daniele V. F. Tauriello, Mar Iglesias, María Virtudes Céspedes, Marta Sevillano, Cristina Nadal, Peter Jung, Xiang H.-F. Zhang, Daniel Byrom, Antoni Riera, David Rossell, Ramón Mangues, Joan Massague, Elena Sancho and Eduard Batlle日期:05/05/2014,浏览量:9056,Q&A: 0
We sought to understand the mechanisms behind the potent effect of stromal TGF-beta program on the capacity of colorectal cancer (CRC) cells to initiate metastasis. We discovered that mice subcutaneous tumors and metastases generated in the context of a TGF-beta activated microenvironment displayed prominent accumulation of p-STAT3 in CRC cells ...
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Transwell Co-culture of Bone Marrow Macrophages with Tumor Cells 骨髓巨噬细胞和肿瘤细胞的跨室共培养 作者:Mackenzie K. Herroon and Izabela Podgorski日期:05/05/2014,浏览量:19314,Q&A: 0
Bone is a primary site of metastasis from prostate and breast cancers. Bone marrow macrophages (BMMs) are mediators of inflammatory processes and are thought to promote tumor growth in the skeletal sites. In order to elucidate how their interactions with tumor cells impact aggressiveness of metastatic tumors in bone in vitro methods are ...
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微生物学

Amino Acid Racemase Enzyme Assays 氨基酸消旋酶分析 作者:Atanas D. Radkov and Luke A. Moe日期:05/05/2014,浏览量:8047,Q&A: 0
Amino acid racemases are enzymes that invert the α-carbon stereochemistry of amino acids (AAs), interconverting amino acids between their L- and D-enantiomers in a reversible reaction. In bacteria, they are known to have catabolic physiological functions but are also involved in the synthesis of many D-AAs, including D-glutamate and D-alanine, ...
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Extraction and Quantification of Poly P, Poly P Analysis by Urea-PAGE 提取和量化多磷酸盐以及使用尿素聚丙烯酰胺凝胶(Urea-PAGE)对其进行分析 作者:Maria R. Gomez Garcia日期:05/05/2014,浏览量:8148,Q&A: 1
Inorganic polyphosphate (poly P) molecules, linear chains containing hundreds of orthophosphate (Pi) residues linked by high-energy phosphoanhydride bonds are abundant in every cell in nature. These molecules are widely distributed among bacteria, including key pathogens, and eukaryotes, poly P is present in organelles, including nuclei, ...
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Minimal Bactericidal Concentration for Biofilms (MBC-B) 体外微量滴定测定法测定生物膜的最小杀菌浓度(MBC-B) 作者:Nicole Billings and Katharina Ribbeck日期:05/05/2014,浏览量:11150,Q&A: 1
A biofilm is a multicellular consortium of surface associated microbes surrounded by a hydrated, extracellular polymer matrix. The biofilm matrix plays a critical role in preventing desiccation, acquiring nutrients, and provides community protection from environmental assaults. Importantly, biofilms are significantly more resistant to ...
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Microfluidic-based Time-kill Kinetic Assay 基于微流体的时间与杀菌关系的动力学分析 作者:Nicole Billings, Roberto Rusconi, Roman Stocker and Katharina Ribbeck日期:05/05/2014,浏览量:10795,Q&A: 0
In many environments, bacteria favor a sessile, surface-attached community lifestyle. These communities, termed biofilms, are ubiquitous among many species of bacteria. In some cases, biofilms form under flow conditions. Flow chambers, and in particular microfluidic channels, can be used to observe biofilm development and physiological effects ...
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Enterovirus 71 Virus Propagation and Purification 肠道病毒71的增殖和纯化 作者:Kristin L. Shingler, Lindsey J. Organtini and Susan Hafenstein日期:05/05/2014,浏览量:12202,Q&A: 0
Since its discovery in 1969, enterovirus 71 (EV71) has emerged as a serious worldwide health threat. This member of the picornavirus family causes hand, foot, and mouth disease, and also has the capacity to invade the central nervous system to cause severe disease and death. This is the propagation and purification procedure to produce infectious ...
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ELISA for Alpha-hemolysin (Hla) in Methicilin-resistant Staphylococcus aureus (MRSA) 酶联免疫吸附试验分析耐甲氧西林葡萄球菌属(MRSA)中的α溶血素(Hla) 作者:Varandt Y. Khodaverdian and Menachem Shoham日期:05/05/2014,浏览量:8784,Q&A: 0
Anti-virulence agents against MRSA inhibit the production of disease-causing virulence factors, such as alpha-hemolysin, but are neither bacteriostatic nor bactericidal. Here we discuss a rapid method to screen for MRSA anti-virulence agents by measuring alpha-hemolysin production through ELISA. This protocol can be used with other alpha-hemolysin ...
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In vitro Analysis for Macrophage Binding and Pro-inflammatory Responses to Candida albicans 巨噬细胞对白色念珠菌的结合和促炎反应的体外分析方法 作者:Mohlopheni J. Marakalala日期:05/05/2014,浏览量:7419,Q&A: 0
Macrophage recognition of Candida albicans (C. albicans) is facilitated by pattern recognition receptors that interact with the fungal pathogen associated molecular patterns (PAMPs). Dectin-1 is the major macrophage receptor that is known to recognize fungal Beta-glucans leading to induction of various immune responses. This ...
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植物科学

Extraction and Measurement the Activities of Cytosolic Phosphoenolpyruvate Carboxykinase (PEPCK) and Plastidic NADP-dependent Malic Enzyme (ME) on Tomato (Solanum lycopersicum) 番茄胞质中的磷酸烯醇丙酮酸羧激酶(PEPCK)和质体中的NADP依赖性苹果酸酶(ME)的提取和活性测定 作者:Sonia Osorio, José G. Vallarino, Marek Szecowka, Shai Ufaz, Vered Tzin, Ruthie Angelovici, Gad Galili and Alisdair R. Fernie日期:05/05/2014,浏览量:9200,Q&A: 0
A recent study demonstrated that cytosolic phosphoenolpyruvate carboxykinase (PEPCK) and NADP-malic enzyme (NADP-ME) have an important role in malate metabolism during fruit ripening (Osorio et al., 2013). PEPCK catalyze the ATP-dependent decarboxylation of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) and NADP-ME, the reversible ...
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