原核生物

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    现刊
    Combining Gel Retardation and Footprinting to Determine Protein-DNA Interactions of Specific and/or Less Stable Complexes
    结合凝胶阻滞和足印法测定特异性和/或不稳定复合物的蛋白质-DNA相互作用
    作者:Meng-Lun Hsieh, Alice Boulanger, Leslie G. Knipling and Deborah M. Hinton日期:12/05/2020,浏览量:1661,Q&A: 0
    [Abstract]

    DNA footprinting is a classic technique to investigate protein-DNA interactions. However, traditional footprinting protocols can be unsuccessful or difficult to interpret if the binding of the protein to the DNA is weak, the protein has a fast off-rate, or if several different protein-DNA complexes are formed. Our protocol differs from traditional

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    Structural Analysis of Bordetella pertussis Biofilms by Confocal Laser Scanning Microscopy
    利用激光扫描共聚焦显微镜研究百日咳杆菌生物膜的结构
    作者:Natalia Cattelan, Osvaldo Miguel Yantorno and Rajendar Deora日期:08/05/2018,浏览量:5123,Q&A: 0
    [Abstract] Biofilms are sessile communities of microbial cells embedded in a self-produced or host-derived exopolymeric matrix. Biofilms can both be beneficial or detrimental depending on the surface. Compared to their planktonic counterparts, biofilm cells display enhanced resistance to killing by environmental threats, chemicals, antimicrobials and host ...
    Separation and Detection of Phosphorylated and Nonphosphorylated BvgA, a Bordetella pertussis Response Regulator, in vivo and in vitro
    体内和体外分离和检测一种百日咳杆菌反应调节蛋白BvgA的磷酸化和非磷酸化形式
    作者:Qing Chen, Alice Boulanger, Deborah M. Hinton and Scott Stibitz日期:11/20/2013,浏览量:10298,Q&A: 0
    [Abstract] Protein phosphorylation plays a central role in signal transduction in bacteria. However, separation and detection of the phosphorylated protein from its nonphosphorylated form remain challenging. Here we describe a method to detect phosphorylation of the Bordetella pertussis response regulator BvgA, which is phosphorylated at an ...