病毒

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    现刊
    Metal-tagging Transmission Electron Microscopy for Localisation of Tombusvirus Replication Compartments in Yeast
    使用金属标签透射电子显微技术定位酵母菌中番茄丛矮病毒复制区
    作者:Isabel Fernández de Castro and Cristina Risco日期:04/20/2018,浏览量:5470,Q&A: 0
    [Abstract] Positive-stranded (+) RNA viruses are intracellular pathogens in humans, animals and plants. To build viral replicase complexes (VRCs) viruses manipulate lipid flows and reorganize subcellular membranes. Redesigned membranes concentrate viral and host factors and create an environment that facilitates the formation of VRCs within replication ...
    Determination of the Secondary Structure of an RNA fragment in Solution: Selective 2`-Hydroxyl Acylation Analyzed by Primer Extension Assay (SHAPE)
    溶液中RNA片段二级机构的测定:引物延伸法(SHAPE)分析选择性2-羟基酰化
    作者:Manuel Miras, Raquel N. Sempere, Jelena J. Kraft, W. Allen Miller, Miguel A. Aranda and Veronica Truniger日期:01/20/2015,浏览量:12281,Q&A: 0
    [Abstract] This protocol describes the methodology for the determination of the secondary structure of an RNA fragment in solution using Selective 2´-Hydroxyl Acylation analyzed by Primer Extension, abbreviation SHAPE. It consists in the very fast chemical modification of flexible and therefore possibly single-stranded nucleotides in a sequence-independent ...