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癌症生物学

Lysosomal Stability Assay 溶酶体稳定性分析 作者:Nikolaj H. T. Petersen, Thomas Kirkegaard and Marja Jäättelä日期:06/20/2014,浏览量:15086,Q&A: 0
This assay makes use of the dye Acridine Orange (AO) to determine the stability of lysosomes in living cells upon exposure to a confocal microscope laser.

AO is a lipophilic amine that readily diffuses into cells (Figure 1). Inside the cell it enters the acidic lysosomal compartment where it is protonated and sequestered, shifting its ...
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细胞生物学

EML Erythroid and Neutrophil Differentiation Protocols 骨髓淋巴样红细胞(EML)中的红血球和噬中性细胞分化实验流程 作者:Cristina Pina, Cristina Fugazza and Tariq Enver日期:06/20/2014,浏览量:10210,Q&A: 0
Erythroid-Myeloid-Lymphoid cells (EML) are a multipotent haematopoietic cell line of mouse bone marrow origin capable of long-term maintenance in vitro in the presence of SCF (stem cell factor) (Tsai et al., 1994). The self-renewal capacity of the EML cell line is conferred by the presence of a dominant-negative retinoic acid ...
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免疫学

Bone Marrow Derived Eosinophil Cultures 骨髓源嗜酸性细胞培养 作者:Thomas X. Lu and Marc E. Rothenberg日期:06/20/2014,浏览量:11784,Q&A: 1
Eosinophils are multifunctional effector cells implicated in the pathogenesis of a variety of diseases including asthma, eosinophil gastrointestinal disorders and helminth infection. Mouse bone marrow derived progenitor cells can be differentiated into eosinophils following IL-5 exposure. These bone marrow derived eosinophils are fully ...
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微生物学

A SYBR Green-based Real Time RT-PCR Assay for Detection of the Emerging H7N9 Virus 基于SYBR Green荧光染料的实时逆转录聚合酶链式反应(RT-PCR)试验检测新生 H7N9 病毒 作者:Zheng Zhu and Lunbiao Cui日期:06/20/2014,浏览量:8185,Q&A: 0
Most recently a novel avian-origin influenza A (H7N9) virus emerged in China and has been associated with lots of human infection and fatal cases. Molecular diagnostic methods are thus urgently needed in public health laboratories. We developed a SYBR green-based one-step real time reverse transcription-PCR (RT-PCR) to detect the novel H7N9 virus.
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Determination of Oxygen Respiration Rates in Wetted Developmentally Arrested Spores of Streptomyces Species 链霉菌属物种潮湿抑制发育孢子的氧气呼吸率测定 作者:Marco Fischer, Dörte Falke and R. Gary Sawers日期:06/20/2014,浏览量:7467,Q&A: 0
Streptomyces species produce spores, which, while not as robust as endospores of Bacillus or Clostridium species, are capable of surviving for months or even years (Hopwood, 2006). During this time these spores remain viable, surviving by slowly degrading internal stores of carbon compounds, such as the carbohydrate ...
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Rapid Nitrate Reduction Assay with Intact Microbial Cells or Spores 应用完整微生物细胞或孢子进行硝酸快速还原分析 作者:Marco Fischer, Dörte Falke and R. Gary Sawers日期:06/20/2014,浏览量:7412,Q&A: 0
Many microorganisms have the capacity to use nitrate as a respiratory electron acceptor. Reduction of nitrate is catalyzed by a multi-subunit nitrate reductase that is often located associated with the cytoplasmic membrane and has its active site oriented toward the cytoplasm. This means that nitrate must be transported into the cell and often ...
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Analysis of Mycobacterial Protein Secretion 分枝杆菌蛋白质分泌物的分析 作者:Alka Mehra and Jennifer A. Philips日期:06/20/2014,浏览量:11545,Q&A: 0
Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis. Analysis of proteins secreted by Mtb has been of interest to the field of tuberculosis research since certain secreted proteins interact with the host to promote virulence, while others may be important antigens or serve as biomarkers of infection. Here, we describe ...
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植物科学

Protoplast Preparation and Determination of Cell Death 原生质体制备和细胞死亡的鉴定 作者:Antoine Danon日期:06/20/2014,浏览量:15878,Q&A: 3
The protoplasts assay constitutes a powerful tool that allows an easy uptake of active agents and a precise quantification of cell death induction in different populations. Our study showed that the basal level of cell death in our controls is low and stable throughout the length of our experiments (Danon et al., 2005; Pineau et al. ...
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MTV1 Pull-down Assay in Arabidopsis 拟南芥MTV1 的pull-down实验 作者:Michael Sauer日期:06/20/2014,浏览量:10306,Q&A: 0
This protocol is an example of how to analyse suspected interactions between proteins using a pull-down assay (Sauer et al., 2013). A bait protein of interest (in this case, MTV1 of Arabidopsis thaliana) is fused to a GST tag and expressed in bacteria. The protein is isolated and allowed to bind to a matrix of ...
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Deflagellation and Regeneration in Chlamydomonas 衣藻的鞭毛脱落和再生 作者:William Dentler日期:06/20/2014,浏览量:13085,Q&A: 0
Eukaryotic cilia/flagella are one of the only cellular structures that can be removed without injuring cells, can be highly purified for biochemical analysis, and, in many cells, can be completely reassembled within 90 minutes. Following amputation, the expression of many flagellar genes is up-regulated, and many are packaged and associated with ...
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Ciliary and Flagellar Membrane Vesicle (Ectosome) Purification 纤毛和鞭毛囊泡(外皮层)的纯化 作者:William Dentler日期:06/20/2014,浏览量:8717,Q&A: 0
Eukaryotic cilia/flagella are ideal organelles for the analysis of membrane trafficking, membrane assembly, and the functions of a variety of signal transduction molecules. Cilia are peninsular organelles and the membrane lipids, membrane proteins, and microtubular-associated components are selectively transported into cilia through the region ...
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In organello Protein Synthesis 细胞器的蛋白质合成 作者:Malgorzata Kwasniak-Owczarek and Hanna Janska日期:06/20/2014,浏览量:11389,Q&A: 1
In organello protein synthesis method allows the analysis of mitochondrial translation products. The principle of this method relies on incubation of isolated intact mitochondria with radiolabeled amino acids such as 35S methionine. After protein synthesis, the radiolabeled translation products are subsequently separated by SDS ...
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Determination of Luciferase Activity in Arabidopsis seedling 拟南芥幼苗中荧光素酶活性的测定 作者:Mohan TC, Gabriel Castrillo and Antonio Leyva日期:06/20/2014,浏览量:10873,Q&A: 0
There are different direct and indirect methods available to study gene expression in plant systems. In this protocol we describe a modified expression assay using transgenic plants expressing the luciferase gene under the control of phosphate transporter PHT1;1 promoter. This assay was originally optimized for analyzing the repression of PHT1;1 ...
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系统生物学

ChIP-Seq in Candida albicans 白色念珠菌的染色质免疫共沉淀测序(ChIP-Seq) 作者:Sadri Znaidi, Caroline Proux, Sandra Weber, Simon Drouin, François Robert, Martine Raymond, Jean-Yves Coppée and Christophe d’Enfert日期:06/20/2014,浏览量:15225,Q&A: 0
Systems biology approaches can be used to study the regulatory interactions occurring between many components of the biological system at the whole-genome level and decipher the circuitries implicated in the regulation of cellular processes, including those imparting virulence to opportunistic fungi. Candida albicans (C. albicans ...
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