鱼类

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    现刊
    Measurement of DNA Damage Using the Neutral Comet Assay in Cultured Cells
    在培养细胞中使用中性彗星试验测量 DNA 损伤
    作者:Elena Clementi, Zuzana Garajova and Enni Markkanen日期:11/20/2021,浏览量:1871,Q&A: 0
    [Abstract]

    Maintenance of DNA integrity is of pivotal importance for cells to circumvent detrimental processes that can ultimately lead to the development of various diseases. In the face of a plethora of endogenous and exogenous DNA damaging agents, cells have evolved a variety of DNA repair mechanisms that are responsible for safeguarding genetic

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    Paper Lateral Flow Biosensor for Nodavirus Reverse Transcribed RNA Detection
    用于诺达病毒逆转录RNA检测的纸侧向流生物传感器
    作者:Dimitra K. Toubanaki and Evdokia Karagouni日期:08/05/2020,浏览量:2249,Q&A: 0
    [Abstract] Paper nanobiosensors have been established as an excellent platform for analysis of veterinary and human pathogens causing various diseases. Especially, lateral flow assays or biosensors ideal for sensitive, rapid, robust and accurate analysis in laboratory setups and on-site analysis. Viral RNA detection is of great importance for public health ...
    mRNA Extraction from Gill Tissue for RNA-sequencing
    鳃组织mRNA 提取用于RNA测序
    作者:Jukka-Pekka Verta and Felicity Jones日期:03/05/2020,浏览量:3612,Q&A: 0
    [Abstract] Adaptation is thought to proceed in part through spatial and temporal changes in gene expression. Fish species such as the threespine stickleback are powerful vertebrate models to study the genetic architecture of adaptive changes in gene expression since divergent adaptation to different environments is common, they are abundant and easy to study ...
    Quantification of Cutaneous Ionocytes in Small Aquatic Organisms
    小型水生生物皮肤离子细胞的定量分析
    作者:Garfield T. Kwan, Shane H. Finnerty, Nicholas C. Wegner and Martin Tresguerres日期:05/05/2019,浏览量:4330,Q&A: 0
    [Abstract] Aquatic organisms have specialized cells called ionocytes that regulate the ionic composition, osmolarity, and acid/base status of internal fluids. In small aquatic organisms such as fishes in their early life stages, ionocytes are typically found on the cutaneous surface and their abundance can change to help cope with various metabolic and ...
    Microplastic Extraction from Marine Vertebrate Digestive Tracts, Regurgitates and Scats: A Protocol for Researchers from All Experience Levels
    海洋脊椎动物消化道,反流物和粪便中塑料微粒的提取:一个适用于所有经验水平研究者的实验方法
    作者:Amy L. Lusher and Gema Hernandez-Milian日期:11/20/2018,浏览量:8323,Q&A: 0
    [Abstract] It is essential to provide a protocol for the separation and identification of microplastics in marine vertebrates (mammals, birds, turtles and fish) that is easy to follow and adaptable depending on research infrastructure. Digesting organic material is an effective way to analyze samples for microplastics. Presented here is an optimized protocol ...
    Fish Bile Clean-up for Subsequent Zymography and Mass Spectrometry Proteomic Analyses
    鱼胆汁清除后进行酶谱和质谱蛋白质组学分析
    作者:Rachel Ann Hauser-Davis日期:01/20/2018,浏览量:6454,Q&A: 0
    [Abstract] Biliary excretion offers a way to analyze various contaminants in aquatic organisms, and fish bile has been used as a biomarker for environmental contamination. The use of the fish bile proteome as a tool for monitoring the impact of environmental contaminants has been recently validated. However, scarce studies in this context are available, and ...