植物

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    现刊
    A β-glucuronidase (GUS) Based Bacterial Competition Assay to Assess Fine Differences in Fitness during Plant Infection
    基于β-葡萄糖醛酸酶 (GUS) 的细菌竞争测定以评估植物感染期间适应性的细微差异
    作者:Julien S. Luneau, Laurent D. Noël, Emmanuelle Lauber and Alice Boulanger日期:07/05/2022,浏览量:884,Q&A: 0
    [Abstract]

    Competition assays are a simple phenotyping strategy that confront two bacterial strains to evaluate their relative fitness. Because they are more accurate than single-strain growth assays, competition assays can be used to highlight slight differences that would not otherwise be detectable. In the frame of host-pathogens interactions, they can be

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    Quantitative Live Confocal Imaging in Aquilegia Floral Meristems
    白葵花分生组织的定量实时共聚焦成像
    作者:Ya Min, Stephanie J. Conway and Elena M. Kramer日期:06/20/2022,浏览量:1242,Q&A: 0
    [Abstract]

    In this study, we present a detailed protocol for live imaging and quantitative analysis of floral meristem development in Aquilegia coerulea, a member of the buttercup family (Ranunculaceae). Using confocal microscopy and the image analysis software MorphoGraphX, we were able to examine the cellular growth dynamics during floral organ primordia

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    Quantitative Analysis of Redox Pool (NAD+, NADH Content) in Plant Samples Under Aluminum Stress
    铝胁迫下植物样品氧化还原池(NAD+, NADH含量)的定量分析
    作者:Jay Prakash Awasthi, Bedabrata Saha, Hiroyuki Koyama and Sanjib Kumar Panda日期:06/20/2022,浏览量:1238,Q&A: 0
    [Abstract]

    Nicotinamide adenine dinucleotide (NAD) is an essential cofactor of numerous enzymatic reactions found in all living cells. Pyridine nucleotides (NAD+ and NADH) are also key players in signaling through reactive oxygen species (ROS), being crucial in the regulation of both ROS-producing and ROS-consuming systems in plants. NAD content is a

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    Investigation of Transposon DNA Methylation and Copy Number Variation in Plants Using Southern Hybridisation
    利用Southern杂交技术研究植物中转座子DNA甲基化和拷贝数变异的情况
    作者:Vivek Hari Sundar G. and P. V. Shivaprasad日期:06/05/2022,浏览量:731,Q&A: 0
    [Abstract]

    Plant genomes are pronouncedly enriched in repeat elements such as transposons. These repeats are epigenetically regulated by DNA methylation. Whole genome high-depth sequencing after bisulfite treatment remains an expensive and laborious method to reliably profile the DNA methylome, especially when considering large genomes such as in crops.

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    Quantification of Soil-surface Roots in Seedlings and Mature Rice Plants
    幼苗和成熟水稻植物土壤表面根的量化
    作者:Eiko Hanzawa, Yuka Kitomi, Yusaku Uga and Tadashi Sato日期:05/05/2022,浏览量:1255,Q&A: 0
    [Abstract]

    Soil-surface roots (SORs) in rice are primary roots that elongate over or near the soil surface. SORs help avoid excessive reduction of stress that occurs in paddy, such as in saline conditions. SORs may also be beneficial for rice growth in phosphorus-deficient paddy fields. Thus, SOR is a useful trait for crop adaptation to certain environmental

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    A Molecular Cloning and Sanger Sequencing-based Protocol for Detecting Site-specific DNA Methylation
    一种基于分子克隆和Sanger测序的位点特异性DNA甲基化检测方案
    作者:Wei Guo, Anthony Cannon and Damon Lisch日期:05/05/2022,浏览量:905,Q&A: 1
    [Abstract]

    DNA methylation is a conserved chemical modification, by which methyl groups are added to the cytosine of DNA molecules. Methylation can influence gene expression without changing the sequence of a particular gene. This epigenetic effect is an intriguing phenomenon that has puzzled biologists for years. By probing the temporal and spatial patterns

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    Apoplastic Expression of CARD1-ecto Domain in Nicotiana benthamiana and Purification from the Apoplastic Fluids
    本氏烟草中 CARD1-ecto 结构域的质外体表达及质外体液的纯化
    作者:Nobuaki Ishihama, Anuphon Laohavisit, Kaori Takizawa and Ken Shirasu日期:04/20/2022,浏览量:1163,Q&A: 0
    [Abstract]

    The protein expression and purification process is an essential initial step for biochemical analysis of a protein of interest. Traditionally, heterologous protein expression systems (such as E. coli, yeast, insect cells, and cell-free) are employed for plant protein expression, although a plant expression system is often desirable for plant

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    Application of Cadaverine to Inhibit Biotin Biosynthesis in Plants
    尸胺在抑制植物生物素合成中的应用
    作者:Nicole M. Gibbs, Shih-Heng Su and Patrick H. Masson日期:04/20/2022,浏览量:678,Q&A: 0
    [Abstract]

    Biotin is an essential vitamin in plants. However, characterization of biotin deficiency has been limited by embryo lethality in mutants, which can only be rescued by supplementation of biotin. Here, we describe a protocol to characterize biotin deficiency in Arabidopsis thaliana through application of the polyamine cadaverine. Cadaverine

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    In vitro Auto- and Substrate-Ubiquitination Assays
    体外自体和底物泛素化测定
    作者:Hye Lin Park, Han Yong Lee and Gyeong Mee Yoon日期:04/05/2022,浏览量:810,Q&A: 1
    [Abstract]

    The precise regulation of the homeostasis of the cellular proteome is critical for the appropriate growth and development of plants. It also allows the plants to respond to various environmental stresses, by modulating their biochemical and physiological aspects in a timely manner. Ubiquitination of cellular proteins is one of the major

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    Activity-based Protein Profiling of Serine Hydrolase Superfamily Enzymes
    丝氨酸水解酶超家族酶的基于活性的蛋白质分析
    作者:Achintya Kumar Dolui, Mahadev Latha and Panneerselvam Vijayaraj日期:03/20/2022,浏览量:750,Q&A: 0
    [Abstract]

    Activity-based protein profiling (ABPP) is a chemoproteomics platform to assess the functional state of enzymes in complex biological systems. Over the two decades, ABPP has emerged from a gel-based to gel-free platform, for in-depth proteome analysis with enhanced resolution, sensitivity for target detection, and discovery of small molecule

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